The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

This present invention is directed to variable affinity chromatography apparatus and methods for using the same. In particular, the polarity of the stationary phase or the mobile phase is modulated using an external stimulus. Exemplary external stimulus that can be used in the invention include, but are not limited to, electric field, electromagnetic radiation including UV, Vis, and infrared wavelengths, as well other stimuli that are known to one skilled in the art. Generally, any external stimulation that changes the polarity of a stimulus responsive material can be used. One particular embodiment of the invention provides a chromatography apparatus comprising: (i) a chromatography column having a stationary-phase separation medium contained therein; (ii) an external stimulus generator operatively connected to said chromatography column; and (iii) a chromatography mobile-phase, wherein at least one of said stationary-phase separation medium and said chromatography mobile-phase comprises a stimulus responsive material that adopts a different configuration based on the absence or the presence of said external stimulus, wherein different configurations of said stimulus responsive material results in a different stationary or mobile phase affinity, and wherein said external stimulus is selected from the group consisting of electric field, electromagnetic radiation, and a combination thereof.

The invention relates to membranes for separation, removal, and/or concentration purposes. The object of the invention is the simple and reliable adsorption of the molecules and to simplify the desorption of target molecules that are adsorbed and chromatographically bonded on membranes, preferably without the addition of substances with a high ion content, such as acids, alkalis or salts. The object of the invention is also to develop a value that can be easily measured, which allows for an indication of the current and/or remaining binding capacity of the membrane during the adsorption process and/or the control thereof. The adsorption takes place on a charged membrane and desorption is achieved using physical, electromagnetic and/or the generation of electrical fields. This is carried out with a thin metal layer being applied to one or both sides of a positively or negatively charged membrane and a voltage is applied for desorption.

A manufacturing method of an operation pipe, which use a gel to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components that are biological components such as nucleic acids. More specifically, a manufacturing method of an operation pipe, with which it is possible to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components in a sealable pipe by operating magnetic particles in the pipe under a magnetic field from outside of the pipe.

There is provided a method of extracting material from a fluid method of extracting material from a fluid, the fluid being held within a fluid chamber. The method comprises drawing, with a magnetic field generating system, at least one magnetically susceptible member through the fluid around a closed path between at least three points in the chamber, said at least one member being adapted to bind to material in fluid in the chamber. The at least three points are arranged relative to each other in a shape having at least two dimensions, the magnetic field generating system being configured to move the at least on magnetically susceptible member directly between the at least three points, material in the fluid binding to the at least one magnetically susceptible member when it comes into contact with the at least one member as it moves through the fluid.

A chromatograph is provided for identifying components of a mixture. Components are identified by different rates of adsorption and/or desorption with a material phase. In one embodiment, an electrical lead is connected to the material phase for supplying an electrical charge to the material phase. The electrical charge alters the rate of adsorption/desorption of the components with the material phase. In another embodiment, the material phase is disposed between two conductors with electrical leads connected to each of the conductors. A charge differential between the two conductors alters the rate of adsorption and/or desorption of components with the material phase.

Exemplary embodiments of the present invention can include a method for isolating a contaminate from water comprising: introducing a plurality of aluminum-doped nanoparticles to water, the water comprising the contaminate; contacting the plurality of aluminum-doped nanoparticles with the contaminate to form contaminate-adsorbed nanoparticles; and isolating the contaminate-adsorbed nanoparticles by applying a magnetic field to the water.

Embodiments of the present disclosure can include a method for isolating a contaminate from water comprising: introducing a plurality of aluminum-doped nanoparticles to water, the water comprising the contaminate; contacting the plurality of aluminum-doped nanoparticles with the contaminate to form contaminate-adsorbed nanoparticles; and isolating the contaminate-adsorbed nanoparticles by applying a magnetic field to the water.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

Disclosed is a microvesicles isolation method to isolate microvesicles contained in the biological sample from the sample, the method comprising: (a) adding an adsorbent sphere to the biological sample containing the microvesicles therein; (b) keeping the adsorbent sphere in the biological sample to form an adsorbent sphere conjugate composed of the adsorbent sphere and the microvesicles captured thereon; (c) isolating the adsorbent sphere conjugate from the biological sample; (d) washing the isolated adsorbent sphere conjugate using a first reagent; and (e) eluting the microvesicles from the washed adsorbent sphere conjugate using a second reagent, wherein the adsorbent sphere includes a support, and one or more polyvalent cations disposed on a surface of the support.