Methods of passively sampling PFAS in the environment, PFAS sorbents, apparatus and systems (apparatus plus conditions) for sampling groundwater, porewater, and surface water are described.

Described are composite materials and methods of using them for mixed-mode chromatography. In certain embodiments, the composite material comprises a support member, comprising a plurality of pores extending through the support member; and a multi-functional cross-linked gel. The multi-functional cross-linked gel possesses at least two of the following functions or characteristics: cationic, anionic, hydrophobic, hydrophilic, thiophilic, hydrogen bond donating, hydrogen bond accepting, pi-pi bond donating, pi-pi bond accepting, or metal chelating. The composite materials may be used in the separation or purification of a biological molecule or biological ion.

Disclosed is a device for a solid phase extraction comprising two or more of the sorbents to remove phospholipids and salts from a sample, to thereby eliminate matrix effects during mass spectrometry analysis. In particular, the sorbents includes at least one sorbent which is water-wettable and contains at least one hydrophobic component and at least one hydrophilic component and at least one of sorbent having a specific affinity for a matrix interference like phospholipids. Further disclosed is a method using the device of the present invention.

A porous polymer monolith comprises a polymer body having macroporous through-pores that facilitate fluid flow through the body and an array of mesopores adapted to bind from the fluid flow molecules of a predetermined range of sizes, wherein the surface area of the monolith is predominantly provided by the mesopores. Also disclosed is a method of making a porous polymer monolith. The method includes forming a polymer body by phase separation out of a solution containing at least a monomer, a crosslinker and a primary porogen, whereby the body contains multiple macroporous through-pores, wherein the solution further contains a secondary porogen comprising oligomers inert with respect to the monomer and cross-linker but chemically compatible with the monomer so as to form mesostructures within the polymer body during said phase separation, and washing the mesostructures from the body to provide an array of mesopores such that the surface area of the monolith is predominantly provided by the mesopores.

Contaminate-sequestering coatings including a network of hydrolyzed silane compounds including a plurality of thiol functional groups, a plurality of fluorinated functionalities, or both are provided. The contaminate-sequestering coatings may sequester one or more per- and polyfluoroalkyl substances (PFAS), heavy metals, biological species or any combination thereof. Methods of functionalizing a substrate surface with contaminate-sequestering functionalities that sequester one or more PFAS, heavy metals, or both are also provided. Methods of removing contaminants from contaminate-containing liquids, and devices including the contaminate-sequestering coatings are also provided.

A mixed mode affinity chromatography carrier includes a substrate, a hydrophilic polymer, an antibody-binding cyclic peptide, and a cation exchange group.

Contaminant-sequestering coatings include a network of hydrolyzed silane compounds including (i) a plurality of fluorinated functionalities, and (ii) a plurality of thiol functional groups are provided. The network of hydrolyzed silane compounds includes a fluorinated silane including (a) a hydrophilic polar head region. The polar head region includes one or multiple units of ethylene glycol (EG) functionality, (b) a fluorine-containing region, and (c) an anchor region including a silicon atom. The contaminant-sequestering coatings may sequester one or more per- and polyfluoroalkyl substances (PFAS), heavy metals, biological species, or any combination thereof.

Stationary phase for preparative High Pressure Liquid Chromatography (HPLC) for preparative separation of Rare Earth Elements (REEs), the stationary phase comprising porous particles suitable for HPLC having a non-polar surface being impregnated with ligands binding REEs, wherein the porous particles has a pore size of 300 Å or higher, is described.

The present invention relates to a method for the separation and purification of glycoforms with an ion exchange separation material with amino-acid based endgroups.

This present invention is directed to variable affinity chromatography apparatus and methods for using the same. In particular, the polarity of the stationary phase or the mobile phase is modulated using an external stimulus. Exemplary external stimulus that can be used in the invention include, but are not limited to, electric field, electromagnetic radiation including UV, Vis, and infrared wavelengths, as well other stimuli that are known to one skilled in the art. Generally, any external stimulation that changes the polarity of a stimulus responsive material can be used. One particular embodiment of the invention provides a chromatography apparatus comprising: (i) a chromatography column having a stationary-phase separation medium contained therein; (ii) an external stimulus generator operatively connected to said chromatography column; and (iii) a chromatography mobile-phase, wherein at least one of said stationary-phase separation medium and said chromatography mobile-phase comprises a stimulus responsive material that adopts a different configuration based on the absence or the presence of said external stimulus, wherein different configurations of said stimulus responsive material results in a different stationary or mobile phase affinity, and wherein said external stimulus is selected from the group consisting of electric field, electromagnetic radiation, and a combination thereof.