A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

An apparatus for nitrogenizing a beverage utilizing a container having a chamber with a plurality of baffles. The container also includes a nitrogen regulator in the form of a collapsible orifice at the base of the container. An agitator in the form of an impeller creates a shear on the beverage during delivery of a stream of nitrogen into the container.

There is provided a stirring device including a stirring tank including an inner peripheral wall which is circular in cross section, at least one circulating impeller and at least one dispersion blade which are located inside the stirring tank and rotatable around a vertical axis independently of each other, and a guide ring disposed radially outward near the dispersion blade. The circulating impeller is disposed along the inner peripheral wall of the stirring tank, and rotates around the vertical axis to form at least a downward flow in a stirring object existing inside the stirring tank. The dispersion blade rotates to apply a shear force to the stirring object, and is disposed at a radially inner position of the stirring tank from the circulating impeller, and at a position in contact with a flow of the stirring object, which is formed by the circulating impeller.

There is provided a stirring device including a stirring tank including an inner peripheral wall which is circular in cross section, at least one circulating impeller and at least one dispersion blade which are located inside the stirring tank and rotatable around a vertical axis independently of each other, and a guide ring disposed radially outward near the dispersion blade. The circulating impeller is disposed along the inner peripheral wall of the stirring tank, and rotates around the vertical axis to form at least a downward flow in a stirring object existing inside the stirring tank. The dispersion blade rotates to apply a shear force to the stirring object, and is disposed at a radially inner position of the stirring tank from the circulating impeller, and at a position in contact with a flow of the stirring object, which is formed by the circulating impeller.

An expanded particle producing apparatus includes a vessel and a blade stirrer that is located inside the vessel. The blade stirrer comprises a stirrer base and an impeller blade that is attached to the stirrer base. A distance from the bottom of the vessel to the center of the stirrer base (L1) and a depth of the vessel (L2) have a ratio (L1/L2) of 0.01 to 0.2. L1 is measured in a depth direction parallel to L2, and a central axis of the blade stirrer coincides with a central axis of the vessel. The apparatus is configured to produce expanded particles.

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

A single stage clarifier and mixing assembly includes a housing, a mixing section within the housing and a clarifier section within the housing. The mixing section includes a mixing chamber having (a) an inlet, adapted for delivering an inlet stream to the mixing chamber, at an upper end, and (b) a mixing section outlet at a lower end. The clarifier section extends concentrically around the mixing section. The single stage clarifier and mixing assembly also includes an agitator adapted for mixing the inlet stream in the mixing chamber.

A single stage clarifier and mixing assembly includes a housing, a mixing section within the housing and a clarifier section within the housing. The mixing section includes a mixing chamber having (a) an inlet, adapted for delivering an inlet stream to the mixing chamber, at an upper end, and (b) a mixing section outlet at a lower end. The clarifier section extends concentrically around the mixing section. The single stage clarifier and mixing assembly also includes an agitator adapted for mixing the inlet stream in the mixing chamber.

A blender system includes a container having a rotatable blade assembly therein, a lid covering an open end of the container, and a base with a motorized unit. When the container is coupled with the base, the motorized unit is adapted to drive rotation of the rotatable blade assembly. The lid includes a hinged actuator lever adapted to actuate the motorized unit. A detent extends from the hinged actuator lever and passes through a series of apertures and presses against a slidable actuator shaft disposed in the container and maintained in its position by a spring force, thereby depressing a switch for the motorized unit. A rotatable blade system includes cutting blade(s) and crushing blade(s). The crushing blade extends longitudinally outwardly from a hub. When the hub rotates in a first direction or second direction opposite the first direction, a face of the crushing blade is leading or trailing, respectively.