A method of improving volumetric productivity from a cell culture includes filtering a cell culture through an ultrafilter or a microfilter operating in tangential flow filtration mode or alternating flow filtration mode, and filtering the cell culture concurrently or intermittently through a tangential flow depth filtration system to remove cellular debris and/or to harvest cell product. A system for filtering biological materials includes a primary filtration system, and a secondary filtration system, where the secondary filtration system comprises a tangential flow depth filtration filter.

The invention generally relates to the filed of providing a liquid for human consumption. In particular, the invention relates to a system for channeling a liquid such as particularly an aqueous liquid such as water in a circuit and for controlling the contamination of the circulating liquid with microorganisms, as well as to a corresponding method using the same. Furthermore, the invention relates to a method for the effective energy saving in the course of providing a heated liquid for human consumption while controlling the limit values recommended, admissible or acceptable for microorganisms, in which the set temperature of a heating device (8) is adjusted to a value below 60° C., preferably to a value between 40 and 55° C., most preferably to a value between 43 and 48° C.

A liquid infused membrane includes a porous fluorine-containing polymer membrane and a perfluoropolyether oil coating on at least a portion of the first surface and at least a portion of the pore wall. Advantageously, the liquid infused membrane does not exhibit gating. Methods for the manufacture thereof and uses of the liquid infused membrane are also disclosed.

A water desalination system includes a first set of ultrafiltration membranes, a second set of ultrafiltration membranes, a first backwashing system configured to treat at least one of the first set of ultrafiltration membranes or the second set of ultrafiltration membranes with brine generated by a reverse osmosis process, and a second backwashing system configured to treat at least one of the first set of ultrafiltration membranes or the second set of ultrafiltration membranes with one or more chemicals and reverse osmosis permeate water.

The present disclosure provides a method for treating the surface of a microporous polymeric membrane, comprising immersing a microporous polymeric membrane in a liquid; and applying ultrasonic waves to the microporous polymeric membrane immersed in the liquid.

Methods and devices are disclosed for a separation device. A separation device includes a separation module having a separation membrane separating an interior of the separation module into a retentate compartment and a permeate compartment. The retentate compartment includes at least one retentate channel, a feed port fluidly coupled to the at least one retentate channel and a retentate port. The permeate compartment includes at least one permeate channel disposed within the permeate compartment and a permeate port fluidly coupled to the at least one permeate channel, a retentate collector fluidly connected to the retentate port. The device further includes a feed reservoir, a permeate reservoir, a fluidic gate located between the feed reservoir and the separation module, a vent located between the retentate channel and the permeate channel end adjacent the adjacent the retentate port and a pressure differential source applied across the separation module.

A method for reducing a contaminating DNA content of a preparation containing AAV capsids and contaminating DNA, comprising the steps of a) Performing an extraction of DNA with a solid phase bearing positive charges at its surface said solid phase is contacted with the preparation at a pH of 7.0±1.0, and a salt concentration of 10 mM to 200 mM yielding a first fraction, (b) Diafiltering the first fraction by a first tangential flow filtration to obtain a second fraction, (c) Treating the second fraction with DNase, (d) Diafiltering the DNase treated second fraction obtained by step c) by a second tangential flow, (e) filtration to a buffer with pH of 7.0±1.0, and a salt concentration of 10 mM to 20 mM to yield a third fraction, and optionally (f) Concentrating the third fraction by tangential flow filtration before supplemental chromatography.

The water treatment apparatus of the present invention comprises a flocculation part into which water to be treated is introduced, at least two flocculant adding devices installed so that different flocculants can be added to the flocculation part, one or two or more water quality measurement devices for measuring the quality of the water to be treated, and a controlling part for issuing, on the basis of the measurement result from the water quality measurement device(s), a command relating to whether addition of the flocculants to the corresponding flocculant adding device is required or not and to the added amounts of the flocculants, wherein at least one of the flocculant adding devices is an auxiliary flocculant adding device.

Disclosed are plant protein compositions prepared from hemp plant material. Hemp seeds are deconstructed and separated into heavy protein-rich fractions and light oil-rich fractions. The deconstruction processes avoid the use of organic solvents and temperatures above 60° C. The separated fractions are then selectively processed to produce selected plant protein containing compositions comprising from about 30% (w/w) to about 95% hemp protein, from about 5% (w/w) and to about 60% (w/w) plant oil. The plant protein compositions from hemp plants are suitable for use as ingredients in nutritional formulations.

In accordance with the invention, provided herein are methods for purifying recombinant adeno-associated (rAAV) vector particles.