A method and system for processing an adipose tissue material sample to create and collect fat aspirate particles having particle diameters less than or equal to a selected size are disclosed. A filter screen assembly having an interface part, a closed end opposite to the interface part, and a screen portion therebetween is inserted through a first port in a lid of a container, with the interface part providing access to an interior of the container for insertion of a transfer cannula connected to a syringe filled with the adipose tissue material. The screen portion includes a plurality of apertures having diameters of the selected size. Adipose tissue material is expelled from the transfer cannula through the apertures of the filter screen assembly into the container to create and collect the fat aspirate particles having particle diameters less than or equal to the selected size.

A deagglomerator for use in resizing masses of cells is disclosed. The deagglomerator may include a plurality of apertures defined by a plurality of front and back edges. The masses of cells may be passed through the plurality of apertures from the front to the back, and from the back to the front, repeatedly. The deagglomerator may also include a plurality of blades that may aid in the deagglomeration of the cell masses. The deagglomerator may be configured between two syringes so that the tissue may be passed back and forth from the first syringe through the device to the second syringe, and then back again from the second syringe through the device and to the first syringe. In this way, the masses of cells may be properly deagglomerated.

The present invention relates to a device and a method for FIG. 2 treating fat cells taken from a patient and intended for a transplant. More specifically, it relates to a device and a method by suctioning which makes it possible to separate the viable fat cells from the residues and elements which are not transplantable. The device is a device for treating adipose tissues (G) connected to a suctioning means (M), consisting of a sealed treatment chamber (A) comprising a means for connection (17) to said suctioning means (M), a means for inlet (13) of said adipose tissues before treatment, a filtration means (3) and at least one means for outlet (20) of said adipose tissues (G) after treatment, said filtration means (3) comprising a conically shaped external wall (3 1, 3) comprising a narrow base (55) attached to an impeller (25) and an internal sieving means (30) connected to the output means (20), said impeller (25) being subjected to the air pressure reduction created by the suctioning means (M) and constituting a rotary mechanical actuating means for actuating said filtration means (3).

A method of treating soft tissue conditions. A harvesting device is provided. The harvesting device is operably connected to a tissue processing device using tubing. An aperture is formed in a bone. The bone has an interior. The harvesting device is inserted through the aperture in the bone and into the interior of the bone. The harvesting device is manipulated to dissociate connective tissue progenitor cells in the interior of the bone. Tissue is aspirated from the interior of the bone. The connective tissue progenitor cells are separated from the aspirated tissue. The separated connective tissue progenitor cells are injected in a region of a body that is experiencing a soft tissue condition to treat the soft tissue condition.

A system and method are provided for minimally-invasive selective fat removal from a target area by injecting the area with a solution of photo-absorbing nanoparticles and irradiating the injected area with a beam of near infrared (NIR) light. The NIR emission wavelength excites the nanoparticles to melt fat within the target area so that the liquefied fat can be aspirated from the target area. The nanoparticles may be gold nanorods having aspect ratios selected to produce surface plasmon resonance when irradiated with NIR light around 800 nm.

Harvested adipose tissue is processed in a single-use portable processing container to prepare a centrifuges pellet phase of stromal vascular fraction concentrate, which is selectively removed and disposed in a syringe dispersed in a dispersion medium to prepare an osteoarthritis treatment product.

Disclosed are methods and devices for processing lipoaspirate that include mechanically-processing harvested lipoaspirate in a liposuction filter canister. In some embodiments, the devices are liposuction devices that include a lipoaspirate processing unit for mechanically-processing lipoaspirate. The mechanical processing reduces the average size of adipose tissue pieces in the lipoaspirate without substantially rupturing lipocytes therein.

A morselizer has a first end section with an axially and radially hollow luer fitting, a second end section with an axially and radially hollow luer fitting, a housing juxtaposed therebetween, a channel extending axially between the first end section and the second end section and through the housing providing an axial liquid pathway therein, and at least one blade disposed within the housing. Each end of the morselizer is attachable to a structure with a complimentarily sized luer fitting. In an embodiment, the morselizer facilitates atraumatic resizing of material through axial liquid transfer, by transferring material from a first structure attached to the first end section, thence through the channel, where material is resized by the blade, and through the second end section to a second structure attached to the second end section. The resized material may be suitable for injection, such as through a fine needle.

A portable apparatus useful for collection and processing of human biological material, such as adipose or cancellous bone material, to prepare a concentrated product (e.g., stromal vascular fraction). The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a pellet well for collecting concentrate product in the form of a pellet phase from centrifuge processing. The pellet well is accessible only from above when the apparatus is in an access orientation. Collected pellet phase material may be removed from the pellet well by direct aspiration, without suspending the material in a suspension liquid within the container. Access ports may be configured for access only from above the container. The apparatus may include a tissue collector disposed in the disuse retention volume to engage and collect collagen or other stringy tissue. A method of processing adipose tissue to concentrate leuko stromal vascular cells includes multi-step processing using a portable container.

Disclosed are methods and devices for processing lipoaspirate that include mechanically-processing harvested lipoaspirate in a liposuction filter canister. In some embodiments, the devices are liposuction devices that include a lipoaspirate processing unit for mechanically-processing lipoaspirate. The mechanical processing reduces the average size of adipose tissue pieces in the lipoaspirate without substantially rupturing lipocytes therein.