A nanobowl-supported drug-loaded liposome, a preparation method therefor, and an application thereof. The preparation method for the nanobowl-supported drug-loaded liposome comprises: incubating and ultrasonically treating a nanobowl and a liposome and then successfully encapsulating a drug by utilizing an ammonium sulfate active drug loading method to obtain the nanobowl-supported drug-loaded liposome. The nanobowl-supported drug-loaded liposome can resist the influence of plasma proteins and blood flow shearing forces on drug leakage, enhance the delivery of the drug at a tumor site, improve carrier stability, and improve an anti-tumor curative effect.

Described herein are ultra-deformable liposomes comprising a first lipid and second lipid. The first lipid comprises a first hydrophilic head linked to a first aliphatic tail, and the second lipid comprises a second hydrophilic head linked to a second aliphatic tail having at least two carbons less than the first aliphatic tail. The ultra-deformable liposomes described herein are useful, for example, as drug delivery vehicles. Accordingly, also described herein are compositions comprising an ultra-deformable liposome and a cargo, such as a drug, as well as methods for delivering a drug, such as an anti-cancer therapeutic, to a tumor.

The compounds disclosed herein (e.g., compounds having a structure according to Formula (I), (II), (III), (IV), and (V)) are polymers wherein an organic polymeric segment (e.g., a polyethylene glycol (PEG) group) comprises covalent attachments to two or more lipid substructures, and each lipid substructure independently comprises a hydrophobic moiety and a hydrophilic moiety. The compounds provided herein can be useful for delivery and expression of mRNA and encoded protein, e.g., as a component of liposomal delivery vehicle, and accordingly can be useful for treating various diseases, disorders and conditions, such as those associated with deficiency of one or more proteins.

The present invention provides polymersomes comprising amphiphilic block-copolymers and their use to quantify ammonia in samples (e.g., body fluid samples). More particularly, it provides a polymersome comprising (a) a membrane, which comprises a block copolymer of poly(styrene) (PS) and poly(ethylene oxide) (PEO), wherein the PS/PEO molecular weight ratio is higher than 1.0 and lower than 4.0; and (b) a core which encloses an acid and at least one pH-sensitive dye. Compositions, strips and kits comprising the polymersomes are also provided along with methods of quantifying ammonia in a sample using the polymersomes, compositions and kit.

Disclosed are cationic lipids which are compounds of Formula I. Cationic lipids provided herein can be useful for delivery and expression of mRNA and encoded protein, e.g., as a component of liposomal delivery vehicle, and accordingly can be useful for treating various diseases, disorders and conditions, such as those associated with deficiency of one or more proteins. ##STR00001##

The present disclosure provides novel modified nanodroplets with a layerby-layer (LBL) assembly formulation (“LBLnNDs”). The LBLnNDs of the present disclosure comprise gaseous perfluorocarbon core, polymer shell, and multiple alternating positively and negatively charged biopolymer layers dispersed layerby-layer onto the shell of the LBLnNDs. Methods of making and use of the LBLnNDs are also provided.

The present invention relates to cell delivery systems. The present invention specifically relates to new methods of intracellular delivery by endocytosis routing sequence peptides having the sequence of WYKYV or analogues thereof, by caveloar/lipid raft-mediated endocytosis and uses of such peptides. The invention also relates to conjugates comprising said peptides and uses thereof in therapies wherein intracellular delivery of a therapeutically active molecule is required.

[Problem to be Solved]

Provided is a delivery carrier into the cell having high antioxidant activity, intracellular absorbability, intracellular disintegration property, stability and safety, which have high delivery property of the active ingredient to cell and living tissues.

[Solution]

The delivery carrier into cells includes a vitamin derivative with co-activation of both autophagy-related genes and protease synthesis genes, a polymer molecule containing stimulatory reactivity, an emulsion stabilizer, an active ingredient, and a lipid, thereby providing a delivery carrier into cells with high delivery properties of the active ingredient to cells and living tissues.

Provided herein are block copolymers comprising a hydrophilic polymer segment and a hydrophobic polymer segment, wherein the hydrophilic polymer segment comprises a polymer selected from the group consisting of: poly(ethylene oxide) (PEO), poly(methacrylate phosphatidyl choline) (MPC), and polyvinylpyrrolidone (PVP), wherein the hydrophobic polymer segment comprises

##STR00001##

wherein R′ is —H or —CH.sub.3, wherein R is —NR.sup.1R.sup.2, wherein R.sup.1 and R.sup.2 are alkyl groups, wherein R.sup.1 and R.sup.2 are the same or different, wherein R.sup.1 and R.sup.2 together have from 5 to 16 carbons, wherein R.sup.1 and R.sup.2 may optionally join to form a ring, wherein n is 1 to about 10, and wherein x is about 20 to about 200 in total. Also provided are pH-sensitive micelle compositions for therapeutic and diagnostic applications.

The present invention concerns amphiphilic copolymers that may be photo- or redox-cleavable and that may assemble into polymersomes. It also concerns their process of preparation and their use as ding carriers.