The present invention provides a composition comprising fetal cartilage tissue-derived cells and a fetal cartilage tissue-derived extracellular matrix as active ingredients for regenerating a growth plate. The composition for regenerating a growth plate can inhibit bone bridge formation in a growth plate injury region without a scaffold and differentiate to a growth plate cartilage tissue to effectively fill and regenerate the injured region therewith, whereby the regenerated growth plate tissue can recover growth ability. In addition, the composition is compatible with and safe to biological tissues and is characterized by high reproducibility and homogeneity.

A composition, for inducing chondrocyte differentiation or regenerating cartilage tissue or both, includes exosomes derived from stem cells differentiating into chondrocytes.

A composition, for inducing chondrocyte differentiation or regenerating cartilage tissue or both, includes exosomes derived from stem cells differentiating into chondrocytes.

The present invention is directed to a method of preparing an artificial tooth primordium in vitro, comprising the steps: a) providing isolated mesenchymal dental pulp cells; and b) culturing the mesenchymal dental pulp cells under non-adherent conditions to form a cell aggregate representing an artificial tooth primordium; as well as to an artificial tooth primordium derived therefrom.

The present invention is directed to a method of preparing an artificial tooth primordium in vitro, comprising the steps: a) providing isolated mesenchymal dental pulp cells; and b) culturing the mesenchymal dental pulp cells under non-adherent conditions to form a cell aggregate representing an artificial tooth primordium; as well as to an artificial tooth primordium derived therefrom.

A method of producing an osteoinductive composition for in vivo use, in which the method includes obtaining bone tissue; cleaning the bone tissue with a plurality of washes; milling, grinding, and/or cutting the cleaned bone tissue into a plurality of bone pieces or fibers of a desired size; and demineralizing the plurality of bone pieces or fibers of a desired size to expose one or more native bone proteins, with demineralizing including combining the plurality of bone pieces or fibers in a reaction vessel with an ionic acid solution, soaking the plurality of bone pieces or fibers in the ionic acid solution, and exposing the one or more native proteins in the bone pieces or fibers.

A method of producing an osteoinductive composition for in vivo use, in which the method includes obtaining bone tissue; cleaning the bone tissue with a plurality of washes; milling, grinding, and/or cutting the cleaned bone tissue into a plurality of bone pieces or fibers of a desired size; and demineralizing the plurality of bone pieces or fibers of a desired size to expose one or more native bone proteins, with demineralizing including combining the plurality of bone pieces or fibers in a reaction vessel with an ionic acid solution, soaking the plurality of bone pieces or fibers in the ionic acid solution, and exposing the one or more native proteins in the bone pieces or fibers.

Disclosed is a cosmetic composition for skin soothing. The cosmetic composition includes, as active ingredients, exosomes derived from naturally occurring deer antler velvet. The cosmetic composition can effectively soothe the skin whose condition has been abnormally altered (such as atopic dermatitis, inflammation, erythema, oxidation, cytotoxic agents in the skin, loss of moisture, melasma, itching, roughness or wrinkles) by various causes.

A mesenchymal stem cell harvesting system and method for increasing the efficiency of collecting and processing physiological fluids containing mesenchymal stem cells from a cavity within a patient’s skeletal system. Microenvironments risk in MSC production and concentration within a cavity, for example the patient’s ilium, are penetrated with a pointed instrument used to create an aperture in the hard cortical bone forming the cavity followed by the insertion of an aspiration device which extracts one or more samples of cancellous bone, bone marrow, bone marrow blood and other aspirated material. The aspirate is rinsed and may be filtered to remove unwanted material and to increase the concentration and purity of the mesenchymal stem cells in the aspirant far beyond levels formerly obtainable for use in autologous treatment of the patient.

The present invention relates to an herbal composition in powder, pill or liquid form for speeding up fracture healing and bone regeneration, which may promote fracture healing and bone regeneration by containing medicinal herbs capable of improving fracture healing and bone regeneration and medicinal herbs capable of enhancing digestion and absorption, and an herbal formulation in powder, pill or liquid form containing the same. The herbal composition in powder, pill or liquid form for speeding up fracture healing and bone regeneration according to the present invention contains 20 parts by weight of Angelica gigas, 16 parts by weight of Dendrobium moniliforme, 12 parts by weight of Dipsaci radix, 10 parts by weight of antler, 8 parts by weight of Cnidium officinale, 8 parts by weight of Astragalus membranaceus, 4 parts by weight of Achyranthes japonica, and 4 parts by weight of Massa medicata fermentata.