The present disclosure provides a method for cryopreserving corneal endothelial cells and/or corneal endothelial-like cells, and a freezing preparation for corneal endothelial cells and/or corneal endothelial-like cells. The present disclosure provides a method for preserving corneal endothelial cells and/or corneal endothelial-like cells, wherein the method includes: a freezing step for freezing unfrozen corneal endothelial cells and/or corneal endothelial-like cells, the freezing step including at least one stage in which the temperature is reduced at a rate of less than 1 C./min when the temperature is changed from the unfrozen temperature to the freezing target temperature; and, if necessary, a step for maintaining the corneal endothelial cells and/or the corneal endothelial-like cells in a frozen state.

Provided herein are enzymatic compositions for carbohydrate antigen cleavage, methods, uses, apparatuses and sys-O tems associated therewith. In particular, the composition comprises two enzymes, GalNAcDeacetylase and Galactosaminidase and the el composition may further comprise a crowding agent. Furthermore, the compositions described herein were found to have activity a C) temperatures and pH levels suitable for cell viability.

The present invention provides compositions and formulations comprising glutathione with or without thiocyanate and methods of use thereof to treat diseases and disorders in mucosal/epithelial tissue.

A sperm extender medium for prolonging the fertility of bovine spermatozoa during liquid storage of the spermatozoa. The medium includes a monosaccharide energy source for the spermatozoa. The one or more or amino acid osmolytes are selected from mono-amino carboxylic and mono-amino sulfonic acids, each independently having an R side group selected from the group consisting of H and non-polar, neutral open aliphatic chains excluding branched C3 and longer aliphatic chains. The medium also includes one or more antioxidants and physiologically acceptable inorganic salts for maintaining sperm viability, and is buffered for maintaining pH in a range of from about 7.0 to about 8.5. Further, the medium may have a concentration of sodium ions of less than about 95 mM, and an osmolarity in a range of from about 290 mOsm/L to about 400 mOsm/L. Preparations including the spermatozoa and methods for preparing the medium and methods for fertilisation of an ovum.

The disclosure provides, in various embodiments, systems, devices and methods relating to ex-vivo organ care. In certain embodiments, the disclosure relates to maintaining an organ ex-vivo at near physiologic conditions. In certain embodiments, the disclosure relates to placing an ex-vivo heart in a chamber of a portable organ care system; providing, via a pump, a pulsatile flow of a perfusion fluid to the ex-vivo heart; measuring, with a sensor, one or more physiologic characteristics of the ex-vivo heart while the ex-vivo heart is beating; and operating the pump in response to the one or more physiologic characteristics.

The present invention generally relates to compositions and methods for the handling of processed sperm populations including samples that are freshly collected, those transported as fresh samples, as well as samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the sperm cell. Such trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the sperm's ability to fertilize an egg, grow into a health embryo and produce a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals

The present disclosure provides oxygen-carrying nanoparticles, methods of making the nanoparticles, and methods of using the nanoparticles to carry oxygen in blood.

The present disclosure provides compositions, kits, and methods to protect organs by inducing acquired cytoresistance without causing injury to the organ. The compositions, kits, and methods utilize heme proteins, iron and/or vitamin B12 and, optionally, agents that impact heme protein metabolism.

The present disclosure provides compositions and methods for treating or preventing macular degeneration in a subject. The disclosure also provides compositions and methods for preserving organs and tissues for transplantation, and for preventing cellular injury in organs or in subjects.

Solutions and suspensions comprising polymerized hemoglobin derived from human blood are disclosed. The solutions and suspensions may comprise cell culture medium, an enzyme (such as a protease), and/or a buffer. Processes of preparing the solutions and suspensions are also disclosed. The solutions and suspensions may be employed in methods of isolating mammalian cells, such as pancreatic islets, methods of preserving mammalian tissue and organs, methods of aiding the recovery of mammalian cells following their isolation, methods of maintaining mammalian cells, methods of propagating mammalian cells, and methods of treating a mammal with diabetes.