A separation matrix comprising porous particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is above 5 mg/ml, the volume-weighted median diameter of said porous particles is at least 10 and below 30 μm and the said porous particles have a gel phase distribution coefficient, expressed as K.sub.D for dextran of molecular weight 110 kDa, of 0.5-0.9.

A process and system for separating paraxylene from a mixture of paraxylene, metaxylene, orthoxylene, and ethylbenzene in a simulated moving bed apparatus using a membrane to separate non-aromatics from a desorbent stream. The lower nonaromatics content in the desorbent improves paraxylene product purity, increases paraxylene production at the same desorbent rate, reduces the desorbent rate, and/or reduces energy consumption in the product tower.

The present disclosure provides methods for producing consumable recombinant proteins that are substantially free from herein-disclosed undesired byproducts.

Compositions and methods for isolating L-glufosinate from a composition comprising L-glufosinate and glutamate are provided. The method comprises converting the glutamate to pyroglutamate followed by the isolation of L-glufosinate from the pyroglutamate and other components of the composition to obtain substantially purified L-glufosinate. The composition comprising L-glufosinate and glutamate is subjected to an elevated temperature for a sufficient time to allow for the conversion of glutamate to pyroglutamate, followed by the isolation of L-glufosinate from the pyroglutamate and other components of the composition to obtain substantially purified L-glufosinate. The glutamate alternatively may be converted to pyroglutamate by enzymatic conversion. The purified L-glufosinate is present in a final composition at a concentration of 90% or greater of the sum of L-glufosinate, glutamate, and pyroglutamate. In some embodiments, a portion of the glutamate in the starting composition may be separated from the L-glufosinate using a crystallization step. Solid forms of L-glufosinate materials, including crystalline L-glufosinate ammonium, are also described.

The disclosure relates to a method for separation and purification of N-acetyl-glucosamine, and belongs to the technical field of biological engineering. In the disclosure, a raw material solution containing N-acetyl-glucosamine is obtained by microbial fermentation or by hydrolyzing the chitin. The raw material solution is subjected to flocculation pretreatment, and continuous centrifugation or pressure filtration is performed to remove suspended solids such as microorganisms, proteins and polysaccharides to obtain clear liquid. Double-stage ion exchange chromatography is performed to remove impurities such as charged organic molecules and inorganic salts. Membrane concentration is performed to efficiently remove water to improve the concentration of the target product. Spray drying or further evaporation concentration and crystallization are performed. Finally drying is performed to obtain an N-acetyl-glucosamine crystal of which the purity is more than 99%.

The present invention relates to a method for separating one or more components from a liquid feed mixture in an EBA-SMB operating mode without the need of pumps at the outlets of the EBA columns. The present invention also relates to a simulated moving bed separation device with expanded bed adsorption columns which can be used in the method according to the invention.

The invention concerns a purification method comprising: providing a flow comprising a glycol, monovalent ions and multivalent ions; treating this flow with ion exclusion chromatography comprising: injecting the flow into a chromatographic unit comprising an ion exchange stationary phase; injecting an eluent into the chromatographic unit; collecting a fraction at the outlet of the chromatographic unit; the collected fraction being enriched with glycol and depleted of monovalent ions and multivalent ions relative to the flow.

The invention also concerns an installation adapted to implement this method, and its application to the regeneration of an anti-hydrate agent.

A process is described for making acrylic acid from dextrose, which comprises fermenting dextrose; removing solids from the resulting fermentation broth; removing lactic acid from the clarified broth by extraction into an organic solvent; separating out the lactic acid-loaded organic solvent while recycling at least a portion of the remainder back to the fermentation step; reacting the lactic acid with ammonia to provide a dehydration feed comprising ammonium lactate while preferably recycling the organic solvent; carrying out a vapor phase dehydration of the ammonium lactate to produce a crude acrylic acid product; and purifying the crude acrylic acid by distillation followed by melt crystallization, chromatography or both melt crystallization and chromatography.

A fractal flow device comprising at least one fractal pack. The at least one fractal pack comprises at least two fractal cells, where each fractal cell comprises a fractal distributor, a chamber adjacent the fractal distributor, and a fractal collector adjacent the chamber. Methods of using the fractal flow device are also disclosed.

A process and system for separating paraxylene from a mixture of paraxylene, metaxylene, orthoxylene, and ethylbenzene in a simulated moving bed apparatus using a membrane to separate non-aromatics from a desorbent stream. The lower nonaromatics content in the desorbent improves paraxylene product purity, increases paraxylene production at the same desorbent rate, reduces the desorbent rate, and/or reduces energy consumption in the product tower.