A device for separating one or more molecules of interest in a liquid specimen including a monolithic body defining a fractionation column. The column includes an inlet opening at a proximal end of the fractionation column; an outlet opening at a distal, opposite end of the fractionation column; a solid phase chamber positioned between the inlet opening and the outlet opening; a specimen introduction area adjacent a proximal end of the solid phase chamber; an analyte exit area adjacent a distal end of the solid phase chamber; an inlet chamber adjacent the inlet opening that tapers into the specimen introduction area; and an outlet chamber that extends from the analyte exit area to the outlet opening. A metered amount of solid phase packed within the solid phase chamber between a first porous frit and a second porous frit of the solid phase chamber.

A method for analysing a crude oil to determine the amount of organic acid compounds contained in the crude oil includes extracting the organic acid compounds from a sample of crude oil to form an extract and determining the amount of the extracted organic acids In addition, the method includes dissolving the extract in a polar solvent to form a solution of the extracted organic acid compounds Further, the method includes introducing a sample of the solution of the extracted organic acid to an apparatus including a reversed phase liquid chromatography (LC) column and a mass spectrometer (MS) arranged in series. The reversed phase LC column contains a hydrophobic sorbent and the mobile phase for the LC column includes a polar organic solvent. Still further, the method includes separating the organic acid compounds in the LC column of the LC-MS apparatus and continuously passing the separated organic acid compounds from the LC column to the MS of the LC-MS apparatus to ionize the organic acid compounds and to obtain a chromatogram with mass spectral data over time for the ionized organic acid compounds. Moreover, the method includes determining the area(s) under the peak(s) in an extracted ion chromatogram derived from the mass spectral data assigned to one or more organic acid compounds. The method also includes determining the amount of the organic acid compound(s) in the sample by comparing the area under the peak(s) assigned to the organic acid compound(s) with the area under a peak in an extracted ion chromatogram assigned to a specific amount of a standard organic acid compound. In addition, the method includes extrapolating from the amount of the organic acid compound(s) in the sample to provide the total amount of the organic acid compound(s) in the extract.

Provided is a separation method for amine, the separation method including performing liquid chromatography, wherein a separating agent in which a ligand having a crown ether-like cyclic structure is supported on a carrier is used as a stationary phase, and wherein a mobile phase contains an aqueous solution of at least one salt of a hydrophobic anion selected from the group consisting of a salt of a chaotropic anion and a salt of a hydrophobic organic acid.

A chiral stationary phase comprises a porous framework material and biomolecules. The porous framework material includes one of the metal-organic framework (MOF) material, the covalent organic framework (COF) material and the hydrogen-bonded organic framework (HOF) material. The biomolecules are biological chiral resolving agents. A pore size of the porous framework material is 0.2-15 nm. The porous framework material serves as a solid carrier. The biomolecules are loaded into the porous framework material. The porous framework material is modified with one or more of carboxyl, hydroxyl, amino, aldehyde, double bonds and mercapto groups.

A chiral stationary phase comprises a porous framework material and biomolecules. The porous framework material includes one of the metal-organic framework (MOF) material, the covalent organic framework (COF) material and the hydrogen-bonded organic framework (HOF) material. The biomolecules are biological chiral resolving agents. A pore size of the porous framework material is 0.2-15 nm. The porous framework material serves as a solid carrier. The biomolecules are loaded into the porous framework material. The porous framework material is modified with one or more of carboxyl, hydroxyl, amino, aldehyde, double bonds and mercapto groups.

The present disclosure generally relates to methods for separating Δ.sup.8-THC, Δ.sup.9-THC, and related enantiomers using CO.sub.2-based chromatography.

The present disclosure generally relates to methods for separating Δ.sup.8-THC, Δ.sup.9-THC, and related enantiomers using CO.sub.2-based chromatography.

Provided is a separating agent for optical isomers, which is excellent in solvent resistance and has optical separating ability comparable to or higher than that of existing separating agents for optical isomers of chemical bonding type or physical adsorption type. In the separating agent for optical isomers, amylose (3-chloro-5-methylphenylcarbamate) is supported on a carrier through chemical bonding.

Provided is a separating agent for optical isomers, which is excellent in solvent resistance and has optical separating ability comparable to or higher than that of existing separating agents for optical isomers of chemical bonding type or physical adsorption type. In the separating agent for optical isomers, amylose (3-chloro-5-methylphenylcarbamate) is supported on a carrier through chemical bonding.

A method for separating diastereomers of pristane. A pristane sample is prepared, and then injected into a chromatographic instrument equipped with a chiral chromatographic column, where a stationary phase of the chiral chromatographic column has a preset pore size. The pristane diastereomers in the pristane sample are separated by the chiral chromatographic column, and the components produced by the separation of the pristane diastereomers sequentially enter a mass spectrometer for detection and analysis.