A method to visualize, display, analyze and quantify angiography, perfusion, and the change in angiography and perfusion in real time, is provided. This method captures image data sequences from indocyanine green near infra-red fluorescence imaging used in a variety of surgical procedure applications, where angiography and perfusion are critical for intraoperative decisions.

A system and method for depth-resolved imaging of fluorophore concentrations in tissue uses a pulsed light source stimulus wavelength to illuminate the tissue; and a time-gated electronic camera such as a single-photon avalanche detector camera to observe the tissue in multiple time windows after start of each light pulse. A filter-changer or tunable filter is between the tissue and the electronic camera with fluorescent imaging settings and a stimulus wavelength setting, and an image processor receives reflectance images and fluorescent emissions images from the time-gated camera and processes these images into depth and quantity resolved images of fluorophore concentrations in the tissue.

Embodiments may provide self-guided, self-directed diagnostics and treatment of neural conditions. For example, a system may comprise a processor, memory accessible by the processor, and program instructions and data stored in the memory, a plurality of stimulation devices connected to signal output circuitry interfacing the processor with the stimulation devices, program instructions and data to control the stimulation devices to generate and transmit stimulation signals, a plurality of sensing devices connected to signal input circuitry interfacing the processor with the sensing devices, program instructions and data to receive sensed signals from the sensing devices, a communication device adapted to wirelessly communicate with a server computer system, and program instructions and data to perform dynamic closed loop feedback of the stimulation signals based on the received sensed signals to provide self-guided, self-directed diagnostics and treatment of neural conditions using at least one recipe for a treatment strategy guided by artificial intelligence.

Provided is a method to detect dental composite materials by using a dental tool that provides a source of ultraviolet light. The method includes steps of projecting the source of ultraviolet light to be directed onto one or more dental composite materials on a tooth of a patient. The dental tools used for the method include a visible light source, an ultraviolet light source and a switch which switches light projected from the tool between visible light and ultraviolet light.

The disclosed apparatus, systems and methods relate to devices, systems and methods for intra-operative imaging.

Systems and methods for measuring short wave infrared fluorescence and autofluorescent signals are disclosed. In some embodiments, for example, a method may include exposing a portion of tissue that does not include a fluorescent probe to an excitation source of the tissue, wherein at least a portion of the tissue has an autofluorescence spectrum which includes wavelengths greater than 900 nm, and imaging the tissue with a detector that is sensitive to electromagnetic radiation with wavelengths greater than or equal to 900 nm. In certain other embodiments, a system comprises a fluorescent probe including a fluorescent component attached to a carrier, an excitation source, and a detector that detects a tail portion of the fluorescence of the fluorescent component. Methods associated with such a system are also disclosed.

The present invention provides the methods of synthesis of [5]helicene compounds and the use of the said compounds conjugating with biomolecules to work as molecular reporter for diagnostic. The compounds in the present invention have the chemical structure illustrated in the formula (1): wherein G is a connecting group composes of 2 carbon atoms selected from the group consisting of ethane and ethylene; A is a separated or connected group selected from the group consisting of cyano and imide; D1 is selected from the group consisting of oxyalkanoic acid, oxyalkanal and oxyalkanesulfonate; and D2 has structure selected from the group consisting of hydroxyl, oxyalkanoic acid, oxyalkanal, alkyl oxyalkanoate, oxyalkanol and oxyalkanesulfonate. The compounds in the present invention compose of aromatic [5]helicene core comprising long it-conjugating system. The said compounds contain functional groups which able to link with biomolecules and they are soluble in water or other solvents that used in binding process with biomolecules. Moreover, owing to having proper chemical structure, the compounds in the present invention exhibit good fluorescent emission in wavelength of 425-675 nm. When the said compounds connected with biomolecules, the biomolecules give good fluorescence and can be detected under ultraviolet radiation. ##STR00001##

The present disclosure relates to compounds that are useful as near-infrared fluorescence probes, wherein the compounds include i) a ligand that binds to the active site of carbonic anhydrase, ii) a dye molecule, and iii) a linker molecule that comprises an amino acid, amide, ureido, or polyethylene glycol derivative thereof. The disclosure further describes methods and compositions for making and using the compounds, methods incorporating the compounds, and kits incorporating the compounds.

An arrangement for operating a biosensor emitting radiation includes an excitation light source, which generates at least one excitation radiation for the biosensor; a coupling fiber, at the entry surface of which the excitation radiation is coupled in; an optical Y-coupler, including an excitation arm, which is connected to the exit surface of the coupling fiber, a detector arm, which is connected to an optical detector, and a sensor foot, which can be connected to the biosensor. The excitation arm has a conical shape. The radiation axis of the excitation arm includes an angle in the range of 5° to 70° with the main radiation axis of the detector arm. The diameter of the excitation arm at the connecting point to the detector arm is less than two thirds the diameter of the detector arm. An arrangement for determining the glucose content blood is also provided.

A treatment method and a treatment system capable of treating a tumor while checking a degree of destruction of tumor cells due to emission of light and improving a treatment effect. A treatment system configured to irradiate a photosensitive substance accumulated in a tumor cell of breast cancer with excitation light, and includes: an optical device including an optical fiber configured to propagate light between a proximal portion and a distal portion of the optical device, and including, at the distal portion, an irradiation unit configured to emit light outward, and a detection unit configured to detect external light. The distal portion of the optical device is configured to be inserted into a lactiferous duct from a lactiferous duct orifice.