A method for separating of at least two transition metals, the method comprising: injecting a feed solution into a chromatography column comprising a chromatographic support media, the feed solution comprising at least two transition metals; eluting the feed from the column in an elution cycle by flowing an eluent through the column, wherein a concentration of the eluent is reduced during the elution cycle prior to elution of at least one of the transition metals.

The technology generally relates to tailoring a back pressure regulator in a chromatographic system to reduce unswept volume within the back pressure regulator to achieve better sample detection and a reduction in chromatographic band distortion effects.

A system for blending solutions and a buffer solution is disclosed. In this system a switch valve is present capable of flowing one or more solutions, a low pressure pump for pumping the one or more solutions through the switch valve and a T-joint capable of receiving the one or more solutions through the low pressure pump and blending the one or more solutions with a buffer solution. A high pressure pump is present for collecting a blended solution.

A system for blending solutions and a buffer solution is disclosed. In this system a switch valve is present capable of flowing one or more solutions, a low pressure pump for pumping the one or more solutions through the switch valve and a T-joint capable of receiving the one or more solutions through the low pressure pump and blending the one or more solutions with a buffer solution. A high pressure pump is present for collecting a blended solution.

A methodology scales supercritical fluid chromatography (SFC) and/or carbon dioxide based chromatography methods between different system and/or column configurations. The methodology includes measuring an average mobile phase density during a first separation utilizing C02 as a mobile phase component and substantially duplicating the average density profile for a second separation. Substantial duplication of the average mobile phase density (e.g., within about 10%, 5%, 2.5%, 1%, 0.5%, 0.1 %, 0.05%) results in chromatography for both system and/or column configurations having similar selectivity and retention factors. Average mobile phase density may be, either measured directly, calculated, or approximated using average pressure or density measurements. The average pressure profile may be used as a close approximation to duplicate average density profiles between separations.

A methodology scales supercritical fluid chromatography (SFC) and/or carbon dioxide based chromatography methods between different system and/or column configurations. The methodology includes measuring an average mobile phase density during a first separation utilizing C02 as a mobile phase component and substantially duplicating the average density profile for a second separation. Substantial duplication of the average mobile phase density (e.g., within about 10%, 5%, 2.5%, 1%, 0.5%, 0.1 %, 0.05%) results in chromatography for both system and/or column configurations having similar selectivity and retention factors. Average mobile phase density may be, either measured directly, calculated, or approximated using average pressure or density measurements. The average pressure profile may be used as a close approximation to duplicate average density profiles between separations.

A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.

Methods for determining the relative abundance of viral capsid components in a sample of viral particles are disclosed. In embodiments, methods for determining the relative abundance of empty capsids, partially-full capsids and full capsids (e.g., containing a heterologous nucleic acid molecule) of adeno-associated virus are disclosed.

The present invention generally pertains to methods of characterizing charge variants of a protein of interest. In particular, the present invention pertains to the use of desalting size exclusion chromatography-reduced peptide mapping mass spectrometry to identify charge variants separated by capillary isoelectric focusing.

When a liquid in the column is replaced by carbon dioxide in a supercritical state in the chromatograph, an operation of a first pump is controlled by a flow rate control unit, and the carbon dioxide in the supercritical state is supplied at a constant pressure. Moreover, when a flow rate of the carbon dioxide in the supercritical state reaches a predetermined flow rate thereafter, the flow rate control unit controls an operation of the first pump so that the carbon dioxide in the supercritical state is supplied at a constant flow rate.