A device for generating bubbles, comprising a porous material having at least one hydrophilic surface (1), arranged such that a liquid (7) in which the bubbles (6) are intended to be formed may contact the hydrophilic surface (1) and at least one hydrophobic surface (2), arranged such that a gas (5) used to generate the bubbles (6) may flow past the hydrophobic surface (2) before it flows past the hydrophilic surface (1). The device may be used for creating fine bubbles in numerous applications, such as wastewater treatment, plant cultivation, aquafarming, aeration systems, bioreactors, fermeters, oil extraction or fuel cells.

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

A magnetic mixing apparatus is disclosed. A magnetic mixing apparatus according to one embodiment of the present invention comprises: a shaft which passes through a driving motor unit and which is rotatably coupled to the driving motor unit; a first housing which is coupled to one side of the driving motor unit and which is provided outside a stirring container to accommodate an upper part of the shaft; a rotary rotor which is coupled to the end of the shaft at a stirring container side during normal operation and which has a first permanent magnet; and an impeller unit which is arranged inside the stirring container and which has a second permanent magnet magnetically coupled to the first permanent magnet during normal operation, wherein the first housing includes a magnetic separation rotary rotor support part formed to protrude inward so as to support, from the downward direction, the rotary rotor, which is down-driven so as to move downward from the shaft, when there is magnetic separation by which magnetic coupling is released through magnetic deformation of the first permanent magnet and/or the second permanent magnet.

A device (30) includes a base connector (32) having an opening (33) and an impeller connector (64) coupled to the base connector (32). The impeller connector (64) has a through-passage (66) aligned with the opening (33) of the base connector (32). Further, the device (30) includes a flexible tube (34) having a first end (36) and a second end (40), where the first end (36) of the flexible tube (34) is coupled to the impeller connector (64). Furthermore, the device (30) includes a seal component (38) and an impeller (42) coupled to the second end (40) of the flexible tube (34). Additionally, the device (34) includes an enclosure (46) disposed enclosing the impeller (42), the flexible tube (34), the impeller connector (64), and the base connector (32).

An impeller, for example, a Rushton impeller for a bioreactor system is disclosed. The impeller includes a hub, optionaly including a slot, a plurality of blades, and one or more turbulators. The plurality of blades is disposed along a circumferential direction of the hub and spaced apart from each other. Each of the plurality of blades is coupled to at least a portion of a circumference and/or a top surface of the hub. Each blade of the plurality of blades includes a pressure face and a suction face. The one or more turbulators is disposed on at least a portion of the suction face, the pressure face, or both, of a blade of the plurality of blades.

A reactor system includes a reactor vessel configured to contain a process fluid, and a sparger assembly that operably coupled to the reactor vessel and configured to supply a mixture of a gas and a recirculated process fluid to the reactor vessel. The sparger assembly includes a plurality of sparger chambers. Each sparger chamber includes a process fluid conduit fluidly coupled to a process fluid return of the reactor vessel via a process fluid inlet, wherein the process fluid inlet has a first block and bleed valve assembly. Each sparger chamber includes a sparger conduit fluidly coupled to the process fluid conduit and a sparger disposed within the sparger conduit and fluidly coupled to a gas source via a gas inlet. Each sparger chamber also includes a process fluid-gas mixture outlet that fluidly couples the sparger conduit to a sparger outlet of the reactor vessel.

The present set of embodiments relate to a system, method, and apparatus controlling a cell culture media mixing system. The control system includes an integrated control unit capable of controlling a broad ranges of peripheral devices commonly used in bioproduction through a graphical user interface displayed on a touch sensitive screen. The bioproduction system is designed to be highly customizable through process modification and recipe creation by user with little or no knowledge of programming and is capable of controlling a wide variety of devices using a single unit. The bioproduction system allows for auto-detection, auto-calibration, and automatic of device related processes into a bioproduction workflow.

The present invention relates to a bioreactor assembly comprising a plurality of trays (30) for holding a cell culture bag, a holder (2) on which the trays are mounted side by side to form a first series of trays rocking mechanism (4) for rocking the trays, the rocking mechanism (4) being operatively connected to each of the trays (30). The invention also relates to a bioreactor system (100) comprising at least one bioreactor assembly.

A reactor system includes a support housing having an interior surface bounding a chamber, the chamber having a vertically extending central longitudinal axis. A flexible bag is disposed within the chamber of the support housing and has an interior surface bounding a compartment. A mixing element is disposed within the compartment of the flexible bag. A drive element, such as a drive shaft, is secured the mixing element, wherein the mixing element is laterally offset from and/or is angled relative to the vertically extending central longitudinal axis of the support housing.

A stirring device of a plug-flow fermentation device includes a shaft rotatable about an axis of rotation which defines an axial direction. The stirring device further includes a boundary stirring element that defines the axial extent of a stirring volume covered by the stirring device. A nearest neighbor of the boundary stirring element in the axial direction has an axial maximum width which is smaller than an axial maximum width of the boundary stirring element and which is larger than an axial maximum width of a next-nearest neighbor of the boundary stirring element.