The present invention relates to a method of co-culturing Inonotus obliquus, Ganoderma lucidum, and Phellinus linteus. The co-cultured mycelia prepared through the method of the present invention have high beta-glucan content and thus can exhibit superior health functionality, and can be used as an additive or a cooking seasoning in various foods. In addition, the use of the co-cultured mycelia in curing raw meat enables easy preparation of a meat-based food product that has a good taste and flavor.

Provided herein are shelf-stable protein food ingredients, food products comprising the shelf-stable protein food ingredients, methods of their production, and methods of their use. The shelf-stable protein food ingredients comprise cultured fungal biomass and a limited amount of water. Advantageously, the shelf-stable protein food ingredients can be stored, transported, and delivered within the food supply.

A mussel soybean paste and a preparation method thereof are provided, the preparation method includes: sterilizing soaked soybeans, adding mussel cooking liquor hydrolysate, and then inoculating Aspergillus oryzae for making koji to obtain soybean koji; sterilizing soaked rice, and inoculating Aspergillus oryzae for making koji to obtain rice koji; mixing the rice koji with the soybean koji to obtain a koji material; adding the koji material and salt into mussel meat for anaerobic fermentation, grinding an obtained mussel soybean paste semi-finished product by using a colloid mill, and conducting sterilizing to obtain the mussel soybean paste. The mussel soybean paste which is natural, nutritional and good in flavor can be prepared, and the added value of mussels is increased.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

According to exemplary embodiments, provided herein are methods for nutrient fortification, including determining a nutrient profile for a first food product, modeling a micronutrient profile based on the nutrient profile, culturing yeast in the micronutrient profile and applying the cultured yeast to a second food product.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

The present invention relates to compositions comprising biomass of a thermophilic fungus for use as an ingredient in food products, beverages, pet foods or animal feed. Due to its nutritional composition the biomass of the thermophilic fungus can be used as a source of protein and/or amino acids, including essential amino acids; lipids, including oleic and linoleic acids; dietary fiber; choline and vitamins such as vitamin E; and minerals such as iron. The biomass of the thermophilic fungus is available as a compressed biomass cake or as a dried powder. As such the biomass of the thermophilic fungus is suitable as ingredient in meat replacers, and in hybrid meat products. In the form of dried powder the biomass of the thermophilic fungus can be easily mixed with other ingredients to produce bakery products such as bread or soups.

The present invention relates to a composition for control of microbial development of an edible substance the composition comprises a supernatant comprising a bacteriocin produced from fermentation of a bacteriocin producing bacteria and an organic acid produced from fermentation of a bacteriocin producing bacteria, wherein organoleptic characteristics of the edible substance are not significantly altered.

Provided herein are shelf-stable protein food ingredients, food products comprising the shelf-stable protein food ingredients, methods of their production, and methods of their use. The shelf-stable protein food ingredients comprise cultured fungal biomass and a limited amount of water. Advantageously, the shelf-stable protein food ingredients can be stored, transported, and delivered within the food supply.