Chromatography resins having mixed mode ligands and methods of using such resins are provided.

The present invention relates to a multimodal adsorption medium, in particular a multimodal chromatography medium, a method for its production, as well as use of the adsorption medium according to the invention or an adsorption medium produced according to the invention for the purification of biomolecules.

Provided herein are stationary phase compositions comprising a chromatographic surface of porous or non-porous core material comprising a surface modifier for use in chromatographic separations.

The present disclosure provides a preparation method of AM-type polystyrene microsphere ofloxacin imprinted polymer as well as an application thereof. A monomer acrylamide and an initiator ammonium persulfate are subjected to graft polymerization on the surface of modified polystyrene primary amine resin, to get grafted particles; then an adsorption test of a levofloxacin solution by the grafted particles PAM/PSA is conducted, and then a levofloxacin surface molecularly imprinted material MIP-PAM/PSA is prepared by using ethylene glycol diglycidyl ether as the crosslinking agent. The present disclosure can realize the separation and purification of racemic ofloxacin effectively, thus providing a new method and material for separating and enriching s-type ofloxacin in the industry. Because the antibacterial efficacy of S-ofloxacin on Gram-negative bacteria and positive bacteria is 8-128 times that of its enantiomer R-ofloxacin, so the present technology can improve the efficacy of a drug greatly.

Chromatography resins having mixed mode ligands and methods of using such resins are provided.

Functionalised polymeric chromatography medium, comprising: at least one non-woven sheet comprising one or more polymeric nanofibers having a mean diameter of 10-1000 nm; one or more polymer chains grafted onto the one or more polymeric nanofibers, wherein the polymer chains are poly-glycerol chains comprising glycidol monomer residues or wherein the polymer chains comprise divinylsulfone monomer residues; and at least one ligand group bonded to the one or more polymer chains.

Disclosed herein is macroporous controlled porosity silica gel (CPSG) covalently modified with a variety of moieties (e.g., moieties suitable for oligonucleotide synthesis comprising a spacer, a linker and a nucleoside, chromophore, ligand or bioconjugation linker, or a spacer and a universal linker). Also disclosed herein are methods of making the covalently-modified, macroporous CPSG, and methods of using the covalently-modified, macroporous CPSG to synthesize oligonucleotides.

The present invention provides a functionalised polymeric chromatography medium, prepared by a process which comprises (i) providing a substrate formed of one or more polymer nanofibres, (ii) grafting one or more neutral polymer chains from the substrate, and (iii) contacting the grafted product with a reagent which functionalises the product of step (ii) as a chromatography medium, wherein step (ii) comprises reacting a plurality of compounds of formula and/or its enantiomers, and/or its derivatives of formula (I) and/or enantiomers and/or diastereomers thereof: with one or more functional groups present on the nanofibre substrate, wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 may be the same or different, and are chosen from H, halogen, C.sub.1-C.sub.4 alkyl, or C.sub.1-C.sub.4 alkoxy provided that at least one of R.sub.1, R.sub.2, R.sub.3, R.sub.4 or R.sub.5 is not hydrogen. ##STR00001##

In various aspects, the present disclosure pertains to high purity chromatographic materials that comprise a chromatographic surface wherein the chromatographic surface comprises a hydrophobic modifier and an ionizable modifier comprising one or more anion exchange moieties that are positively charged when ionized, as well as devices containing such materials. In other aspects, the present disclosure provides methods for mixed mode, anion exchange reversed phase liquid chromatography comprising: (a) loading a sample comprising a plurality of acidic analytes (e.g., acidic glycans) onto a chromatographic separation device comprising such a high purity chromatographic material and (b) eluting adsorbed acidic analytes from the high purity chromatographic material with a mobile phase comprising water, organic solvent, and an organic acid salt, wherein during the course of elution a pH of the mobile phase, an ionic strength of the mobile phase, and a concentration of the organic solvent are altered over time.

Provided herein are stationary phase compositions comprising a chromatographic surface of porous or non-porous core material comprising a surface modifier for use in chromatographic separations.