A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

An impeller, for example, a Rushton impeller for a bioreactor system is disclosed. The impeller includes a hub, optionaly including a slot, a plurality of blades, and one or more turbulators. The plurality of blades is disposed along a circumferential direction of the hub and spaced apart from each other. Each of the plurality of blades is coupled to at least a portion of a circumference and/or a top surface of the hub. Each blade of the plurality of blades includes a pressure face and a suction face. The one or more turbulators is disposed on at least a portion of the suction face, the pressure face, or both, of a blade of the plurality of blades.

A tool for dry mixing and coating of powder mixtures comprising includes a fixing shaft and an approximately disc-shaped element fixed thereto and of a diameter d, having an upper surface, a lower surface and a peripheral surface connecting the upper and lower surfaces. The disc-shaped element has a plurality of grooves extending parallel to the shaft axis. Each groove has two groove walls extending from the peripheral surface to a groove bottom. A tooth is formed between two grooves. In order to provide a tool which is suitable for particularly low contamination dry mixing, dry dispersing and coating of powder mixtures, the disc-shaped element has a base portion and at least one wear element fixed to the base portion, wherein at least one portion of each groove wall, that adjoins the peripheral surface, is formed by the wear element.

A tool for dry mixing and coating of powder mixtures comprising includes a fixing shaft and an approximately disc-shaped element fixed thereto and of a diameter d, having an upper surface, a lower surface and a peripheral surface connecting the upper and lower surfaces. The disc-shaped element has a plurality of grooves extending parallel to the shaft axis. Each groove has two groove walls extending from the peripheral surface to a groove bottom. A tooth is formed between two grooves. In order to provide a tool which is suitable for particularly low contamination dry mixing, dry dispersing and coating of powder mixtures, the disc-shaped element has a base portion and at least one wear element fixed to the base portion, wherein at least one portion of each groove wall, that adjoins the peripheral surface, is formed by the wear element.

The present disclosure provides advantageous rotary interfaces for fluid assemblies (e.g., rotary interfaces for fluid flow in bioreactor applications), and related methods of fabrication and use. More particularly, the present disclosure provides improved rotary interfaces for fluid flow through porous impellers for filtration and/or sparging for fluid assemblies (e.g., bioreactor applications), and related methods of fabrication and use. Disclosed herein is a fluid assembly (e.g., bioreactor) that includes a porous impeller which is in fluid communication with a hollow shaft that can be used to transport a reaction fluid to an external storage tank or the like. The fluid assembly/bioreactor can include a coupling mechanism that transmits rotary motion from a motor to a primary shaft and then to a hollow secondary shaft, while at the same time permitting removal of a filtrate from the fluid assembly or bioreactor via the hollow secondary shaft and a porous impeller.

The present disclosure provides advantageous rotary interfaces for fluid assemblies (e.g., rotary interfaces for fluid flow in bioreactor applications), and related methods of fabrication and use. More particularly, the present disclosure provides improved rotary interfaces for fluid flow through porous impellers for filtration and/or sparging for fluid assemblies (e.g., bioreactor applications), and related methods of fabrication and use. Disclosed herein is a fluid assembly (e.g., bioreactor) that includes a porous impeller which is in fluid communication with a hollow shaft that can be used to transport a reaction fluid to an external storage tank or the like. The fluid assembly/bioreactor can include a coupling mechanism that transmits rotary motion from a motor to a primary shaft and then to a hollow secondary shaft, while at the same time permitting removal of a filtrate from the fluid assembly or bioreactor via the hollow secondary shaft and a porous impeller.

A horizontal mixer for mixing bulk material, such as agricultural material, is provided. The horizontal mixer includes a mixing container for accommodating bulk agricultural material to be mixed. The mixing container includes side walls connected at their bottom edges by a trough; a front wall spanning a front side of the container; a rear wall spanning a rear side of the container; and an exit door through which mixed agricultural material can exit. Further, one or more bottom augers situated longitudinally in the trough, wherein at least one of the one or more bottom augers is a ribbon auger; and one or more top augers situated longitudinally in the mixing container stacked above the one or more bottom ribbon augers. The trough has a shape to accommodate the one or more bottom ribbon augers.

A machine for providing a single serving of a cold beverage or a frozen confection is disclosed. The machine includes a cooling system, a first assembly, and a second assembly. The first assembly includes the cooling system and has sidewalls partially defining a recess for receiving a pod containing at least one ingredient for forming the single serving of the cold beverage or frozen confection and at least one internal paddle. The second assembly has a lid and a plunger movably mounted to the lid. The plunger is operable to engage a top of the pod and rotate the at least one internal paddle of the pod to scrape the cold beverage or a frozen confection off a wall of the pod and mix contents of the pod.

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.