A fluidic device having first side and second side. The fluidic device includes first latch mechanism and second latch mechanism for receiving connector, arranged on first side; and a first fluidic chip holder and second fluidic chip holder, arranged on first side in alignment with first latch mechanism and second latch mechanism, for holding first fluidic chip and second fluidic chip, respectively. The fluidic device includes a traction surface to couple the fluidic device with an actuator to move the fluidic device to select which of first or second fluidic chip is to be used. Disclosed is an apparatus with a container, tubular material feeding line, tubular printing composition feeding line, and actuator.

There is provided a sample processing cartridge comprising a. a sample entry location; b. a closed sample processing chamber; c. a sample analysis location comprising a sample analysis well; d. a first channel fluidly connecting the sample entry location and the sample processing chamber; e. a second channel connecting the sample analysis location and the sample processing chamber, the second channel comprising a closed or closable second channel valve; wherein the sample processing chamber comprises a second channel port providing fluid connection between the second channel and the sample processing chamber, the second channel port being positioned in a sample accumulating region of the sample processing chamber. There is also provided a sample processing system comprising the cartridge, and methods of use of the cartridge and processing system in a sample processing assay.

An integrated microfluidic chip and a single-cell culture, screening, and export method applying the same are disclosed; the chip includes a base, an inlet flow channel, an outlet flow channel, a plurality of common flow channels and a plurality of functional units, wherein two ends of the common flow channel are connected to the inlet flow channel and the outlet flow channel, respectively, wherein each of the functional units includes a single-cell introduction port, a cell culturing-screening chamber, a cell export chamber, a cell export port, and a drive element, wherein the drive element is used to provide power to liquid to introduce single cells entering the common flow channels into the cell culturing-screening chamber, and after culturing and screening, to export target cell population in the cell culturing-screening chamber through the cell export port.

Devices and methods for determining activity of one or more coagulation factors in a blood sample are provided. The device may comprise an inlet port for deposition of a sample, a reaction compartment, a detection compartment, a control compartment, or any combination thereof. One or more compartments may be fluidically connected. One or more compartments may comprise plasma deficient of a coagulation factor, an ionic citrate source, an ionic calcium source, one or more coagulation contact phase activator reagents, a phospholipid, or a mixture, or any combination thereof.

Provided herein is a valve manifold comprising (a) an elastomer sheet attached to a plurality of magnetic pistons, wherein the magnetic pistons project from a first side of the elastomer sheet; (b) a foot component comprising a first surface and a plurality of shafts that orthogonally pass through the first surface; and (c) a body component comprising a second surface, a groove that laterally passes along the second surface, and a plurality of reservoir channels that orthogonally pass through the second surface, wherein the elastomer sheet is compressed between the foot component and the body component.

Instrument for performing a diagnostic test on a fluidic cartridge A cartridge reader is for carrying out a diagnostic test on a sample contained in a fluidic cartridge inserted into the reader. The fluidic cartridge comprises a fluidic layer comprising at least one sample processing region, at least one collapsible blister containing a liquid reagent, a pneumatic interface, an electrical interface and at least one mechanical valve. The reader comprises a housing; an upper clamp occupying a fixed position relative to the reader, and a lower clamp, movable relative to the first clamp, wherein the upper clamp and the lower clamp define a cartridge receiving region therebetween. The reader comprises a thermal module comprised in the lower clamp, wherein the thermal module comprises at least one thermal stack for heating the at least one sample processing region of the cartridge inserted into the reader. The reader comprises at least one mechanical actuator for actuating the mechanical valve comprised in the cartridge inserted into the reader.

Methods, systems and devices that allow independently applied pressures to a BGE reservoir and a sample reservoir for pressure-driven injection that can inject a discrete sample plug into a separation channel that does not require voltage applied to the sample reservoir and can allow for in-channel focusing methods to be used. The methods, systems and devices are particularly suitable for use with a mass spectrometer.

There is provided a sample processing cartridge comprising a. a sample entry location; b. a closed sample processing chamber; c. a sample analysis location comprising a sample analysis well; d. a first channel fluidly connecting the sample entry location and the sample processing chamber; e. a second channel connecting the sample analysis location and the sample processing chamber, the second channel comprising a closed or closable second channel valve;
wherein the sample processing chamber comprises a second channel port providing fluid connection between the second channel and the sample processing chamber, the second channel port being positioned in a sample accumulating region of the sample processing chamber.

There is also provided a sample processing system comprising the cartridge, and methods of use of the cartridge and processing system in a sample processing assay.

Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids.

A tubing-free, sample-to-droplet microfluidic system includes a tubing-free, sample-to-droplet microfluidic chip; a valve control system connected to the tubing-free, sample-to-droplet microfluidic chip; a vacuum system fluidly connected to the tubing-free, sample-to-droplet microfluidic chip; and a droplet formation pressure system fluidly connected to the tubing-free, sample-to-droplet microfluidic chip. A microfluidic chip for a tubing-free, sample-to-droplet microfluidic system includes a tubing-free, sample-to-droplet interface section; a droplet mixing section in fluid connection with the tubing-free, sample-to-droplet interface section to received droplets therefrom; an incubation section in fluid connection with the droplet mixing section to receive droplets therefrom; and a detection section in fluid connection with the incubation section to receive droplets therefrom.