A system is provided for the quantitative magnetic separation of magnetic objects (e.g., particles or cells). The system uses magnetic ratcheting over arrays of ferromagnetic elements having gradient spacing manifested in various pitch zones that are encountered by the magnetic objects as they traverse the array. The system can be used to separate and concentrate magnetic objects based on iron oxide content. For cells, different phenotypes may be separated based, for example, on surface expression of proteins or molecules that are bound to magnetic particles. The system includes a substrate or chip having the array of ferromagnetic elements with increasing lateral pitch and an externally driven magnet device that generates a cycling magnetic field. Magnetic objects with higher IOC separate and equilibrate along the array at larger pitches. The system can be used for the differential sorting of particles and cells of interest.

A system is provided for the quantitative magnetic separation of magnetic objects (e.g., particles or cells). The system uses magnetic ratcheting over arrays of ferromagnetic elements having gradient spacing manifested in various pitch zones that are encountered by the magnetic objects as they traverse the array. The system can be used to separate and concentrate magnetic objects based on iron oxide content. For cells, different phenotypes may be separated based, for example, on surface expression of proteins or molecules that are bound to magnetic particles. The system includes a substrate or chip having the array of ferromagnetic elements with increasing lateral pitch and an externally driven magnet device that generates a cycling magnetic field. Magnetic objects with higher IOC separate and equilibrate along the array at larger pitches. The system can be used for the differential sorting of particles and cells of interest.

A device for separating a sample of cells suspended in a bio-compatible ferrofluid is described, The device includes a microfluidic channel having a sample inlet, at least one outlet and a length between the same inlet and the at least one outlet, wherein a sample can be added to the sample inlet and flow along the microfluidic channel length to the at least one outlet. The device includes a plurality of electrodes and a power source for applying a current to the plurality of electrodes to create a magnetic field pattern along the microfluidic channel length. The present invention also includes a method of using said device for separating at least one cell type.

A device for separating a sample of cells suspended in a bio-compatible ferrofluid is described, The device includes a microfluidic channel having a sample inlet, at least one outlet and a length between the same inlet and the at least one outlet, wherein a sample can be added to the sample inlet and flow along the microfluidic channel length to the at least one outlet. The device includes a plurality of electrodes and a power source for applying a current to the plurality of electrodes to create a magnetic field pattern along the microfluidic channel length. The present invention also includes a method of using said device for separating at least one cell type.

An electromagnetic pulsed-wave system having an electromagnetic boom for generating a time-varying pulsed-wave to control a colloidal mixture disposed in water and a depository. The electromagnetic boom comprising a plurality of electrically coupled solenoids disposed at the water for providing electromagnetic pulses to generate the time-varying pulsed-wave to transport the colloidal mixture. The depository having an electromagnetic ramp magnetically coupled with the electromagnetic boom and a separation receptacle for separating magnetized particles from the colloidal mixture.

An electromagnetic pulsed-wave system having an electromagnetic boom for generating a time-varying pulsed-wave to control a colloidal mixture disposed in water and a depository. The electromagnetic boom comprising a plurality of electrically coupled solenoids disposed at the water for providing electromagnetic pulses to generate the time-varying pulsed-wave to transport the colloidal mixture. The depository having an electromagnetic ramp magnetically coupled with the electromagnetic boom and a separation receptacle for separating magnetized particles from the colloidal mixture.

Devices, methods, and systems are provided for extracting particles from a ferrofluid. Such methods may comprise receiving a flow of ferrofluid comprising target particles and background particles and generating a first, focusing magnetic field to focus the target particles towards a capture region. The capture region may capture the target particles and a plurality of background particles. A second, defocusing magnetic field may be configured to remove background particles from the capture region. A detector may be used to detect the target particles bound to the target region.

Devices, methods, and systems are provided for extracting particles from a ferrofluid. Such methods may comprise receiving a flow of ferrofluid comprising target particles and background particles and generating a first, focusing magnetic field to focus the target particles towards a capture region. The capture region may capture the target particles and a plurality of background particles. A second, defocusing magnetic field may be configured to remove background particles from the capture region. A detector may be used to detect the target particles bound to the target region.

A device and method for extracting particles contained in a ferrofluid medium are provided. Such methods may comprise suspending particles of different sizes in a ferrofluid medium and containing the ferrofluid medium in a cylindrical reservoir, and applying a first magnetic field to at least a portion of the reservoir. The first magnetic field is configured to indirectly exert a force on at least a portion of the particles of a predetermined size, and direct the portion of particles in a desired direction.

A device and method for extracting particles contained in a ferrofluid medium are provided. Such methods may comprise suspending particles of different sizes in a ferrofluid medium and containing the ferrofluid medium in a cylindrical reservoir, and applying a first magnetic field to at least a portion of the reservoir. The first magnetic field is configured to indirectly exert a force on at least a portion of the particles of a predetermined size, and direct the portion of particles in a desired direction.