A chromatographic material including a substrate having a surface and having a polymeric layer covalently bound to the surface; the polymeric layer comprising polymer molecules covalently attached to the surface of the substrate, each polymer molecule being attached to the surface via multiple siloxane bonds and each polymer molecule being connected to one or more functionalizing compounds that each comprise a functional group, wherein the polymeric layer is formed by covalently attaching polymer molecules to the surface of the substrate via multiple siloxane bonds, each polymer molecule containing multiple first reactive groups, and reacting the first reactive groups of the attached polymer molecules with at least one functionalizing compound that comprises a second reactive group that is reactive with the first reactive groups and that further comprises a functional group. Preferred conditions of reacting the polymer with the substrate include elevated temperature and reduced pressure.

This invention relates to a new stationary phase carrying functional groups comprising a halogen substituted aromatic ring. Target molecules can interact with this stationary phase by halogen bonding. The stationary phase is suitable for SPE or chromatographic separations.

Functionalized particulate support material and chromatographic media prepared therefrom are disclosed. The functionalized particulate support material is a plurality of particles, each particle having a particle surface. Chemically bonded to and extending from the surface of the particles is a combination of hydrophobic and hydrophilic functional groups. The hydrophobic functional groups enable polymerization of one or more monomers onto the particle surface while the hydrophilic functional groups provide increased wettability of the particle surface compared to an unmodified particle surface. The functionalized particulate support material may be further processed so as to form polymer chains extending from the hydrophobic functional groups. In one embodiment, the resulting polymer functionalized material is useful as a chromatographic media in chromatography columns or cartridges, such as in a liquid chromatography (HPLC) column. Chromatography columns or cartridges containing the polymer functionalized media, and methods of making and using the media, are also disclosed.

A method reduces variation of measured data when nucleic acid is separated from a very small amount of sample followed by detection of the nucleic acid, wherein the reduction of the variation is achieved by removing contaminant nucleic acid in a nucleic acid purification column. The method of separating the nucleic acid from the sample containing the nucleic acid includes bringing the sample containing the target nucleic acid into contact with a nucleic acid-binding solid-phase carrier capable of adsorbing the nucleic acid; and eluting the nucleic acid from the nucleic acid-binding solid-phase carrier to which the nucleic acid is adsorbed.

The present disclosure features a composition, including molecularly imprinted crosslinked polymers that have been imprinted with trimethylamine N-oxide. The molecularly imprinted crosslinked polymers have specific binding sites for trimethylamine N-oxide, and a trimethylamine N-oxide absorption capacity of at least 0.5 mg/g.

An object of the present invention is to provide a purification column which is a small purification column in which a volume of a liquid to be treated is reduced in order to reduce the amount of blood to be taken out and which realizes a low pressure loss and has high adsorption performance. The present invention provides a fiber bundle including a plurality of porous fibers that satisfies the following requirements (A) to (E):

(A) the porous fiber has a non-hollow shape,

(B) an arithmetic average roughness (dry Ra value) of a surface of the porous fiber in a dry state is 11 nm or more and 30 nm or less,

(C) an arithmetic average roughness (wet Ra value) of a surface of the porous fiber in a wet state is 12 nm or more and 40 nm or less,

(D) a value represented by wet Ra/dry Ra is 1.05 or more, and

(E) a linear rate of the fiber bundle represented by (length of fiber bundle)/(length of one porous fiber) is 0.97 or more and 1.00 or less.

The subject invention provides materials, devices and methods for detecting, determining, monitoring and/or extracting trace metals such as cadmium, lead, copper, chromium, cobalt, nickel, zinc, manganese, mercury, and vanadium in the environmental, biological, pharmaceutical, and potable water samples. The subject invention also provides formulations and method for synthesizing the trace metal-extracting materials.

A polymer for liquid chromatography or solid phase extraction is provided. The polymer is prepared by polymerizing styrene and divinylbenzene to form a styrene-divinylbenzene copolymer; soaking the styrene-divinylbenzene copolymer in a swelling agent to form nano-scale micropores; and soaking the microporous styrene-divinylbenzene copolymer in methanol. When packed in a chromatographic column, the polymer can be used to produce produce natural health or medicinal products from Cannabis species, for example, industrial hemp.

The present invention discloses analytical high throughput methods for accurately, reliably, and efficiently screening and identifying polymers that are substantive to a particular material, such as hydroxyapatite. The present invention also discloses liquid chromatography columns for screening and identifying polymers that are substantive to a particular material, methods of preparing such liquid chromatography columns, and kits that may be used to screen and identify polymers that are substantive to a particular material.

A solid fiber is described, where the solid fiber is characterized by (a) a modification degree Do/Di, in a cross section of the solid fiber of 1.20 to 8.50 where the inscribed circle diameter is denoted by Di and the circumscribed circle diameter is denoted by Do; and (b) a porous specific surface area of not less than 30 m.sup.2/g.