A method for preparing fluorescent-encoded microspheres coated with metal nanoshells is disclosed herein. By using SPG method, metal nano-material modified with a certain ligand is used as a new surfactant in the emulsification process, and different kinds and different amounts of fluorescent materials are doped into polymer microspheres to prepare fluorescent-encoded microspheres with different fluorescent-encoded signals and uniformly coated metal nanoshells in one step. The prepared fluorescent-encoded microsphere comprises a metal nanoshell, a polymer, and a fluorescent-encoded material. The fluorescent-encoded microsphere has a particle size of 1 μm˜20 μm, CV of less than 10%, which can be used for protein/nucleic acid detection. The preparation method has the advantages of simple process, high surface coating rate, good uniformity and controllable LSPR peaks, which can solve the problems of existing commonly used metal nanoshell coating methods such as low surface coating rate, poor uniformity, complex preparation process and uncontrollable local surface plasmon resonance (LSPR) peaks, etc.

Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.

Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.

A method for preparing fluorescent-encoded microspheres coated with metal nanoshells is disclosed herein. By using SPG method, metal nano-material modified with a certain ligand is used as a new surfactant in the emulsification process, and different kinds and different amounts of fluorescent materials are doped into polymer microspheres to prepare fluorescent-encoded microspheres with different fluorescent-encoded signals and uniformly coated metal nanoshells in one step. The prepared fluorescent-encoded microsphere comprises a metal nanoshell, a polymer, and a fluorescent-encoded material. The fluorescent-encoded microsphere has a particle size of 1 μm˜20 μm, CV of less than 10%, which can be used for protein/nucleic acid detection. The preparation method has the advantages of simple process, high surface coating rate, good uniformity and controllable LSPR peaks, which can solve the problems of existing commonly used metal nanoshell coating methods such as low surface coating rate, poor uniformity, complex preparation process and uncontrollable local surface plasmon resonance (LSPR) peaks, etc.

Concentrate filling system includes a solid loader operable to dispense particles through a dispensing end. The system includes a conveyer operable to receive the dispensed particles at a proximal end and further dispense particles at a distal end. The system includes load cell operable to receive a container that receives the dispensed particles. The system includes an injection pump to dispense fluids into the container. The system includes controller operable to receive data from the load cell indicative of a weight of the particles and/or fluid and send a control signal to the conveyor to adjust the dispensing of the particles and send a control signal to the injection pump. Upon receiving indicative of a predetermined weight of the container, fluid, and particles, the controller is operable to send a control signal to the conveyor and the injection pump to stop dispensing of particles and fluid.

Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.

Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.

Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.

Concentrate filling system includes a solid loader operable to dispense particles through a dispensing end. The system includes a conveyer operable to receive the dispensed particles at a proximal end and further dispense particles at a distal end. The system includes load cell operable to receive a container that receives the dispensed particles. The system includes an injection pump to dispense fluids into the container. The system includes controller operable to receive data from the load cell indicative of a weight of the particles and/or fluid and send a control signal to the conveyor to adjust the dispensing of the particles and send a control signal to the injection pump. Upon receiving indicative of a predetermined weight of the container, fluid, and particles, the controller is operable to send a control signal to the conveyor and the injection pump to stop dispensing of particles and fluid.