The present disclosure relates to a method for preparing L-cysteine crystals, and L-cysteine crystals prepared by the method. Through the method for preparing L-cysteine crystals of the present disclosure, L-cysteine crystals can be obtained from a natural L-cysteine fermentation broth with a high recovery rate and/or purity without a chemical reaction or the use of an artificial synthetic compound.

A purification system for separating a molecule of interest from a solution is provided and generally includes a plurality of resin processing sections, wherein each of the resin processing sections is controllably separated from each other via a configurable valve, wherein the configurable valve is configured to introduce or evacuate a fluid/resin into/out of a proximately located processing section, a plurality of inlets configured to introduce fluid and/or resin into one or more of the plurality of resin processing sections.

A high salinity wastewater treatment system is provided according to the present application, which includes a hydrogel loading system and a flow-storage different-oriented-inlet-and-outlet system. The hydrogel loading system includes six separation plates, a wastewater treatment area, a water distribution bin, a rotating shaft, a driving motor and a fixed bracket. The six separation plates evenly separate the wastewater treatment area into six separate treatment sectors in an axial direction. The six separate treatment sectors are filled with hydrogel materials with water purification effect. The high salinity wastewater infiltrates into each separate treatment sector one by one through high salinity wastewater inlet meshes on a surface of the wastewater treatment area, and the purified high salinity wastewater is discharged through a wastewater cleaning outlet pipe with a same water inlet direction as a cleaning filler distribution pipe.

A method for producing full spectrum hemp extract comprising extracting substances from cannabis-based green material that are soluble in an extraction solvent and collecting an extract that includes the extraction solvent distilling at least a portion of the extraction solvent which results in a concentrate that is not distilled off, removing at least a portion of water soluble substances from the concentrate by partitioning the at least a portion of water soluble substances into an aqueous phase and a remainder of substances from the concentrate into a partitioned concentrate, heating the partitioned concentrate to evaporate the nonpolar solvent and to yield a crude oil, degassing the crude oil by heating it which results in a degassed crude oil, performing a first pass distillation at about 150° C. and collecting a first residue, performing a second and third pass distillation at about 170° C. and about 185° C., and collecting a distillate from same.

A protein purification process with virus inactivation or removal uses caprylic acid (octanoic acid) at acidic pH. The method comprises caprylic acid treatment as part of the chromatographic step so as to perform viral inactivation without a discontinuous process and without the requirement of attention by personnel, particularly when the pH adjustment of the eluate is performed automatically by eluting into buffer. The method of the invention further results in mycoplasmas being inactivated, and reduced impurities like Host Cell Protein (HCP).

The invention provides a plurality of methods which may be employed as a single method or a plurality of sequential method steps in the isolation and purification of Cannabidiol from Hemp plants. It also provides methods for having a terpene-rich fraction as a byproduct of the method. Depending upon the ultimate objective, the individual method components may be used separately or combined with one or more method steps to accomplish the objectives.

The present invention relates to a method for simultaneously separating cannabidivarin and cannabigerol, comprising: sufficiently oscillating a solvent system, allowing the solvent system to stand, and separately collecting an upper phase and a lower phase; dissolving a commercially available industrial hemp full-spectrum refined oil into the upper phase, performing separation using high-speed countercurrent chromatography with the upper phase as a stationary phase and the lower phase as a mobile phase to obtain a mixture of cannabidivarin and the mobile phase and a mixture of cannabigerol and the mobile phase, respectively, and removing the mobile phase to obtain cannabidivarin and cannabigerol. The present invention uses high-speed countercurrent chromatography to simultaneously separate and purify to obtain cannabidivarin (CBDV) with the purity of greater than 98% and cannabigerol (CBG) with the purity of greater than 97% from the industrial hemp full-spectrum refined oil for the first time.

Provided herein are, inter alia, biological manufacturing and downstream purification processes.

Assemblies, systems, and methods for isolation of target material are provided. In various embodiments, an assembly for target material isolation includes a housing having an upper portion and a lower portion together defining an inner chamber. The inner chamber includes a semi-toroidal shape and the semi-toroidal shape defines a longitudinal axis. The assembly further includes one or more fluidic connection from the exterior of the housing to the inner chamber. An isolation material (e.g., polymer wool and/or magnetic beads) may be disposed within the inner chamber. A system includes a configured to fit at least a portion of the housing and releasably couple the assembly. Upon activation of the motor, the assembly may rotate about the longitudinal axis. An angle of the platform may be adjustable to thereby change the angle of the longitudinal axis about which the assembly rotates.

This document describes a process for the high purity and high concentration recovery of monovalent products via continuous ion exchange from aqueous solution for further down-stream purification.