Techniques regarding one or more structures that can facilitate automated, multi-stage processing of one or more nanofluidic chips are provided. For example, one or more embodiments described herein can comprise a system, which can comprise a roller positioned adjacent to a microfluidic card comprising a plurality of fluid reservoirs in fluid communication with a plurality of nanofluidic chips. An arrangement of the plurality of nanofluidic chips on the microfluidic card can defines a processing sequence driven by a translocation of the roller across the microfluidic card.

An apparatus includes a flow cell body, a plurality of electrodes, an integrated circuit, and an imaging assembly. The flow cell body defines one or more flow channels and a plurality of wells. Each flow channel is configured to receive a flow of fluid. Each well is fluidically coupled with the corresponding flow channel. Each well is configured to contain at least one polynucleotide. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are operable to effect writing of polynucleotides in the corresponding wells. The integrated circuit is operable to drive selective deposition or activation of selected nucleotides to attach to polynucleotides in the wells to thereby generate polynucleotides representing machine-written data in the wells. The imaging assembly is operable to capture images indicative of one or more nucleotides in a polynucleotide.

Cell separation systems, and methods for separating cells from microcarriers, and harvesting the separated cells, are provided, wherein the system comprises a cell separation device, a cell settling device, and a cell screening device.

In an electrochemical lateral flow immunological test method, flow of a sample solution is controlled. As a result, the reaction time is short and quantitative measurements and electrical measurements can be performed with excellent sensitivity and high accuracy, and the invention provides a sensor employed in the method. Electrode portions, electrically conductive portions for transferring electric current from the electrode portions, and connecting portions connected to an electrical measuring instrument for measuring the electric current values are arranged on a supporting body including a resin sheet, pads and the like disposed by partial lamination on the supporting body. A sample solution flows over the plurality of pads, and electrochemical detection is performed by controlling the flow at the position of the electrode portions. Furthermore, the flow is controlled by a flow rate control pad, a flow passage portion fiber pad, and flow rate control protruding portions.

A microfluidics device has one or more bubble diversion regions. Problems associated with the generation of air bubbles are avoided in a microfluidics device such as a cartridge, for use with a point of care (POC) diagnostics device, the cartridge being able to carry out downstream processing such as polymerase chain reaction (PCR) and/or nucleic acid capture. The bubble diversion region has a lower flow resistance than the flow resistance of an area of interest.

Techniques regarding one or more structures that can facilitate automated, multi-stage processing of one or more nanofluidic chips are provided. For example, one or more embodiments described herein can comprise a system, which can comprise a roller positioned adjacent to a microfluidic card comprising a plurality of fluid reservoirs in fluid communication with a plurality of nanofluidic chips. An arrangement of the plurality of nanofluidic chips on the microfluidic card can defines a processing sequence driven by a translocation of the roller across the microfluidic card.

In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.

Provided is a chip that does not require high-accuracy discharge amount control for a liquid delivery pump and can suppress the entrainment of air bubbles. A chip 1 for test or analysis is provided with a flow path 4 through which a fluid is delivered, the chip 1 including: a first flow path 5 through which a first fluid is delivered; a second flow path 6 through which a second fluid is delivered; a merging portion 8 configured to be provided on a downstream end portion 5a side of the first flow path 5 and merge the first fluid and the second fluid; a first connection flow path 9 configured to connect the first flow path 5 and the second flow path 6 at the merging portion 8 and have a liquid delivery resistance higher than a liquid delivery resistance of the first flow path 5; a degassing flow path 13 configured to be connected to the second flow path 6 on a downstream side of the first connection flow path 9; a third flow path 7 configured to be provided on a downstream side of the merging portion 8; and a second connection flow path 10 configured to connect the first flow path 5 and the third flow path 7 and have a liquid delivery resistance higher than the liquid delivery resistance of the first flow path 5.

An apparatus for performing microfluidic-based biochemical assays, the apparatus includes a microfluidic device, wherein the microfluidic device comprises at least a microfluidic feature comprising at least a reservoir configured to contain at least a fluid, and at least an alignment feature for positioning and attaching a sensor device, wherein the at least an alignment feature is not contacting the at least a microfluidic feature, at least a sensor device configured to be in sensed communication with the at least a fluid and detect at least a sensed property, and at least a flow component fluidically connected to the at least a microfluidic feature configured to flow the at least a fluid through the at least a sensor device.

Aspects of this disclosure relate to systems that include a channel with at least one fluid in particle in fluid. The at least one particle can move along a defined path of the channel in response to a magnetic field. At least one structure is integrated with the channel, such as a sensor to generate an output signal related to the magnetic field or a magnetic structure to apply the magnetic field. Relates methods are also disclosed.