The present invention is in the field of purification and protein purification in particular. The invention provides improved techniques for the industrial-scale purification of proteins and other biomolecules. More specifically, it relates to a process for the purification of a compound of interest, such as a protein, preferably an antibody or an antibody fragment using a chromatography step, preferably a semi-continuous chromatography step.

The present disclosure relates to methods of modifying complex extracts such that components or mixtures of components are selectively removed or added, thus providing a complex mixture that does not naturally occur with a refined or a tuned therapeutic or nutraceutical effect. In various aspects, the complex extract can be an extract obtained from one or more plants, e.g., an extract obtained from green tea leaves. The present disclosure pertains to compositions obtained by the disclosed methods, nutraceutical compositions comprising same, pharmaceutical compositions comprising same, and methods of treating various conditions, including physiological dysfunctions associated with elevated reactive oxygen species and/or inflammatory molecule, e.g., TNFα, expression using same. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

A sample separation network includes a server node, a plurality of client nodes coupled with the server node, a plurality of sample separation devices coupled with the server node, wherein each of the sample separation devices includes device-specific control software configured for controlling specifically the respectively assigned sample separation device, wherein at least one of the server node and the client nodes includes generic control software configured for generically controlling sample separation devices in a non-device-specific way, and wherein at least one of the server node and the client nodes and the sample separation devices is configured for loading device-specific control software from a sample separation device to at least one of the server node and the client nodes upon connection of said sample separation device to the sample separation network.

A separation column (6), an injector (4) that injects a sample into a mobile phase flowing through a flow path (16) leading to the separation column (6), at least one detector (8;10) that is fluidly connected downstream of the separation column (6), and generates a plurality of detector signals different from each other derived from a component in a sample separated by the separation column (6), a fraction collector (12) for fractionating and collecting a portion containing a component separated by the separation column (6) in an eluate from the separation column (6) into an individual collection container, and a controller (14) that detects a component peak in a plurality of chromatograms based on each of a plurality of the detector signals and controls operation of the fraction collector (12) based on a result of the detection are included. The controller (14) includes a fractionating collection part (26) configured to execute fractionating collection in which a portion detected as a component peak only in one chromatogram of a plurality of the chromatograms and a portion detected as a component peak in all of a plurality of the chromatograms are collected separately in collection containers different from each other.

The invention concerns a purification method comprising: providing a flow comprising a glycol, monovalent ions and multivalent ions; treating this flow with ion exclusion chromatography comprising: injecting the flow into a chromatographic unit comprising an ion exchange stationary phase; injecting an eluent into the chromatographic unit; collecting a fraction at the outlet of the chromatographic unit; the collected fraction being enriched with glycol and depleted of monovalent ions and multivalent ions relative to the flow.

The invention also concerns an installation adapted to implement this method, and its application to the regeneration of an anti-hydrate agent.

The present invention relates to a preparative chromatography system (200, 500, 800) and a chromatography process (400, 700) adapted to repetitive cycling of chromatography volumes. The system (200, 500, 800) comprises at least two upstream pumps (203a, 803a, 203b, 803b) and separate flow paths (220) from process liquid sources to the chromatography device (200, 500, 800). The system (200, 500, 800) is arranged to prime one flow path (220) with one process liquid while providing another process liquid to the chromatography device and thereby minimizing the hold-up volume of the system (200, 500, 800).

A supercritical fluid chromatography system comprises a first pump for pumping a first flow stream comprising a compressible fluid and a second pump for pumping a second flow stream comprising a modifier fluid. The second pump is in parallel with the first pump. A column is located in a combined flow stream. The column is located downstream of the first and second pumps. The combined flow stream comprises the first flow stream, the second flow stream, and a sample. A detector is located downstream of the column. A gas-liquid separator is located downstream of the detector. The gas-liquid separator is configured to vent a majority of the compressible fluid while maintaining a majority of the sample, thus preventing aerosolization of the flow stream and minimizing sample loss as well as cross contamination. An open bed collector is located after the gas-liquid separator.

A target component is collected using a preparative separation and purification device having a holder for holding a trap column in which the target component has been captured, a liquid feeder for feeding a first solvent having compatibility with the water remaining in the trap column and a second solvent having low compatibility with water and high compatibility with the first solvent into the trap column, a flow-path switch for connecting the exit end of the trap column to a waste liquid flow path and a collection flow path, and a control unit for controlling the flow-path switch so that solution including water flows into the waste liquid flow path.

A lamp-housing assembly, for a detector of a sample separation apparatus for separating a fluidic sample, includes a lamp seat, a lamp insertable into the lamp seat, and a lamp cap mountable on the lamp seat and on the inserted lamp. The lamp seat, lamp and lamp cap are matched with respect to each other so that, by inserting the lamp into the lamp seat and by mounting the lamp cap on the lamp seat and on the inserted lamp, the lamp is axially and radially aligned and electrically and thermally coupled with the lamp seat and the lamp cap.

A preparative chromatograph system includes a sample injector (10) that injects a sample into a separation flow path (8) through which a mobile phase flows, a separation column (12) that is provided on the separation flow path (8) and is to separate a sample injected into the separation flow path (8) into components, a detector (14) that is provided at a position farther downstream than the separation column (12) on the separation flow path (8) and is to detect sample components obtained by separation in the separation column (12), a collector (18, 18′) having a plurality of collection containers (22) for collecting part of an eluate eluted from the separation column (12) and a switch mechanism (20, 30, 32) configured to be capable of selectively connecting a downstream end of the separation flow path (8) to any one of the collection containers (22), a collection setter (26) configured to set a portion, to be collected in each of the collection containers (22), of an eluate eluted from the separation column (12) using a pre-acquired chromatogram of a sample, and a collection setter (26) configured to be capable of setting a portion, including a sample component, of an eluate eluted from the separation column (12), and a portion, between two sample components that are adjacent to each other on the chromatogram, of an eluate eluted from the separation column (12), as the portion to be collected, and a controller (28) configured to control an operation of the switch mechanism (20, 30, 32) of the collector (18, 18′) and collect the portion, which is to be collected and is set by the connection setter (26), in the predetermined collection container (22).