The present disclosure relates to a method of manufacturing a microfluidic device, which may precisely form a channel having a desired shape within one substrate using a wax regardless of a shape of a hydrophilic porous substrate, and more specifically, to a method of manufacturing a microfluidic device in which a microchannel is formed by a wax within one hydrophilic porous substrate, the method including: an operation of stacking and then heat-treating a transfer film on which a mirror image of a wax pattern is formed to form a microchannel and the substrate.

Disclosed herein is a system to detect and characterize individual particles and cells using at least either optic or electric detection as the particle or cell flows through a microfluidic channel. The system also provides for sorting particles and cells or isolating individual particles and cells.

The present inventive concept relates to a microfluidic arrangement (1) for capillary driven fluidic connection between capillary flow channels (8, 16). The microfluidic arrangement (1) comprises: a first microfluidic system (4) comprising a first surface (5), and a first capillary flow channel (8), wherein the first capillary flow channel (8) has an elongation in a first plane, and the first surface comprises an outlet opening (9) in a plane different from the first plane, the outlet opening defining an outlet area (35) in the first surface and being adapted to allow fluidic communication with the first capillary flow channel thereby forming a flow outlet (12) of the first capillary flow channel, and a second microfluidic system (6) comprising a second surface (7) and a second capillary flow channel (16), wherein the second capillary flow channel (16) has an elongation in a second plane parallel to the first plane, and a portion of the second surface (7) comprises an inlet opening (13) in a plane different from the second plane, the inlet opening defining an inlet area (33) in the second surface and being adapted to allow fluidic communication with the second capillary flow channel thereby forming a flow inlet (20) of the second capillary flow channel, wherein the first microfluidic system (4) and the second microfluidic system (6) are arranged with the first and the second surfaces in contact such that the flow outlet (12) and the flow inlet (20) are interfaced, thereby allowing capillary driven fluidic connection between the first and the second capillary flow channels (8, 16), wherein the outlet area (35) overlaps at least a portion of the inlet area (33), said at least a portion of the inlet area (33) overlapped by the outlet area (35) being smaller than the outlet area (35).

Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.

The present disclosure provides apparatuses, systems, and methods involving a spotter apparatus for depositing a substance from a carrier fluid onto a deposition surface in an ordered array, the spotter apparatus comprising a loading surface including a first well and a second well; and a different outlet surface, including a first opening and a second opening, where a first microconduit fluidly couples the first well with the first opening and a second microconduit fluidly couples the second well with the second opening. An overlay is sealed to the outlet surface and penetrated by a deposition channel that is situated to communicate carrier fluid among the first opening, the second opening, and the deposition surface when the overlay is pressed against the deposition surface.

Laboratory on chip and its layered manufacturing method, wherein the method includes: designing, by means of a computer program, a printed circuit (7), mixing and reaction cavities (3) of fluids, microchannels (2) and spaces (15) for the placement of electronic components to be found in each layer, mechanizing in one or more biocompatible substrates the different voids and passages that will make up the mixing and reaction cavities (3), microchannels (2), holes (8) that join the microchannels and spaces for the subsequent placement of electronic components (15), metallizing with a biocompatible conductive material those surfaces in which the printed circuit will be integrated (7) according to the design performed in the first step, generating the printed circuit (7) by photolithography and acid attack, bonding the electronic components in the corresponding spaces (15), joining all the layers that make up the final laboratory.

A microporous substrate for detection of surface bound target analyte molecules includes a microporous substrate material having opposed surfaces and tapered micropores extending through the substrate with the micropores having wider openings on one side of the substrate compared to the other side. The micropores have bound therein analyte specific receptors complementary to the target molecules. When a liquid sample containing the target analyte molecules with optical probes attached to the target molecules is flowed through the substrate, they bind to their complementary analyte specific receptors and emit light. This microporous substrate structure gives an increase in the collection efficiency of light emitted from optical probes when the light is detected by a light detector spaced from the side of the microporous substrate facing the larger micropores openings compared to a light collection efficiency of light emitted from the optical probes when the micropores are straight and not tapered.

A microfluidic chip configuration wherein injection occurs in an upwards vertical direction, and fluid vessels are located below the chip in order to minimize particle settling before and at the analysis portion of the chip's channels. The input and fluid flow up through the bottom of the chip, in one aspect using a manifold, which avoids orthogonal re-orientation of fluid dynamics. The contents of the vial are located below the chip and pumped upwards and vertically directly into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. Then the channel takes a short horizontal turn that nearly negates any influence of cell settling due to gravity and zero flow velocity at the walls. The fluid is pumped up to a horizontal analysis portion that is the highest channel/fluidic point in the chip and thus close to the top of the chip, which results in clearer imaging. A laser may also suspend cells or particles in this channel during analysis which prevents them from settling.

A method of manufacturing a microplate including a plurality of wells includes obtaining an upper portion of the microplate. A bottom surface of the microplate is coated with a bottom surface uncured resin. A sheet material is disposed adjacent a central portion of the bottom surface. A frame is disposed adjacent an edge portion of the bottom surface. The frame contains a frame uncured resin. At least a portion of the bottom surface uncured resin is cured to produce a bottom surface cured resin. At least a portion of the frame uncured resin is cured to produce a frame cured resin. A remaining portion of the bottom surface uncured resin is removed subsequent to curing at least a portion of the bottom surface uncured resin.

Function fabrication in a microfluidic device manufactured with a custom 3D printer. The functions may include, for example, transporting or routing fluid, fluid mixing through flow and/or diffusion, blocking fluid (valve), pumping fluid, providing chemical reaction regions, providing analyte capture regions, and providing analyte separation regions. The fluid may be a liquid or a gas.