A device for processing a sample comprises a blister defined by first and second walls. The first wall is flexible allowing the blister to be divided into one or more sealed regions by an external pressure applied to a portion of the first wall. The external pressure is applied in the form of a 2-dimensional shape to form a sealed region having that shape.

A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem including an actuation substrate, and a set of pins interacting with the actuation substrate, and a spring plate configured to bias at least one pin in a configurations, the valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; and a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols.

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR.

Laboratory on chip and its layered manufacturing method, wherein the method includes: designing, by means of a computer program, a printed circuit (7), mixing and reaction cavities (3) of fluids, microchannels (2) and spaces (15) for the placement of electronic components to be found in each layer, mechanizing in one or more biocompatible substrates the different voids and passages that will make up the mixing and reaction cavities (3), microchannels (2), holes (8) that join the microchannels and spaces for the subsequent placement of electronic components (15), metallizing with a biocompatible conductive material those surfaces in which the printed circuit will be integrated (7) according to the design performed in the first step, generating the printed circuit (7) by photolithography and acid attack, bonding the electronic components in the corresponding spaces (15), joining all the layers that make up the final laboratory.

A microfluidic chip is disclosed herein. In a specific embodiment, the microfluidic chip comprises at least one microfluidic reservoir having a wall portion and a heat transfer sealing layer cooperating with the wall portion for receiving a sample to be tested. The heat transfer sealing layer is arranged to be contiguous with the sample to be tested. The microfluidic chip further comprises an active temperature control device arranged to provide structural support to the heat transfer sealing layer and operable to control a temperature of the sample via transmission of heat through the heat transfer sealing layer. A detection module is also disclosed.

A device for performing in situ genetic analyses, conformed so as to be transportable manually by a user, which comprises a casing defining an internal compartment and a plurality of analysis units arranged in the internal compartment, where each analysis unit is configured to perform a respective and independent genetic analysis of at least one sample; each analysis unit comprises a sample holder compartment accessible by the user and adapted to accommodate at least one sample; a command and control unit; at least one sensor selected among an optical, acceleration, temperature, pressure, motion, chemical sensor or a combination thereof, configured to detect a first physical quantity relative to the genetic analysis of the at least one sample and to transduce the first physical quantity into a first signal which is indicative of the state of progress of the genetic analysis, the command and control unit is in signal communication with the at least one sensor for receiving said first signal; the analysis unit further comprises a plurality of instruments, configured to perform the genetic analysis of the sample, comprising an amplification and optical detection device configured to detect at least a second physical quantity relative to the genetic analysis and to transduce the second physical quantity into at least a second signal which is indicative of the outcome of the genetic analysis, the command and control unit is in signal communication with the amplification and optical detection device for receiving said second signal; the device further comprises a processing unit, in signal communication with the command and control unit of each analysis unit for receiving the respective first signals and the respective second signals.

The present disclosure relates to a structure capable of simultaneously purifying and detecting a nucleic acid by directly applying a sample, and more particularly, to a structure capable of performing sample preparation, loop-mediated isothermal amplification, detection and analysis steps on a single chip by applying lab-on-paper technology, and capable of finally determining whether the disease or bacterial is infected by moving the sample in a lateral flow method.

The present invention provides a test device for nucleic acid, including a sample treatment chamber, a sample reaction chamber and a test chamber which are disposed from top to bottom successively. The functions of sampling, nucleic acid purification, isothermal amplification and testing result reading by immunochromatography are integrated onto a device to prepare a simple and cute test device for nucleic acid; the device can achieve nucleic acid testing only by two rotations and can ensure that each sampling or reagent adding can reach the target area in a free falling way without any extra drainage facility. The present invention has lower costs, more convenient operation, high detection sensitivity, short time required in nucleic acid testing, and can be used for nucleic acid testing of various samples, such as human and animals.

A single device for testing each of total cholesterol, HDL, and triglyceride concentrations of a whole blood sample is disclosed. The device includes an inlet (10) for blood plasma and a transfer element (200) in fluid communication with the inlet (10), the transfer element (200) including a plurality of channels (210, 220, 230), each channel allowing capillary flow of blood plasma from the inlet (10) to a respective testing region (1, 2, 3). A channel (230) has a multiplicity of corners (235) which define a zigzag profile.

A tunable inertial sheathing (TIS) system and methods for particle-size-insensitive high-throughput single-stream focusing of particles suspended in a particle-carrying fluid are provided. The TIS conditions particles to distribute locally within one of compartments of inertial force field, followed by an inertial focusing to migrate it to a single foci. For the particle localization, the TIS system introduces an arbitrary form of peripheral sheathing by generating and accumulating sheath fluid from particle-carrying fluid through a combination of inertial focusing, channel bifurcation and channel confluence. Multiple forms of the TIS system are also provided, each including one main channel and at least one bypass channel. The main channel includes and cascades at least three segments, at least one bifurcating junction and at least one confluence junction.