Methods, devices and systems for analyzing precious samples of cells, including single cells are provided. The methods, devices, and systems in various embodiments of the invention are used to assess genomic heterogeneity, which has been recognized as a central feature of many cancers and plays a critical role in disease initiation, progression, and response to treatment. The methods devices and systems are also used to analyze embryonic biopsies for reimplantation genetic diagnosis (PGD). In one embodiment, the devices, systems and methods provided herein allow for the construction of genomic and RNA-seq libraries without a pre-amplification step.

Embodiments described herein relate to an apparatus for positioning and securing an excised biological tissue specimen for imaging and analysis. In some embodiments, an apparatus includes a sample bag defining an inner volume configured to receive a biological tissue sample, and a sealing member coupled to the sample bag. An imaging window is disposed and configured to be placed in contact with at least a portion of the biological tissue sample, and a positioning member is coupled to the imaging window and is configured to be disposed against the sealing member to substantially seal the inner volume. The positioning member includes a vacuum port disposed and configured to be aligned with a vacuum source to withdraw air from the inner volume of the sample bag.

The invention relates to a sealed container (1) comprising a flange (5, 6) that delimits a through-passage having an axial axis in the container, a door (7) movably mounted on the flange (5, 6), and a first annular seal (13) located between the flange (5, 6) and the door (7), the flange, the door and the first seal forming a sealed connection device that can be connected to a complementary connection device on a sterile enclosure by bayonet connection means in order to allow sterile communication between the sealed container and the sterile enclosure, characterized in that the container (1) includes a protective cover (20) on the connection device, said protective cover being forcibly clipped onto the container, and in that the container further includes contact pressure means arranged between the protective cover (20) and the connection device so as to apply contact pressure to the connection device in the axial direction proportional to the clipping force.

A body fluid analyte detection device, includes: a transmitter, provided with at least one first clamping part; a bottom shell, provided with a second clamping part corresponding to the first clamping part, the transmitter being assembled on the bottom shell by mutual snap fitting of the first clamping part and the second clamping part, the bottom shell including a fixing part and a force application part, and the fixing part being fixed and applying a force to the force application part in a direction to disable the bottom shell, so that the first clamping part and the second clamping part are separated from each other, and then the bottom shell and the transmitter are separated; a battery, used for supplying power to the transmitter; a sensor, used for detecting body fluid analyte parameter information and electrically connected to the transmitter to transmit a parameter signal.

An aseptic bioprocess package is provided herein. The aseptic bioprocess package includes a 2D flexible container including an interior compartment, a height having an upper half and a lower half, an inlet and an outlet, the inlet and the outlet being disposed on the same half of the 2D flexible container and a channel-forming feature in the interior compartment of the container, the channel-forming feature being configured to maintain a fluid flow path that fluidly connects the interior compartment of the flexible container with the outlet.

The invention relates to a sealed container (1) comprising a flexible film (2) which is arranged to define an internal space (3) and a through-opening, and a sealed connection device (4) which is arranged to allow the flexible film (2) to be connected to a complementary connection device of a sterile enclosure, while ensuring sterile communication between the internal space (3) of the container (1) and that of the enclosure, the connection device (4) comprising a flange to which the flexible film (2) is attached, the flange delimiting the through-opening of the flexible film (2), characterized in that the flange is constituted by two different pieces distinct from each other, respectively forming an external sleeve (5) and an internal sleeve (6) the flexible film (2) being kept gripped between the external sleeve (5) and the internal sleeve (6).

Herein disclosed are biorelevant dissolution media using a phosphonocholic acid salt in combination with a polysorbate. This tailor-made combination of surface-active agents apparently forms mixed micelles similar to those formed in the currently available biorelevant media, is faster and easier to prepare, forms reproducible and consistent dissolution media, and compares well with the predictive properties of currently available biorelevant dissolution media and thus is useful to predict the in vivo behavior of dosage forms of poorly water-soluble drugs.

Devices and methods for immunomagnetic separation having a flexible inflatable processing chamber and magnetic array.

A trace analyte collection swab having a collection surface at least partially coated with a microscopically tacky substance to enhance pick-up efficiency is described. In embodiments, the truce analyte collection swab comprises a substrate including a surface having a trace analyte collection area and a coating disposed on the surface of the substrate in the trace analyte collection area. The coating is configured to be microscopically adhesive to collect particles of the trace analyte from a surface when the trace analyte collection area is placed against the surface. In one embodiment, the coating comprises Polyisobutylene.

A module for processing fluids includes one or more collapsible fluid chambers supported on a substrate, a compression element supported on the substrate and configured to be movable with respect to the one or more chambers, and an actuating element coupled to the compression element and configured to effect movement of the compression element relative to the one or more fluid chambers to collapse each fluid chamber by compressing the fluid chamber between the compression element and the substrate as the compression element moves over the fluid chamber. A method for motivating a fluid out of a fluid chamber comprises the steps of providing a module that includes one or more collapsible fluid chambers supported on a substrate, a compression element supported on the substrate and configured to be movable with respect to the one or more chambers, and an actuating element coupled to the compression element and configured to effect movement of the compression element relative to the one or more fluid chambers and moving the actuator element to move the compression element across at least a portion of t substrate and compress the fluid chamber, thereby motivating the fluid out of the fluid chamber.