Systems and methods for electroporation are provided. An electroporation system includes a catheter including a plurality of electrodes, and a pulse generator coupled to the catheter, the pulse generator configured to generate a waveform to be delivered using at least one of the plurality of electrodes. The waveform includes a first pulse having a first polarity, a first pulse amplitude, and a first pulse width, and a second pulse having a second polarity, a second pulse amplitude, and a second pulse width, wherein the first and second pulses are separated by an interpulse delay, and wherein at least one of i) the first pulse amplitude is different than the second pulse amplitude and ii) the first pulse width is different than the second pulse width.

Provided are mechanisms and processes for enhancing absorption of therapeutic agents into the skin. Therapeutic agents may include lotions, creams, ointments, and solutions. Electrodes are used to apply a voltage to create an electric field to enhance penetration of the agent through the epidermal layer of the skin. The voltage applied may vary between 5V and 36V depending on the skin type or type of substance. The electrodes may be integrated into a wearable device such as a glove, sleeve, mask, bandage, etc.

Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.

The present invention provides for the intratumoral delivery of immunomodulators. In particular, it provides delivery of co-stimulatory molecules using intratumoral electroporation. The present invention provides a method for the treatment of malignancies, wherein the administration of a plasmid encoding for a therapeutic costimulatory protein, in combination with electroporation has a therapeutic effect on primary tumors as well as distant tumors and metastases.

Provided herein is a vaccine comprising a Porcine Epidemic Diarrhea Virus (PEDV) antigen. The antigen can be a consensus antigen. Also disclosed herein is a method of treating a porcine in need thereof, by administering the vaccine to the porcine.

A handling and control system for expandable electrodes of a handpiece is provided that includes a plurality of flexible electrodes made of elastic cables carried by a support assembly with needle-shaped front portions that protrude from the support assembly and move in a three-dimensional space under the push of actuators. An electronic control device performs the following functions: a) providing a command to the actuators to perform an initial handling of each cable according to an initial step Δh performing an axial advancement of the front portion with respect to the second proximal end and a distancing of the front portion from the axis H; b) determining for each pair of electrodes the spacing or distance l.sub.i, measured along a direction perpendicular to the axis, between the tips of the front portions of the pair of electrodes; c) determining a voltage V as a function of the spacing l.sub.i, V=f(l.sub.i) and applying to each electrode a pulsed signal having maximum voltage equal to the calculated value V; e) repeating the steps a), b) and c) for a plurality n of steps k successive to the initial one so that the active portions of the electrodes move in space in a three-dimensional application area becoming distanced from each other; the voltage applied to the electrodes increasing linearly with the increasing of the spacing so as to generate an electric field which ensures in the application area complete electro-poration of tissue.

A system for synchronizing application of treatment signals with a cardiac rhythm is provided. The system includes a memory that receives and stores a synchronization signal indicating that a predetermined phase such as R-wave of a cardiac rhythm of a patient has started. A synchronization module analyzes whether the stored synchronization signal is erroneous and if so, prevents a medical treatment device from applying a treatment energy signal such as an IRE pulse to a patient to take into account an irregular heart beat and noise in the synchronization signal in order to maximize safety of the patient.

Described is a device and method for administration of molecules to tissue in vivo for various medical applications, the device comprising a single hypodermic injection needle and at least two spaced elongate electrodes which provide for the ability, when the needle is inserted into tissue, such as skin or muscle, to pulse tissue with a non-uniform electric field sufficient to cause reversible poration of cells lying along or in close proximity to the track made by the needle upon its insertion into said tissue.

An adaptive control method for controlling EP pulse parameters during electroporation (EP) of cells or tissue using an EP system includes providing a system for adaptive control to optimize EP pulse parameters including EP pulse parameters, applying voltage and current excitation signals to the cells, obtaining data from the current and voltage measurements, and processing the data to separate the desirable data from the undesirable data, extracting relevant features from the desirable data, applying at least a portion of the relevant features to a trained diagnostic model, estimating EP pulsing parameters based on an outcome of the applied relevant features, where the initialized EP pulsing parameters are based on the trained model and the relevant features, to optimize the EP pulsing parameters, and applying, by the generator, a first EP pulse based on the first pulsing parameters.

An apparatus includes a body configured to be at least partially implanted on or within a recipient and a plurality of electrodes positioned along the body. The plurality of electrodes includes a first set of electrodes configured to apply electrical stimulation signals to at least a portion of the recipient. The plurality of electrodes further includes a second set of electrodes configured to apply an electric field to cell membranes of the recipient, the electric field configured to increase a permeability of the cell membranes to a substance.