Device and method for implantation of hair grafts into the scalp of a patient. In the device, a cross-sectional shape of a rod riding in an implantation needle is configured with respect to the needle to communicate a vacuum in a chamber anterior to the needle forward to an interior of the needle, thereby facilitating loading of the hair graft into the device by direct-to-needle aspiration of the hair graft.

Device and method for implantation of hair grafts into the scalp of a patient. In the device, a cross-sectional shape of a rod riding in an implantation needle is configured with respect to the needle to communicate a vacuum in a chamber anterior to the needle forward to an interior of the needle, thereby facilitating loading of the hair graft into the device by direct-to-needle aspiration of the hair graft.

A non-invasive method and system for assessing the survival of a transplanted flap involve the following steps. A control unit instructs a variable-frequency current generator circuit to generate a constant current at a fixed frequency and pass the constant current through a detection electrode. The detection electrode detects a bioelectrical impedance of the skin to which the flap has been transplanted, and the bioelectrical impedance of the skin is compared with a pre-defined threshold value. If the bioelectrical impedance of the skin exceeds the pre-defined threshold value, it is determined that an abnormal condition has occurred.

A density gradient biopolymeric matrix implant is disclosed. The implant includes a first homogeneous matrix layer and a second homogeneous matrix layer having a density different from that of the first homogeneous matrix layer. Biopolymeric fibers at the surface of the first homogeneous matrix layer are physically in contact with and cross-linked to the biopolymeric fibers at the surface of the second homogeneous matrix layer. Also disclosed is a three-dimensional density gradient biopolymeric matrix implant that includes a first homogeneous matrix surrounding a second homogeneous matrix having a different density. Biopolymeric fibers at an inner surface of the first homogeneous matrix are physically in contact with and cross-linked to biopolymeric fibers at an outer surface of the second homogeneous matrix. Furthermore, methods for preparing the density gradient biopolymeric matrix implant and the three-dimensional density gradient biopolymeric matrix implant are provided.

A method includes forming a scaffold and seeding the scaffold with live cells; growing the cells in the scaffold; and 3D printing the cells into a living subject, where the cells continue to live in the living subject.

A hair follicle implant device includes a needle holding assembly configured to hold a plurality of needles and a needle guide slidably coupled thereto and configured to provide a skin stop surface while guiding the plurality needles. A plurality of pistons is configured to slide within a respective one of the plurality of needles. A piston base is slidably coupled to the needle holding assembly and is configured to hold the pistons to slide within the plurality of needles when actuated during implantation. A spring, carried between the needle holding assembly and the piston base, is configured to bias the piston base in a retracted position, wherein the spring is offset from a central axis of the piston base. The needle holder holds the plurality of needles in the staggered arrangement offset from the central axis of the piston base.

A hair follicle implant device includes a needle holding assembly configured to hold a plurality of needles and a needle guide slidably coupled thereto and configured to provide a skin stop surface while guiding the plurality needles. A plurality of pistons is configured to slide within a respective one of the plurality of needles. A piston base is slidably coupled to the needle holding assembly and is configured to hold the pistons to slide within the plurality of needles when actuated during implantation. A spring, carried between the needle holding assembly and the piston base, is configured to bias the piston base in a retracted position, wherein the spring is offset from a central axis of the piston base. The needle holder holds the plurality of needles in the staggered arrangement offset from the central axis of the piston base.

The present invention provides an artificial skin and a preparation method thereof. The present invention takes the xenogeneic acellular dermal matrix particles as main materials, and obtains the dermis layer by three-dimensional printing technologies, and then obtains the artificial skin by combining the epidermis layer with the dermis layer. The dermis layer of artificial skin in present invention has three-dimensional porous structure, which retains main components of natural dermal matrix in composition, and imitates distributed structure at fiber bundle diameter and pore size of natural dermal matrix in structure. This kind of novel biomimetic dermal scaffolds have obvious advantages in inducing migration and regeneration of skin cells, accelerating vascularization, promoting wound healing and improving healing quality. The dermis layer of artificial skin in present invention is obtained by three-dimensional printing technologies, which has precise and controllable structure, simple preparation method and high products qualification rate.

The present specification discloses a method capable of automatically recognizing an accurate wound boundary and a method of generating a 3D wound model based on the recognized wound boundary. The method of automatically recognizing a wound boundary according to the present specification is a method of automatically recognizing a wound boundary based on artificial intelligence, and may photograph several frames of the wound to be recognized with an RGB-D camera, separating measurement information in an image, amplifying image data for learning, and passing the amplified image data through an artificial neural network. The method may include generating a three-dimensional (3D) model by performing boundary recognition post-processing on the data passing through the artificial neural network to match a two-dimensional (2D) image with the 3D model.

A bio-printing process comprises a step of preparing a target digital model representative of the three-dimensional organization of the tissue to be produced, a step of controlling a bio-printing instrument for the deposition of a plurality of layers of living cells and of biomaterials, a step of calculation of a digital printing model as a function of the digital model of the product to be produced, and of a model predicting change, and also characteristics of the constituents to be printed. The step of controlling the bio-printing instrument is carried out according to the digital printing model calculated in this way. A system is also described for implementing this process.