The disclosure relates to a method for separation and purification of N-acetyl-glucosamine, and belongs to the technical field of biological engineering. In the disclosure, a raw material solution containing N-acetyl-glucosamine is obtained by microbial fermentation or by hydrolyzing the chitin. The raw material solution is subjected to flocculation pretreatment, and continuous centrifugation or pressure filtration is performed to remove suspended solids such as microorganisms, proteins and polysaccharides to obtain clear liquid. Double-stage ion exchange chromatography is performed to remove impurities such as charged organic molecules and inorganic salts. Membrane concentration is performed to efficiently remove water to improve the concentration of the target product. Spray drying or further evaporation concentration and crystallization are performed. Finally drying is performed to obtain an N-acetyl-glucosamine crystal of which the purity is more than 99%.

The present disclosure relates to a method for preparing L-cysteine crystals, and L-cysteine crystals prepared by the method. Through the method for preparing L-cysteine crystals of the present disclosure, L-cysteine crystals can be obtained from a natural L-cysteine fermentation broth with a high recovery rate and/or purity without a chemical reaction or the use of an artificial synthetic compound.

The present disclosure provides chromatography columns and methods of chromatographic separation using the same. In particular, the disclosure provides modified columns where the cross-sectional area of the column increases in an area near the end of the column in a flared manner. The modified columns can be used in Expanded Bed Absorption (EBA) systems.

Embodiments of the hybrid thermal-chromatograph systems described herein solve the co-product generation problem associated with seawater desalination, and result in significant reduction in the selling price of fresh water generated through the process, while also solving problems associated with traditional lithium mining practices.

The present invention relates to a method for separating one or more components from a liquid feed mixture in an EBA-SMB operating mode without the need of pumps at the outlets of the EBA columns. The present invention also relates to a simulated moving bed separation device with expanded bed adsorption columns which can be used in the method according to the invention.

The present invention relates to a valve unit (100) for a chromatography apparatus, the valve unit comprising a fluid inlet (110) configured to receive an input fluid, a fluid outlet (120) configured to provide an output fluid, a first pair of fluid ports (131,132) configured to be coupled to a first column, a second pair (141,142) of fluid ports configured to be coupled to a second column, a coupling valve assembly (200) configured to direct fluid between a selection of the fluid inlet (110), the fluid outlet (120), the first pair of fluid ports (131,132) and the second pair of fluid ports (141,142) in response to one or more control signals, wherein the coupling valve assembly is configured to direct fluid using a selection of membrane valves coupled by fluid channels comprised in a body of the coupling valve assembly. The invention further relates to a chromatography apparatus comprising the valve unit and a membrane valve comprised in the valve unit.

Systems and methods use ion exchange to extract lithium from a lithium-containing feed solution such as a salar brine. Lithium ions are loaded into an ion exchange resin and then eluted while recharging the resin. Sodium hydroxide or sodium bicarbonate may be used to recharge the resin but are not directly mixed with the lithium-containing feed solution. An eluate stream is produced containing lithium hydroxide or lithium bicarbonate. Lithium hydroxide can be precipitated as lithium hydroxide or in a hydrate form. Lithium bicarbonate may be converted to lithium carbonate. The system and method optionally includes processing an eluate stream to recover one or more compounds for re-use in regenerating the resin bed.

Compositions and methods for isolating L-glufosinate from a composition comprising L-glufosinate and glutamate are provided. The method comprises converting the glutamate to pyroglutamate followed by the isolation of L-glufosinate from the pyroglutamate and other components of the composition to obtain substantially purified L-glufosinate. The composition comprising L-glufosinate and glutamate is subjected to an elevated temperature for a sufficient time to allow for the conversion of glutamate to pyroglutamate, followed by the isolation of L-glufosinate from the pyroglutamate and other components of the composition to obtain substantially purified L-glufosinate. The glutamate alternatively may be converted to pyroglutamate by enzymatic conversion. The purified L-glufosinate is present in a final composition at a concentration of 90% or greater of the sum of L-glufosinate, glutamate, and pyroglutamate. In some embodiments, a portion of the glutamate in the starting composition may be separated from the L-glufosinate using a crystallization step. Solid forms of L-glufosinate materials, including crystalline L-glufosinate ammonium, are also described.

The present invention relates to a method of preparing kestose-containing fructooligosaccharide, and more specifically, a method of preparing kestose-containing fructooligosaccharide having a high content of kestose and excellent storage stability.

The present disclosure relates to an improved method for producing allulose and, more particularly, to a method for preparing a fructose-containing raw material solution by using raw sugar as a raw substrate used in the production process.