The present disclosure relates to a method of manufacturing a microfluidic device, which may precisely form a channel having a desired shape within one substrate using a wax regardless of a shape of a hydrophilic porous substrate, and more specifically, to a method of manufacturing a microfluidic device in which a microchannel is formed by a wax within one hydrophilic porous substrate, the method including: an operation of stacking and then heat-treating a transfer film on which a mirror image of a wax pattern is formed to form a microchannel and the substrate.

The present invention provides a diagnostic kit for detecting the presence or quantity of one or more test analytes within a test sample taken from a body surface of a mammal, the diagnostic kit comprising: a separate insert for a lateral flow device (200, 411) comprising a membrane (201) fixed to a rigid support (202) and, the separate insert being configured to obtain the test sample; a lateral-flow assay device configured (300, 400) to accept the separate insert (200, 411); a securing member (210) configured to releasably attach (211) the separate insert to a body surface of a mammal (213); wherein the securing member (210) comprise an expandable layer (212) configured to apply pressure to the separate insert (200, 411) thereby pressing the separate insert (200, 411) against the body surface of the mammal (213).

A kit for detecting a nucleic acid and a method for preparing the same are provided. The kit includes: a test area which has a lower surface formed as a concave curved surface and includes molecular beacons concentrated on a glass fiber network; a surrounding area which completely surrounds the test area; and a support area which surrounds the surrounding area, wherein the surrounding area includes a hydrophobic polymer on the glass fiber network, the test area does not include the hydrophobic polymer, and a glass fiber in the test area is functionalized.

A device configured to collect a blood sample comprising a capillary means, wherein the capillary means is configured to collect and dry the blood sample and comprises an effective amount of a distributed inhibitor of phospholipase D. The device may be configured to receive, transport and collect a blood sample comprising a compartment in fluid connection with the capillary means, wherein the capillary means is configured to collect and dry the blood sample and comprises an effective amount of a distributed PLD inhibitor. The device may be a microfluidic device comprising an inlet portion, an outlet portion comprising a capillary means configured to collect and dry the blood sample, and optionally a metering function, wherein the microfluidic device comprises an effective amount of a distributed PLD inhibitor. The PLD inhibitor is distributed in a water soluble film, preferably a PVA film, or in an absorbent paper or polymer or in the capillary means. The PLD inhibitor may be selected from a salt of a transition metal belonging to column 5 or 6 of the periodic table, a salt of vanadium and a salt of tungsten, a salt comprising a vanadium oxyanion and a salt comprising a tungsten oxyanion, and/or at least one of NaVO3 (sodium metavanadate) and Na2WO4 (sodium tungstate). A method of preparing a sample for analysis of phosphatidylethanol (PEth) comprises providing a blood sample with a volume of less than 10 ml to the device; contacting the blood sample with at least one inhibitor of the enzyme phospholipase D selected from at least one of a salt of vanadium and a salt of tungsten; and admitting inhibition of phospholipase D so formation of PEth is blocked.

A test strip for sampling a bodily fluid may include multiple layers of a substrate material, an adhesive between at least some of the multiple layers, and a microfluidic channel formed between at least some of the multiple layers. The test strip may further include multiple electrodes on one of the multiple layers, positioned and partially exposed within the microfluidic channel, an additional material positioned at or near an entrance to the microfluidic channel, to selectively limit the flow of at least one of bubbles or debris into the microfluidic channel, and at least one exit port in at least one of the multiple layers to allow for release of pressure from the test strip. In some embodiments, the test strip is a saliva analysis test strip. In some embodiments, the test strip includes multiple exit ports to prevent blockage of sample flow.

The present invention is directed to a foldable transport container suitable to receive and hold multiple plates with samples in it. The container is comfortable to load, transport and unload. The invention also relates to a strip of material for providing such a foldable transport container. Finally the invention relates to a method for assembling such a foldable container.

The present invention relates to a method for manufacturing structural layers for guiding liquid flow on a porous substrate, by printing onto at least one area of at least one surface of the substrate a printing solution containing an aqueous dispersion of a poly(lactic acid)-based copolymer.

This present disclosure provides devices, systems, and methods for performing point-of-care analysis of a target analyte in a biological fluid via a binding assay. The present disclosure includes a cartridge for collecting the target analyte contained in a fluid sample and performing an assay. The cartridge includes an assay stack having a first separation layer, a second separation layer, and a detection membrane. The cartridge also includes a plurality of first complexes comprising a capture molecule and a magnetic bead and a plurality of second complexes comprising a detection molecule and a detection label. Further, the detection membrane includes a substrate that interacts with the detection label to elicit a quantifiable response in the presence of the target analyte. The quantifiable response corresponds to an amount of detection antibody present in the detection membrane, and the amount of detection antibody present corresponds to an amount of the target analyte present.

A portable diagnostic device for performing the diagnosis of pathogens, such as viruses, bacteria, microorganisms, etc., by rapidly detecting their nucleic acids in a biological sample to be tested. Also, the use of the portable diagnostic device and the methods for detection of at least one nucleic acid sequence of interest implemented with the aid of the portable diagnostic device.

The present invention describes a blotting material comprising a profiled region that has a portion configured to protrude at least partially into a space created within a boundary formed by the rim of a holder for a sample grid. Also described are methods for making such profiles and components used to make said profiles. There are also provided methods for removing excess liquid from a sample grid by bringing the profiled blotting material into association with the sample grid past the sample grid holder. Systems comprises means to hold a sample grid holder, means to hold the blotting paper, and means to bring the two together are also described.