The present invention relates to a decellularized nerve graft using allogeneic and heterologous nervous tissues and a method of manufacturing the same.

In the present invention, by using a low-concentration basic solution and a surfactant as a decellularization solution, cell and tissue toxicity caused by a solvent or surfactant remaining in the tissue may be minimized by minimizing the use of a basic solution and an anionic surfactant in the entire manufacturing process. In addition, a peristaltic pump may be used to maintain the tissue structure and effectively remove lipid and cells.

A method of treating a spinal cord injury in a subject in need thereof is disclosed. The method comprises implanting a scaffold into the spinal cord of a subject, wherein the scaffold is seeded with oral mucosa stem cells (OMSC) and/or cells that have been ex vivo differentiated from said OMSCs, thereby treating the spinal cord injury.

Methods of treating a complex wound by administering to a complex wound in the individual a therapeutically effective amount of a fetal support tissue product to treat the complex wound. Methods of treating a complex lower extremity ulcer by administering to a complex lower extremity ulcer in the individual a therapeutically effective amount of a fetal support tissue product to treat the complex lower extremity ulcer. Methods of reducing or preventing scar formation from granulation tissue by administering a fetal support tissue product to granulation tissue. Methods of repairing a spina bifida defect by administering to the defect in the individual a therapeutically effective amount of an umbilical cord product.

A method of producing an acellular peripheral nerve graft comprises the steps of providing a section of peripheral nerve, primary treatment of the section of peripheral nerve comprising freezing and then thawing the section of peripheral nerve, freeze-drying the thawed section of peripheral nerve longitudinally to introduce longitudinal pores into the section of peripheral nerve, and decellularization of the section of peripheral nerve comprising contacting the freeze-dried section of peripheral nerve with detergent and enzymatic decellularization agents to provide the acellular peripheral nerve graft. 9. The acellular peripheral nerve graft typically has an average pore size of at least 40 μm and a DNA content of less than 100 ng/mg.

Provided is a method for freezing a cell aggregate including neural cells. provided is a method for freezing a cell aggregate including neural cells and having a three-dimensional structure, which comprises following steps (1) and (2): (1) contacting a cell aggregate including neural cells and having a three-dimensional structure with a preservation solution at 0° C. to 30° C. prior to freezing to prepare a preservation solution-soaked cell aggregate; and (2) cooling the preservation solution-soaked cell aggregate obtained in step (1) from a temperature at least about 5° C. higher than the freezing point of the preservation solution to a temperature about 5° C. lower than the freezing point at an average cooling speed of 2 to 7° C./min to freeze the cell aggregate.

In various aspects and embodiments, the present invention provides methods for maintaining motor neuron health in the spinal cord and pro-regenerative capacity of a proximal nerve segment subsequent to a nerve injury in a subject in need thereof, the methods comprising transplanting a stretch-grown tissue engineered nerve graft (TENG) into a proximal site contacting the proximal nerve segment.

Systems, devices, and methods for treating a nerve injury in a patient are provided. The system includes an extracellular matrix, a neutralizing element, and a reconstituting element. The extracellular matrix is configured to promote and/or sustain the growth of tissue and/or associated tissue properties proximate the nerve injury.

The present invention relates to a method for preparing of a nerve conduit using bio-printing technology and a nerve conduit prepared by the same, and it can easily prepare a nerve conduit by simulating a nerve bundle and nerve tissue, and the like, by three-dimensionally printing bio-ink comprising a neuronal regeneration material on one side of a porous polymer scaffold.

The invention relates to a nanofibrous material comprising a drug for treating a peripheral nerve injury by delivering the drug locally to a damaged or injured nerve. The drug may be such a Non Steroidal Anti Inflammatory Drug or a PPAR agonist. In particular, the invention relates to a drug eluting nerve wrap or bandage that can be wrapped around an injured peripheral nerve. The invention also relates to a nanofibrous drug delivery system or device for delivering a drug locally to a peripheral nerve, a treatment for a peripheral nerve injury comprising contacting a damaged nerve with the drug eluting nanofibrous material or drug delivery system, kits and methods for making the nanofibrous materials, and uses of the nanofibrous materials.

The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.