A microfluidic device comprises a lower layer that is electrically conductive and transparent with respect to an incident optical beam, an upper layer, comprising first portions that are electrically conductive and second portions that are electrically insulating, adjacent and alternated to the first ones; a compartment seamlessly extending between the lower layer and the upper layer; the compartment contains a filler medium configured to emit an optical emission beam and markers dispersed in the filler medium, which are electrically charged and are adapted to move inside the compartment in all directions according to the intensity of the electrical signal applied to the first portions, the filler medium is configured to interact with the markers to increase or decrease the intensity of the optical emission beam according to the local concentration of the markers.

Methods for on-demand printing discrete entities including, e.g., cells, media or reagents to substrates are provided. In certain aspects, the methods include manipulating qualities of the entities or biological components thereof. In some embodiments, the methods may be used to create arrays of microenvironments and/or for two and three-dimensional printing of tissues or structures and/or for in situ printing for microsurgeries. Systems and devices for practicing the subject methods are also provided.

A sample analysis substrate mountable and detachable to a sample analysis device and includes: a plate-shaped base substrate; and a chamber, the chamber being a space in which to cause a binding reaction, The sample analysis device includes: a motor to rotate the sample analysis substrate; a first magnet unit to attract the magnetic particles; a first actuator to move the first magnet unit to change relative positions of the first magnet unit and the sample analysis substrate; and a control circuit to control the motor, the drive circuit, and the first actuator. The first magnet unit shaped as a whole shape or a partial shape of a circle or a ring. During a B/F separation for separating reacted substance from unreacted substance, the first actuator moves the first magnet unit to a position where the magnetic particles in the chamber are attracted by the first magnet unit.

A particle arrangement transportation device includes: a base material in which is formed a flow channel from an inlet port-side opening through which a solution containing particles to be an object of arrangement and transportation is introduced to an outlet port-side opening; an electrode which is formed along the flow channel on a wall surface of the base material being exposed in the flow channel; electrodes which are formed along the flow channel in the base material on both sides of the flow channel; and a power supply which applies an AC voltage between the electrodes.

There is provided a biological substance detection chip having high detection accuracy. The present technology provides a biological substance detection chip which is composed of a plurality of pixels in which the pixel includes at least a holding surface on which a biological substance is held and a photoelectric conversion unit that is provided below the holding surface and provided on a semiconductor substrate, wherein a partition wall made of a conductor is provided between the pixels on the holding surface. In addition, the present technology provides a biological substance detection device and a biological substance detection system using the biological substance detection chip.

Technologies for acoustoelectronic manipulation of micro/nano particles include a system having a piezoelectric substrate coupled to one or more acoustic transducers and a fluid layer positioned above the substrate. Micro/nano particles are introduced to the fluid, which can be in the form of a droplet or in a confined channel, and a signal is applied to the acoustic transducer. One or more parameters of the signal are varied after introducing the micro/nano particles into the fluid. The parameters may include amplitude, frequency, or phase of the signal. The system may include one or more acoustic transducers. Multiple signals may be applied to the acoustic transducers. Wave superposition of acoustic waves in the substrate manipulates micro/nano particles in the fluid. The nanoparticles may include carbon nanotubes, nanowires, nanofibers, graphene flakes, quantum dots, SERS probes, exosomes, vesicles, DNA, RNA, antibodies, antigens, macromolecules, or proteins.

The invention relates to a system (10) adapted to measure multiple biophysical characteristics of cells, the system (10) comprising: a microfluidic chip (12) provided with a microfluidic channel (14) which allows cells to flow through, the microfluidic channel (14) having an inlet (14a), an outlet (14b), and a lateral opening (14c) situated between the inlet (14a) and the outlet (14b); and a capacitive sensor (30) integrated in the microfluidic chip, adapted to obtain biophysical characteristics of a single cell in the microfluidic channel (14) by directly manipulating the single cell by sensor elements (31, 32) through the lateral opening (14c) of the microfluidic channel (14), the sensor (30) comprising a stationary part and an electrostatically driven movable part which is movable relative to the stationary part, the stationary part being fixed to the microfluidic chip (12), the movable part being arranged in the lateral opening (14c) of the microfluidic channel (14), wherein a portion of the sensor elements (31, 32) provides an interface between fluid and air in the system.

Disclosed herein is a system to detect and characterize individual particles and cells using at least either optic or electric detection as the particle or cell flows through a microfluidic channel. The system also provides for sorting particles and cells or isolating individual particles and cells.

Apparatus and method for separating whole cells from a mixture, e.g., including liquid, other cell types, nucleic acid material, or other components. Focused acoustic energy may be used to move whole cells in a chamber so that the cells exit the chamber via a first outlet rather than a second outlet. A filter may, or need not, be used to assist in separation.

Acoustofluidic systems including acoustic wave generators for manipulating fluids, droplets, and micro/nano objects within a fluid suspension and related methods are disclosed herein. According to an aspect, an acoustofluidic system includes a substrate including a substrate surface. The system also includes an acoustic wave generator configured to generate acoustic streaming within an acoustic wave region of the substrate surface. Further, the acoustic wave generator is controllable to change the acoustic streaming for movement of a droplet or other micro/nano object on a fluid suspension about the acoustic wave region.