A centrifuge is configured to provide integrated separation of blood components such that the separated products remain spinning within the centrifuge during the separation process. The centrifuge includes a disposable configured to separate the blood components such that the separated products remain within the disposable while the centrifuge is spinning; an integrated sensor system capable of determining a composition of the separated products within the disposable while the centrifuge is spinning; a chamber having a non-circular section that is configured to be deliberately un-balanced when the centrifuge chamber is empty; and the disposable includes valves that rotate with the centrifuge chamber.

A method is provided for removing red blood cells from a suspension comprising red blood cells, white blood cells, platelets and plasma using a spinning membrane separator. The method comprises: a) flowing whole blood into the gap of the spinning membrane separator; b) collecting red blood cells and white blood cells in the gap and passing plasma and platelets through the membrane; c) introducing a first quantity of lysing buffer into the gap; d) incubating the red blood cells, white blood cells and lysing buffer in the gap for a period of time to cause a lysis reaction with the red blood cells; e) introducing a second quantity of lysing buffer into the gap to displace the first quantity of lysing buffer and a first quantity of red blood cell debris out of the gap; f) introducing a first quantity of wash buffer into the gap to quench the lysis reaction and displace the second quantity of lysing buffer and a second quantity of red blood cell debris out of the gap; and g) introducing a second quantity of wash buffer into the gap to flow washed white blood cells out of the housing.

Method and Apparatus for predicting the volume of a component separated from a composite fluid by predicting the volume of the composite fluid from sensed pressure and predicting the volume of other separated components from sensed movement of the other components to collection bags.

A device for separating and recovering blood fractions includes two conical chambers communicating via their bases, the first chamber having a duct for supplying the fluid to be treated and means for recovering at least one component, the second chamber comprising an elastically deformable flexible membrane that transversely separates a space opening into the duct for communicating with the second chamber, and a bag having a volume that varies according to the deformation of the membrane.

A process for the sequential processing of opaque and transparent biological fluids such as whole blood, apheresis blood, bone marrow blood, umbilical cord blood, buffy coat or cultured cells by processing steps in a hollow cylindrical centrifugal processing chamber (300) which is part of a disposable set. At least three different procedures selected from washing, incubation, transduction, separation, density gradient separation, dilution and volume adjustment are each carried out once or repeated a number of times according to a given processing profile in the processing chamber. Each procedure involves an input into the processing chamber, an operation in the processing chamber and an output from the processing chamber by displacement of a piston (310). The at least three different procedures are sequentially chained one after the other to constitute an overall sequential operation in the processing chamber and its disposable set. A first application is incubation for binding magnetic beads with human blood cells or stem cells. A second application is transduction by which foreign genetic material is inserted into human blood cells or stem cells by a virus. A third application is reconditioning biological fluids to achieve reproducible concentration and volumes of blood cells or stem cells.

Centrifuges are useful to, among other things, remove red blood cells from whole blood and retain platelets and other factors in a reduced volume of plasma. Platelet rich plasma (PRP) and or platelet poor plasma (PPP) can be obtained rapidly and is ready for immediate injection into the host. Embodiments may include valves, operated manually or automatically, to open ports that discharge the excess red blood cells and the excess plasma into separate receivers while retaining the platelets and other factors in the centrifuge chamber. High speeds used allow simple and small embodiments to be used at the patient's side during surgical procedures. The embodiments can also be used for the separation of liquids or slurries in other fields such as, for example, the separation of pigments or lubricants.

According to some embodiments, a system may treat blood outside the body of a patient. The system may include one or more pumps configured to pump blood in a fluid flow path at a collective rate over 4 liters per minute. The system may include one or more heat exchangers operable to heat at least a portion of the blood to a temperature of at least 42 degrees Celsius and to allow the blood to cool one or more degrees following heating. The system may include one or more albumin dialysis modules configured to perform albumin dialysis on at least a portion of the blood at least after the one or more heat exchangers allow the blood to cool one or more degrees.

A fluid separation system and method includes a durable hardware component including a pump station with plurality of pumps, a centrifuge mounting station and drive unit, a plurality of valves and clamps, and a controller. The system includes a single use fluid flow circuit having a separation chamber configured to be received by the centrifuge and the fluid flow circuit is engageable with the durable hardware component to control fluid flow within the fluid flow circuit. The fluid flow circuit having an air access component configured to selectively receive air and to provide the air into a conduit to induce plug flow between the separated fluid component and another separated fluid component, wherein the controller is configured to operate the system to perform one or more blood processing procedures to convey a fluid into the separation chamber and to remove a separated fluid component from the separation chamber.

Disclosed is a bioprocessing system comprising apparatus (200) including a centrifugal separation housing (210) having a temperature controllable compartment (215) for removably accepting a separation chamber (50), the apparatus further comprising at least one mixing station (250) for supporting one or more fluid storage vessels (10, 20, 30, 40), the station including a temperature controllable area (252) for increasing or decreasing the temperature of the contents of the or each supported vessel. The system further includes a disposable fluidic arrangement (100) including a centrifugal separation chamber (50) removably mountable within the compartment (215) and having one or more ports (52) allowing fluid ingress into, or egress out of the chamber, via the one or more ports in use, said ports being in fluid communication with one or more of said fluid storage vessels via fluid conduits (12, 22, 32, 42) and via one or more valve arrangement.

A centrifuge is configured to provide integrated separation of blood components such that the separated products remain spinning within the centrifuge during the separation process. The centrifuge includes a disposable configured to separate the blood components such that the separated products remain within the disposable while the centrifuge is spinning; an integrated sensor system capable of determining a composition of the separated products within the disposable while the centrifuge is spinning; a chamber having a non-circular section that is configured to be deliberately un-balanced when the centrifuge chamber is empty; and the disposable includes valves that rotate with the centrifuge chamber.