A microfluidic chip configuration wherein injection occurs in an upwards vertical direction, and fluid vessels are located below the chip in order to minimize particle settling before and at the analysis portion of the chip's channels. The input and fluid flow up through the bottom of the chip, in one aspect using a manifold, which avoids orthogonal re-orientation of fluid dynamics. The contents of the vial are located below the chip and pumped upwards and vertically directly into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. Then the channel takes a short horizontal turn that nearly negates any influence of cell settling due to gravity and zero flow velocity at the walls. The fluid is pumped up to a horizontal analysis portion that is the highest channel/fluidic point in the chip and thus close to the top of the chip, which results in clearer imaging. A laser may also suspend cells or particles in this channel during analysis which prevents them from settling.

The present exemplary embodiments provide a sample separation device which applies an electric field to a selective ion permeable layer based on origami to concentrate a target material in a specific area and concentrates a target material and separates a non-target material through a filter layer in which a paper is compressed to adjust a size of micro pore.

A fluidic device for processing a fluid or species therein is described. The device comprises a 3D channel including an inlet for receiving a sample fluid and an outlet for outputting the sample fluid. The channel is adapted for guiding flow of the sample fluid in an axial direction from the inlet to the outlet. The channel includes at least two side walls. The device also has a controllable flow inducer having electrodes for inducing, when the sample fluid is flowing through the channel, a motion of the sample fluid in the channel in a plane substantially orthogonal to the axial direction. Along at least one of the side walls at least part of the electrodes are formed by alternatingly at least an electrically conducting portion, an electrically insulating portion and a further electrically conducting portion.

Devices that may couple two or more apparatuses, such as an organ-on-a-chip device and a microfluidic device. Devices that include an organ-on-a-chip device, a microfluidic device, and a cap that couples the organ-on-a-chip device and the microfluidic device. Systems that include the devices and a detection unit. Methods for quantitation of insulin.

Described herein is a size-based asymmetric nanopore membrane (ANM) filtration technology for high-efficiency exosome isolation, concentration, and fractionation. The ANM design prevents exosome deformation, lysing, and fusion due to the strong external force and thus significant increases the yield (up to 92%) while preserving other advantages of size-based ultrafiltration. It also offers a unique feature of being able to flush the contaminating proteins from the exosomes. It offers higher throughput, yield, sample purity, concentration factor, and more precise size fractionation than current approaches.

There is provided a system and method for angstrom confinement of trapped ions. The method including: receiving water molecules and ionic compounds in a first reservoir, an angstrom confinement assembly is positioned between the first reservoir and a second reservoir, the angstrom confinement assembly defining angstrom conduits; and repeatedly applying an electric field across a first electrode and a second electrode, the first electrode on a same side of the angstrom confinement assembly as the first reservoir and the second electrode on a same side of the angstrom confinement assembly as the second reservoir, the electric field applied such that, when the electric field is applied, positive ions of the ionic compounds are induced to flow through the angstrom conduits, and wherein, when the electric field is not applied, water molecules flow into the angstrom conduits due to capillary forces to confine the positive ions in the angstrom conduits.

An electro-osmosis pump system includes an inlet line through which a fluid is introduced, an outlet line through which the fluid is discharged, a first pump disposed between the inlet line and the outlet line and including a first housing in which a first operation fluid is disposed, a second pump disposed in parallel to the first pump between the inlet line and the outlet line and including a second housing in which a second operation fluid disposed, and a power supply configured to supply voltages to the first pump and the second pump. The first pump includes a first membrane, a 1A-th electrode, and a 2A-th electrode, the second pump includes a second membrane, a 1B-th electrode, and a 2B-th electrode, and the power supply supplies the voltage to the 1A-th electrode and the 2A-th electrode, and supplies the voltage to the 1B-th electrode and the 2B-th electrode.

The present invention relates to the technical field of microfluidics, and specifically relates to an electroosmotic micropump apparatus and an electroosmotic micropump apparatus group. The electroosmotic micropump apparatus in the present invention comprises fluid micro channels and a microneedle electrode; each fluid micro channel is used for communicating a micro flow channel inlet with a micro flow channel outlet for pumping a fluid; the microneedle electrode comprises a first microneedle type electrode and a second microneedle type electrode that are respectively provided at the micro flow channel inlet and the micro flow channel outlet; the first microneedle type electrode and the second microneedle type electrode are oppositely arranged; moreover, neither of the first microneedle type electrode and the second microneedle type electrode is in conduction with the fluid micro channel. The electroosmotic micropump apparatus of the present invention can provide a parallel and uniform electric field for the interior of the fluid micro channel and generate a stable electroosmotic driving force, and can solve the hydrolysis problem of the surface of an electrode, thereby greatly improving the stability of the running of a micropump and prolonging the service life of the micropump.

Methods, systems and devices that allow independently applied pressures to a BGE reservoir and a sample reservoir for pressure-driven injection that can inject a discrete sample plug into a separation channel that does not require voltage applied to the sample reservoir and can allow for in-channel focusing methods to be used. The methods, systems and devices are particularly suitable for use with a mass spectrometer.