The present disclosure is directed to methods and devices for reducing or otherwise mitigating accumulated reagent material and/or fluids within a dispense nozzle of a dispenser.

The invention relates to a substrate for testing samples, in particular cells or molecules, wherein the substrate comprises a fluid system comprising a sample chamber configured in the substrate for storing and testing samples and at least one liquid reservoir in fluid communication with the sample chamber, and wherein the substrate comprises a passive blocking element capable of assuming a closed position and an open position, wherein in the closed position a fluid exchange between the sample chamber and the liquid reservoir is blocked.

The microfluidic device capable of initiating sequential flow according to the present invention includes: a main flow path in which a suction port for sucking the fluid with a negative pressure is formed at one end; a plurality of reservoirs that supply a fluid stored therein to the main flow path through an outlet by the negative pressure applied to the suction port, and are connected to a plurality of different points of the main flow path; and a blocking element that blocks the inflow of external air to the main flow path through the outlet when all the fluid in the reservoir flows out, wherein the fluid stored in a plurality of the reservoirs may flow sequentially.

The present disclosure is directed to methods and devices for reducing or otherwise mitigating accumulated reagent material and/or fluids within a dispense nozzle of a dispenser.

In one aspect of the invention, the fluidic device includes a fluidic chip includes a body having a first surface and an opposite, second surface, one or more channels formed in the body in fluidic communications with input ports and output ports for transferring one or more fluids between the input ports and the output ports, and a fluidic chip registration means formed on the first surface for aligning the fluidic chip with a support structure; and an actuator configured to engage with the one or more channels at the second surface of the body for selectively and individually transferring the one or more fluids through the one or more channels from at least one of the input ports to at least one of the output ports at desired flowrates.

This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

An analytical device for automated determining of a measured variable of a liquid sample, includes: a base module; a replaceable cassette connectable with the base module and having at least one liquid container connectable via a fluid line with a measuring cell and containing a reagent to be added to the liquid sample for forming a measured liquid; and a measuring transducer for registering measured values correlated with the measured variable for the measured liquid accommodated in the measuring cell. The cassette has, associated with the at least one liquid container, a fluid coupling apparatus having a primary component and a secondary component and serving for producing a connection of the fluid line with the liquid container.

Multi-component separation devices configured to separate components of a liquid sample by centrifugation are provided. Aspects of the separation devices include a container having a distal end and a proximal end and a buoy configured to be displaced along a longitudinal axis within the container where the buoy includes one or more sealed chambers. Also provided are methods of using the subject devices to separate components of a multi-component liquid sample such as whole blood, bone marrow aspirate or stromal vascular fraction as well as systems suitable for practicing the subject methods.

A valve block assembly for a bottle attachment apparatus for handling liquids has a valve block, an intake valve insert and/or an exhaust valve insert, and a valve receptacle assigned to the respective valve insert. The valve insert is inserted in a replaceable and sealing manner in the valve receptacle. The valve insert has a stop portion, a fastening portion distinct therefrom and a sealing portion distinct from both. The associated valve receptacle has stop, fastening, and sealing portions matching the equivalent portions of the valve insert. When the valve insert is installed correctly both the stop portions and the sealing portions bear on one another in a planar manner.

A fluid dispensing device includes a dispensing cylinder, a first inlet and a second inlet. The first inlet is disposed within a first container on which the fluid dispensing device is mounted. The first inlet and the second inlet are configured to facilitate intake of only one fluid into the dispensing cylinder at a given point in time. The fluid dispensing device also includes a first outlet and a second outlet. The first outlet and the second outlet are configured to dispense only one fluid out of the dispensing cylinder at a given point in time. The fluid dispensing device further includes a valve assembly fluidically coupled to the first inlet and the second inlet, and to the first outlet and the second outlet. The valve assembly is configured to control flow of the fluids within and/or out of the dispensing cylinder.