Provided are a composition for preventing hair loss and stimulating hair growth, which includes small-sized stem cells, particularly, small-sized stem cells having a diameter of 8 μm or less, or a culture thereof as an active ingredient, a method of preparing the same, and a use of the same. Provided is a use of the composition utilizing a hair growth stimulating function of the small-sized stem cells having a diameter of 8 μm or less to considerably increase activity of hair follicle stem cells in a telogen phase.

The invention refers to a decomposed platelet concentrate containing ≤50% intact platelets and optionally ≥200 pg/ml RANTES and specifically to its use in the prevention or treatment of hair loss and/or for promoting hair growth. The invention further refers to a composition comprising the decomposed platelet concentrate and body fat cells and the use of said composition for reconstructive and aesthetic and curative surgery and pain relief treatment.

Formulations and methods for activating signaling molecules outside a native physiological response are provided. The formulations can include an activator component and a signaling component containing both signaling molecules and mesenchymal stem cells. When a formulation containing both an activator component and a signaling component is injected into or applied to a treatment area, the activator component may activate the signaling molecules of the signaling component which in turn signal the mesenchymal stem cells of the signaling component to regrow, repair or otherwise provide a benefit to the treatment area.

Use of an inducible extracellular vesicle as a medicament for skin, health care products or skin care products. A composition, comprising the inducible vesicle and the medicaments or care products for skin.

Apparatus and methods for separating blood components are disclosed in which an apparatus for separating blood generally includes a tube defining a channel and configured for receiving a quantity of blood and a float contained within the tube and having a density which is predefined so that the float is maintained at equilibrium between a first layer formed from a first fractional component of the blood and a second layer formed from a second fractional component of the blood. Upon completion of the centrifugation, the first layer may be removed from the tube while the float isolates the second layer from the first layer.

The present invention relates to a composition for hair regeneration comprising a macrophage-derived extracellular vesicle mimetics, and since macrophage-derived extracellular vesicle mimetics are well absorbed into dermal papilla cells, have an excellent effect in promoting the proliferation of dermal papilla cells, as well as having an activity to increase the level of hair-induced proteins, it is excellent in the effects of proliferation of dermal papilla cells and hair increase and thus a composition comprising macrophage-derived extracellular vesicle mimetics can be provided for hair regeneration.

There is provided a formulation for skin rejuvenation comprising lyophilised umbilical cord plasma comprising at least one active chemokine, at least one growth factor and at least one cytokine; a transdermal carrier; and a liposomal base, wherein at least a portion of the lyophilised umbilical cord plasma and transdermal carrier are contained within liposomes of the liposomal base. There is also provided a dosage form for skin rejuvenation comprising 1-5% (w/w) lyophilised umbilical cord plasma comprising at least one active chemokine, at least one growth factor and at least one cytokine; 85-95% (w/w) polyethylene glycol; and 0.1-2% (w/w) gum acacia.

Disclosed herein are aesthetic-enhancing formulations and methods thereof. For example, an aesthetic-enhancing formulation includes, in some embodiments, one or more pigments, one or more active ingredients, and one or more excipients. The one-or-more excipients includes at least a vehicle configured to evenly distribute components throughout the aesthetic-enhancing formulation, the components including the one-or-more pigments, the one-or-more active ingredients, and any other excipients other than the vehicle. In another example, a method for the aesthetic-enhancing formulation includes, in some embodiments, inserting the aesthetic-enhancing formulation into a dermal layer of skin by percutaneous punctures.

The present disclosure relates to the cosmetology field, in particular to an activated revitalizing lotion and its preparation method and application. A preparation method of an activated revitalizing lotion comprises the steps of: mixing an isolated blood sample, ozone-oxygen mixture and sodium citrate, and centrifuging the mixed blood sample at 1800-2000 r/min for 9-10 min to obtain a plasma supernatant for use as an activated revitalizing lotion; the ozone-oxygen mixture has an ozone concentration of 42.8-171.4 μg/L and added in an amount of 2.3-3.2 L/1 L of the isolated blood sample; and sodium citrate is added in an amount of 0.2-0.3 mg/1 L of the isolated blood sample. The activated revitalizing lotion prepared by the preparation method of the present disclosure shows improved effect in repair and revitalization of skin or subcutaneous tissues.

The present invention relates to a method for preparing a culture medium containing exosomes released by umbilical cord blood stem cells. The preparation method is based on culturing the umbilical cord blood stem cells in a serum-free medium supplemented with GDF-11, EGF, FGF2, TFG-β1, and/or VEGF. The culture medium produced by treating umbilical cord blood stem cells with a serum-free medium supplemented with 5 types of growth factors and the exosomes isolated from the culture medium has a higher total protein content and extracellular matrix protein content, when compared to a culture medium produced by culturing umbilical cord blood stem cells in a serum-free medium to which the growth factor is not added. In addition, exosomes released by umbilical cord blood stem cells cultured in a serum-free medium containing five growth factors have a high concentration, a small size, and an even distribution.

The umbilical cord blood stem cell culture medium prepared according to the present invention contains a high content of high-potency exosomes and has a high skin regeneration and/or anti-inflammatory effect, so it can be used in a cosmetic composition for skin application requiring skin penetration and a pharmaceutical composition for wound treatment.