The present invention relates to an in vitro method for creating a viable connective tissue and/or osseous tissue obtained by tribological solicitations of a biological culture. It further relates to a viable connective tissue and/or osseous tissue susceptible to be obtained by said method as well as to the use of said method or viable connective tissue and/or osseous tissue to prepare a biological implant.

A composition including, (a) a mineral particle, (b) endothelial cells and mesenchymal cells, and (3) hyaluronic acid, is provided. Moreover, a kit which includes: a syringe, a mineral particle covered with endothelial cells and mesenchymal cells organized in 2 or more cell layers attached to the mineral particle, and hyaluronic acid, is also provided. Last, a method for filling a gap in a bone of a subject in need thereof, including contacting the gap with a composition of: (a) a mineral particle, (b) endothelial cells and mesenchymal cells, and (3) hyaluronic acid is provided.

Provided herein is a 3D printing system and related compositions, and method of using such, that can produce a polymeric microfiber having embedded microspheres encapsulating an active agent with micron precision and high spatial and temporal resolution.

The present invention relates to porous composite materials and objects such as 3D scaffolds, in particular to bioactive and bioresorbable scaffolds that can be transformed at body temperature.

The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).

The invention relates to a porous osteoinductive calcium phosphate material having an average grain size in a range of 0.1-1.50 μm, having a porosity consisting essentially only of micropores in a size range of 0.1-1.50 μm, and having a surface area percentage of micropores in a range of 10-40%.

The present invention relates to a method for manufacturing a support for regenerating core-shell structured hard tissue and a support for regenerating core-shell structured hard tissue manufactured thereby, wherein the support may further comprise bio-functional materials, such as cells, in a core-shell structure. The method for manufacturing a support for regenerating core-shell structured hard tissue according to the present invention has an effect of manufacturing a support for regenerating core-shell structured hard tissue by a method by which a 3-dimensional structure is prepared by a layer manufacturing process through an extrusion container having a double nozzle. In addition, the support can be manufactured at room temperature, thereby having an effect of containing cells or various bio-functional materials. Furthermore, the support for regenerating core-shell structured hard tissue has a similar constitution to a bone component and thus has higher mechanical properties, and has an effect that the cells or various bio-functional materials are uniformly distributed throughout the entire 3-dimensional structure.

The present invention provides a method of bone regeneration for repairing a bone defect in a subject in need thereof. The method comprises the use of blood aspirate of the mandible bone marrow with the use of xenogen bone support.

This invention relates to a bone regeneration product comprising at least one stem cell, at least one scaffold, and at least one stem cell. The stem cells suitable for this invention may comprise stem cells suitable for a dense bone regeneration, stem cells suitable for a spongy bone regeneration, or a combination thereof. The bone regeneration product may further comprise a growth factor. This invention also relates to a bone regeneration method and treatment of any bone that has a critical size defect. This invention also relates to a scaffold. This invention further relates to a 3D printed scaffold comprising hydroxyapatite (HA) and tricalcium phosphate (TCP). This invention also relates to a scaffold comprising a polymer. The polymer of this invention may be prepared by using photocurable polymers and/or monomers. The scaffold of this invention may comprise a growth factor and a small molecule. The small molecule N may be a Smurf1 inhibitor.

Embodiments disclosed herein relate scaffolds containing fluoridated apatites sintered at a temperature of at least 950° C. and with at least one integration aid to increase integration of the scaffold in a patient, as well as methods of making and using the same.