A system for preparing a harvested blood vessel for use as a graft during a coronary artery bypass procedure, which includes an elongated platform, a mounting flange extending upwardly from a top surface of the platform adjacent a proximal end thereof for supporting a syringe in a fixed position, and a pair of longitudinally spaced apart clamp holders operatively associated with the top surface of the platform, each clamp holder configured to support a respective vessel clamp, wherein the vessel clamps supported within the clamp holders are adapted to hold a harvested blood vessel immobile therebetween so it can be prepared for grafting.

A platform for creating engineered tissues includes a vascular tube that defines a vascular diameter and is configured to receive vascular system seed cells, a non vascular tube that defines a non-vascular tube diameter and is configured to receive organ system seed cells, and a barrier formed between the vascular tube and the non vascular tube.

Methods of producing hybrid fibrous scaffolds are provided. The methods include dissolving a polymer, such as polydioxanone, in a solution, such as 1,1,1,3,3,3-hexafluoro-2-propanol (HFP), to form a polymer-containing solution. The method comprises electrically charging the polymer-containing solution. The method comprises writing the polymer-containing solution on a counter electrode or a ground in a grid pattern to form semi-stable fibers comprised of the polymer, the semi-stable fibers vary between bent and straight and forming the hybrid fibrous scaffold. The writing may be performed by a 3D printer. The resulting scaffolds and methods of using the same are also disclosed herein.

The present invention provides a tissue-engineering vascular graft (TEVG) comprising a biodegradable scaffold, and a plurality of stem cell-derived vascular smooth muscle cells (VSMCs), wherein the plurality of stem cell-derived VSMCs are seeded on the biodegradable synthetic polymer scaffold and are cultured under mechanical and biochemical stimulation.

This disclosure describes decellularized, biologically-engineered tubular grafts and methods of making and using such decellularized, biologically-engineered tubular grafts.

An apparatus can include a triply periodic minimal surface. The apparatus can include a 3D scaffold formed from the triply periodic minimal surface. The apparatus can include one or more channels formed by the 3D scaffold. A method of forming a gas exchange unit can include printing a 3D scaffold formed from a triply periodic minimal surface. The 3D scaffold can include a vascular network configured to conduct a fluid. The 3D scaffold can include one or more channels configured to hold a gas. The vascular network can be embedded inside walls of the 3D scaffold. The one or more channels can be positioned between the walls of the 3D scaffold.

The invention relates to an artificial vascular graft comprising a primary scaffold structure encompassing an inner space of the artificial vascular graft, said primary scaffold structure having an inner surface facing towards said inner space and an outer surface facing away from said inner space, a coating on said inner surface, wherein a plurality of grooves is comprised in said coating of said inner surface. The primary scaffold structure comprises further a coating on said outer surface. The primary scaffold structure and the coating on said inner surface and on said outer surface are d designed in such a way that cells, in particular progenitor cells, can migrate from the periphery of said artificial vascular graft through said outer surface of said coating, said primary scaffold structure and said inner surface to said inner space, if the artificial vascular graft is used as intended. The invention relates further to a method for providing said graft.

A network (100) for replacement of a living tissue, said network is a scaffold-free artificial hollow biological tissue network comprising a plurality of longitudinal multicellular aggregates (11) arranged in a plurality of bioprinted layers (22) which are located on top of one another, further comprising an inner surface (20) and an outer surface (21), wherein at least one of said bioprinted layers (22) is in shape of a planar closed loop such that a conduit for conveying fluids is defined, and said longitudinal multicellular aggregate (11) is a mixture of at least two cell types. Also a method for obtaining said longitudinal multicellular aggregate, and a further method for biomodeling and planning said network are proposed.

An intravascular cell therapy device comprises a scaffold (2, 12) that is radially adjustable between a contracted orientation suitable for transluminal delivery to a vascular locus and an expanded orientation, and a biodegradable matrix provided on at least a portion of the scaffold that is suitable for seeding with cells and degrades in a vascular environment. The scaffold is configured to have a distal piercing tip (5) when in a deployed orientation. The scaffold comprises a plurality of sidewall panels (3, 13, 14) arranged around a longitudinal axis of the scaffold, and adjustable couplings (4) between the panels configured for adjustment between an expanded configuration and a contracted orientation, and in which each sidewall panel comprises a matrix suitable for seeding with cells.

Apparatus, systems, and methods for endoscopic dissection of blood vessels and control over cavity pressure within an endoscopic procedure are described herein. Apparatus, systems, and methods for harvesting of blood vessels are also described herein.