The present disclosure relates generally to devices, systems, and methods for diagnosis and treatment via laser-treated skin and, more particularly, to devices, systems, and methods for inducing leakage or rupture of one or more blood vessels comprising the dermis for various diagnostic and therapeutic applications. Other aspects of the present disclosure can include methods for detecting one or more target analytes in a dermis of a subject, methods for facilitating skin-to-blood delivery of agent in a subject, and methods for collecting a fluid sample from the dermis of a subject.

Laser light is emitted from a laser into a scattering layer. An ultrasound signal is emitted into a sample. A signal is generated with a light detector in response to a measurement beam of laser light exiting the light scattering layer into the light detector. At least a portion of the measurement beam formed between the laser and the light detector is wavelength-shifted by the ultrasound signal subsequent to the ultrasound signal propagating through the sample.

The present invention provides a method for treating a dermatological condition in a subject, comprising administering to the subjects skin a bioactive composition using a microneedle delivery device, wherein the bioactive composition comprises an effective amount of an anesthetic; administering an effective amount of electromagnetic radiation to the subjects skin to induce damage to the epidermis and/or dermis; and optionally administering to the subjects skin an effective amount of a composition to promote wound healing.

Exemplary embodiments of a method, device, and apparatus for positioning an apparatus on a location of a tissue. For example, a substantially rigid film over an area of tissue to be treated can be provided. The film can include a positioning arrangement that facilitates a particular spatial engagement with the apparatus. The exemplary method can further include applying a portion of the film to a portion of a surface of the tissue and positioning the apparatus at the location by the engagement of the film to the apparatus using the feature of the film.

Laser light is emitted from a laser into a scattering layer. An ultrasound signal is emitted into a sample. A signal is generated with a light detector in response to a measurement beam of laser light exiting the light scattering layer into the light detector. At least a portion of the measurement beam formed between the laser and the light detector is wavelength-shifted by the ultrasound signal subsequent to the ultrasound signal propagating through the sample.

A laser irradiating device preferably includes: a reflector having a receiving space formed therein; a flash lamp inserted and mounted in the reflector to generate light; a laser rod for resonating light incident from the flash lamp to emit a laser; a capacitor for storing, for a predetermined time interval, voltage to be supplied to the flash lamp; a digital variable resistor unit for outputting different voltages according to configured resistance values; a voltage increasing unit for increasing voltage input from the digital variable resistor unit and supplying the increased voltage to the capacitor; a control unit which stores resistance values corresponding to laser irradiating levels and configures a resistance value corresponding to the configured laser irradiating level; and a trigger circuit unit turned on according to a control of the user to supply a charge voltage of the capacitor to the flash lamp.

Exemplary embodiments of a method, device, and apparatus for positioning an apparatus on a location of a tissue. For example, a substantially rigid film over an area of tissue to be treated can be provided. The film can include a positioning arrangement that facilitates a particular spatial engagement with the apparatus. The exemplary method can further include applying a portion of the film to a portion of a surface of the tissue and positioning the apparatus at the location by the engagement of the film to the apparatus using the feature of the film.

Exemplary embodiments of a method, device, and apparatus for positioning an apparatus on a location of a tissue. For example, a substantially rigid film over an area of tissue to be treated can be provided. The film can include a positioning arrangement that facilitates a particular spatial engagement with the apparatus. The exemplary method can further include applying a portion of the film to a portion of a surface of the tissue and positioning the apparatus at the location by the engagement of the film to the apparatus using the feature of the film.

A laser irradiating device preferably includes: a reflector having a receiving space formed therein; a flash lamp inserted and mounted in the reflector to generate light; a laser rod for resonating light incident from the flash lamp to emit a laser; a capacitor for storing, for a predetermined time interval, voltage to be supplied to the flash lamp; a digital variable resistor unit for outputting different voltages according to configured resistance values; a voltage increasing unit for increasing voltage input from the digital variable resistor unit and supplying the increased voltage to the capacitor; a control unit which stores resistance values corresponding to laser irradiating levels and configures a resistance value corresponding to the configured laser irradiating level; and a trigger circuit unit turned on according to a control of the user to supply a charge voltage of the capacitor to the flash lamp.

The laser lancing device in accordance with an exemplary embodiment includes: a main body; a laser resonator located within the main body and configured to generate a laser and output the laser forwards; a beam barrel located in front of the laser resonator and including at least one lens unit fixed therein; a window barrel located in front of the beam barrel and connected to the main body; a cap part connected to the front of the window barrel and brought into contact with an irradiation target area; a fan unit communicating with the cap part and induce flow of air; and a communication pipe of which one end is connected to the fan unit and the other end is connected to the cap part.