An improved wound honey using a combination of buckwheat honey, honeydew honey, a natural thickening agent of blackstrap molasses and tangerine puree, and fragrance comprising lavender essential oil. The wound honey is sterilized using a flowing UV light sanitizing system. The bottles are disinfected using a combination parecetic acid, formic acid, vanillin, clove essential oil, monolaurin, aloe vera, and hydrogen peroxide disinfectant. The product comes in a container with a pump dispenser that dispenses a quantifiable amount of product. This new wound honey with pump dispenser may be used multiple times, is cheaper than manuka honey, and may be produced outside of New Zealand and Australia.

The present disclosure provides a bioprinting system (100) for printing a liquid directly onto a subject. The bioprinting system (100) comprises a bioprinting assembly (102). Optionally, a robotic arm (104) and a control system (150) are provided. The bioprinting assembly (102) may be coupled to the robotic arm (104) to be positionable relative to the subject. The bioprinting assembly (102) is configured to dispense the liquid onto the subject and comprises a reservoir (120) for holding the liquid and a loading mechanism (134) to prime the reservoir (120) with the liquid directly prior to printing. The loading mechanism (134) has a one way inlet to permit liquid to be loaded into the reservoir (120) and prevent fluid from exiting the reservoir via the one way inlet. There is also provided associated methods.

Disclosed are methods of promoting wound healing in a patient in need thereof comprising administering to the patient a composition comprising a neonatal fibrin scaffold. Further disclosed are in vitro methods for evaluating a target composition on human wound healing comprising a neonatal porcine plasma scaffold with the target composition and evaluating scaffold properties of the plasma sample.

The present disclosure provides a wound dressing assembly comprising (i) a blood-clotting mold device with a mold cavity for forming a molded blood clot for use in dressing a wound. Prior to extraction, the molded blood clot is pushed towards the mold cavity's interior, which causes the molded blood clot to disassociate from the cavity walls and thereafter it can be removed with none or only minimal damage to its integrity.

Wound dressing assembly including (i) blood-clotting mold device having an enclosure defined between walls of a main body and a removable closure over an opening and configured for introduction of blood thereinto, and (ii) coagulation initiator in amount sufficient to coagulate blood introduced into the enclosure to form a blood clot. The formed blood clot is transferable onto a wound. Method for preparing a wound dressing by introducing a volume of blood into the enclosure of the blood-clotting mold device, maintaining the blood within the enclosure for time sufficient to permit clotting of the blood to obtain a blood clot; removing the closure to open the enclosure; and extracting the blood clot from the enclosure. The formed blood clot may be used in a method for dressing a wound by fixation of the clot onto the wound.

Provided are methods, devices and compositions for reducing and/or inhibiting postsurgical tissue adhesion using a hydrogel film disposed onto a target tissue, thereby providing an adhesion barrier that remains over said target tissue for a prescribed period of time. In some embodiments, the hydrogel film is formed by the gelation of a pre-gel mixture applied onto the target tissue as a plurality of particles having an average maximum dimension, such as diameter, of at most about 500 μm. In some embodiments, the hydrogel film has a minimum storage modulus of 100 Pa. In some embodiments, the pre-gel mixture comprises an ECM digest having a collagen to carbohydrate ratio (by mass) of at least 70:1.

In at least one embodiment there is a wound application comprising at least one film applied over a wound comprising at least 1% green tea, and at least 0.01% sugar, wherein said film comprises a polymeric film comprising united chains and monomeric glucose points. In at least one other embodiment there is a wound application comprising a solution comprising at least trace amounts of colloidal silver, at least trace amounts of sodium chloride, at least trace amounts of dextrose; and water, wherein the colloidal silver, the sodium chloride and the dextrose are dissolved in water. In at least one other embodiment there is an application for treating a wound comprising both a solution comprising at least trace amounts of colloidal silver and a film comprising at least trace amounts of green tea applied over the solution on top of a wound.

Natural honey and a surfactant are combined to achieve a novel wound treatment composition. The honey is chosen for its medicinal properties, and the surfactant is used for biofilm removal to enhance the effectiveness of the honey. In preferred embodiments, the honey is a tropical honey currently sourced from Mexico. The surfactant is mixed with water, then the aqueous mixture is combined with the honey. In accordance with a preferred embodiment, a surfactant powder is mixed with water at a ratio of 10% powder to water, then that mixture is combined with honey at a ratio greater than 50:50, more preferably at a ratio of 90:10. The currently preferred surfactant is Poloxamer 188. The product may be applied directly to a wound or applied to wound dressings including, without limitation, gauze, gauze rolls, hydrocolloids, and calcium alginate dressings.

The present invention relates to methods for adhering tissue surfaces and materials and biomedical uses thereof. In particular the present invention relates to a method for adhering a first tissue surface to a second tissue surface in a subject in need thereof, comprising the steps of adsorbing a layer of nanoparticles on at least one of the tissue surfaces, and approximating the surfaces for a time sufficient for allowing the surfaces to adhere to each other. The present invention also relates to a method for adhering a material to a biological tissue in a subject in need thereof, comprising the steps of adsorbing a layer of nanoparticles on the surface of the material and/or the biological tissue and approximating the material and the biological tissue for a time sufficient for allowing the material and the biological tissue to adhere to each other.

Interpenetrating network hydrogels with independently tunable stiffness enhance tissue regeneration and wound healing.