A solid fiber is described, where the solid fiber is characterized by (a) a modification degree Do/Di, in a cross section of the solid fiber of 1.20 to 8.50 where the inscribed circle diameter is denoted by Di and the circumscribed circle diameter is denoted by Do; and (b) a porous specific surface area of not less than 30 m.sup.2/g.

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a superficially porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, chemical stability, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.

The present invention provides a separation material that comprises porous polymer particles comprising a styrene-based monomer as a monomer unit; and a coating layer comprising a macromolecule having hydroxyl groups, which covers at least a portion of the surface of the porous polymer particles, and the separation material has a 5% compressive deformation modulus of 100 to 1,000 MPa, and has a mode diameter in the pore size distribution of 0.1 to 0.5 μm.

The present application relates to the technical field of further processing of dairy products, and in particular to a preparation method of milk oligosaccharides, and milk oligosaccharide powder and food prepared thereby. The preparation method comprises the steps of: performing ultrafiltration of whey liquid for at least three times, subjecting the ultrafiltration permeate to nanofiltration concentration for several times, then subjecting the nanofiltration retentate to chromatographic separation and purification, collecting chromatographic collection liquid containing sialyllactose while removing the fraction containing lactose, subjecting the collection to desalination and drying to obtain oligosaccharide powder. The milk oligosaccharides prepared by the present method and the food product containing the same comprise basically bovine milk oligosaccharides, which are light yellow or white in color, light in flavor, uniform in size, and have good thermal stability and solubility. The milk oligosaccharides mainly comprise 3′-sialyllactose and 6′-sialyllactose.

The invention concerns a method for producing a chromatography analysis column, the resulting column, and a device comprising such a column. The method according to the invention comprises the following steps: (a) depositing on the flat surface of a substrate a first layer of particles which are intended to form the stationary phase; (b) depositing on the layer at least one second layer of compactly assembled particles; (c) impregnating the first and second layers with a light radiation-sensitive material, to form at least two compactly assembled particle layers impregnated with sensitive material; (d) insolating these layers in the regions corresponding to the desired internal shape of the chromatography analysis column, if the light radiation-sensitive material behaves like a positive resin, or outlining this internal shape if the light radiation-sensitive material behaves like a negative photosensitive resin; (e) eliminating either the regions insolated in step (d) if the light radiation-sensitive layer behaves like a positive photosensitive resin, or the regions not insolated in step (d) if the light radiation-sensitive material behaves like a negative photosensitive resin; and (f) covering and sealing the structure obtained in step (e) with a cover covered on the face facing the layers with at least one layer of compactly assembled particles which are identical to or different from those deposited on the substrate surface. The invention is used in particular in the field of chemical analysis.

An apparatus for suppressing an eluent of an aqueous sample stream including analyte ions of one charge, positive or negative, comprises a primary channel member, a first block, a first regenerant flow channel, a first charged barrier, a second block, a second regenerant flow channel, a second charged barrier, a first stationary flow-through ion exchange material, and optionally a first electrode and a second electrode. The first stationary flow-through ion exchange material comprises a polyolefin substrate having a functional polymer layer disposed thereon. The polyolefin substrate has a pore structure with a pore size ranging from about 5 microns to about 250 microns. The functional polymer layer has a thickness ranging from about 1 micron to about 20 microns, and a layer pore structure having a pore size ranging from about 1 nm to about 100 nm. The functional polymer layer comprises an ion exchange layer.

The disclosure provides methods for the isolation, separation, and purification of antibodies. The method comprises an affinity chromatography capture step, anion exchange chromatography polishing step, and cation exchange chromatography polishing step.

A solid phase for use in separation has been modified using an aqueous phase adsorption of a headgroup-modified lipid to generate analyte specific surfaces for use as a stationary phase in separations such as high performance liquid chromatography (HPLC) or solid phase extraction (SPE). The aliphatic moiety of the lipid adsorbs strongly to a hydrophobic solid surface, with the hydrophilic and active headgroups orienting themselves toward the more polar mobile phase, thus allowing for interactions with the desired solutes. The surface modification approach is generally applicable to a diversity of selective immobilization applications such as protein immobilization clinical diagnostics and preparative scale HPLC as demonstrated on capillary-channeled fibers, though the general methodology could be implemented on any hydrophobic solid support material.

The present invention relates to a chromatography column (100; 100′) comprising a tubular side wall (110) having an inner wall (112), an adaptor assembly (120) and a base assembly (125). An enclosed bed space (130) is defined between said adaptor assembly (120), said base assembly (125) and said inner wall (112) of said tubular side wall (110). The adaptor assembly (120) is axially movable inside said tubular side wall (110) in relation to said base assembly (125). The inner wall (112) comprises at least one groove (180; 180′; 180″; 180′″) which is positioned in a lower half of the tubular side wall (110) of the chromatography column (100), via which groove (180; 180′; 180″; 180′″) air and other fluid can pass the adaptor assembly (120) when the adaptor assembly is positioned in a priming position, in which priming position said adaptor assembly intersects with at least a part of the at least one groove (180; 180′; 180″; 180′″).

There are provided porous fibers having excellent removal performance with respect to a material to be purified; and a purification column into which an adsorbent material obtained by bundling the fibers is incorporated. The porous fibers satisfying the following conditions (a) and (b) and having a shape in which three or more projected parts are continuously present in the lengthwise direction on the periphery part of a solid-state fiber: (a) The modification degree Do/Di in a cross section is 1.2 to 6.6 when the diameter of the inscribed circle is denoted by Di and the diameter of the circumscribed circle is denoted by Do., and (b) The specific surface area of pores is 50 m.sup.2/g or more.