Disclosed herein are methods for isolating target cells from blood, involving mixing in an open container an undiluted blood sample having a volume of 10 ml or less, and binding agents, wherein each binding agent comprises (A) a primary binding agent comprising an agent capable of binding to at least one cellular epitope on target cells in the undiluted blood sample, (B) a first linker bound to the primary binding agent, to generate binding agent-attached target cells in the undiluted blood sample; contacting the binding agent-attached target cells in the undiluted blood sample with a plurality of buoyant reagents that include a second linker capable of binding to the first linker to generate an undiluted buoyant reagent-attached target cell mixture; diluting the undiluted buoyant reagent-attached target cell mixture by at least 20% to produce a diluted buoyant reagent-attached target cell mixture; applying a vectorial force, such as centrifugal force, to the diluted buoyant reagent-attached target cell mixture to generate a stratified diluted buoyant reagent-attached target cell mixture; removing the buoyant reagent-attached target cells from the stratified diluted buoyant reagent-attached target cell mixture; and isolating the target cells from the buoyant reagent-attached target cells.

Disclosed herein are methods for isolating target cells from blood, involving mixing in an open container an undiluted blood sample having a volume of 10 ml or less, and binding agents, wherein each binding agent comprises (A) a primary binding agent comprising an agent capable of binding to at least one cellular epitope on target cells in the undiluted blood sample, (B) a first linker bound to the primary binding agent, to generate binding agent-attached target cells in the undiluted blood sample; contacting the binding agent-attached target cells in the undiluted blood sample with a plurality of buoyant reagents that include a second linker capable of binding to the first linker to generate an undiluted buoyant reagent-attached target cell mixture; diluting the undiluted buoyant reagent-attached target cell mixture by at least 20% to produce a diluted buoyant reagent-attached target cell mixture; applying a vectorial force, such as centrifugal force, to the diluted buoyant reagent-attached target cell mixture to generate a stratified diluted buoyant reagent-attached target cell mixture; removing the buoyant reagent-attached target cells from the stratified diluted buoyant reagent-attached target cell mixture; and isolating the target cells from the buoyant reagent-attached target cells.

The present invention relates to apparatus for simultaneous grinding and froth flotation of at least one crude mineral and/or pigment, a process carried out in the apparatus for manufacturing at least one ground mineral and/or pigment, use of the ground mineral and/or pigment bearing phase obtainable by the process in paper applications as well as in paper, plastics, paints, coatings, adhesives, sealants, food, feed, pharma, concrete, cement, cosmetic, water treatment and/or agriculture applications, preferably in a wet end process of paper machine, in cigarette paper, board, and/or coating applications, or as support for rotogravure and/or offset and/or ink jet printing and/or continuous ink jet printing and/or flexography and/or electrophotography and/or decoration surfaces and the ground mineral and/or pigment bearing phase or ground mineral and/or pigment obtainable by the process.

The invention relates to a magneto-centrifugal flotation cell for ore concentration which reduces water consumption. A disadvantage of conventional flotation cells is the use of a large amount of water, some flotation cells requiring at least 60% water. The present invention uses ore pulp with increased density and viscosity, owing to the application of an axial magnetic field, wherein the Lorentz force, which is the force exerted by an electromagnetic field that receives a charged particle or an electrical current, can be used. The solution is a cell which, in addition to the forces that usually act on conventional flotation cells, uses external forces which, in principle, produce synergy in the separation of ore particles that have different gravitational and magnetic properties.

An apparatus for separating hydrocarbons from water, comprising a tank segment with a fluid inlet subsequent a housing with a liquid gas mixer. The fluid inlet through a cylindrical tank wall,and further through the wall of a conical wall segment, a pipe spread, combining radial and axial flow over ring mounted guide vanes at the upper part of the conical wall segment. A hydro carbon outlet is located at a top end and a water outlet at a bottom end of tank-segment. A pipe for scale and debris removal is connected to the lower part of the bowl. The inner conical wall segment extends to the top end of the tank segment and at this location comprises a ring of vertically mounted guide vanes.

Some implementations can include method and system to collect and remove micro plastics from a water environment or ecosystem.

A wide-size-fraction flotation system and process includes feeding coal slime to be floated into a stirrer, adding water to the floating coal slime in the stirrer, stirring, then feeding same into a grading cyclone through a first feeding pump for pre-grading; after grading of the coal slime in the grading cyclone, feeding overflow in the grading cyclone into a flotation column through a second feeding pump for flotation, discharging flotation tailings through an underflow port of the flotation column, collecting flotation concentrates through an overflow port of the flotation column and feeding same into a bubble generator through a fourth feeding pump, and the flotation concentrates passing through the bubble generator and being fed from the bottom of a hydraulic flotation machine; and feeding underflow in the grading cyclone into the hydraulic flotation machine through a third feeding pump, for flotation and recovery.

A dissolved air floatation apparatus including an ultrafine bubble-containing liquid production device and a dissolved air floatation tank. The ultrafine bubble-containing liquid production device includes a gas-liquid mixing unit and a bubble-containing liquid separation device. The bubble-containing liquid separation device swirls a liquid containing ultrafine bubbles and larger bubbles in a storage tank to concentrate the liquid that contains the ultrafine bubbles and the liquid that contains the larger bubbles to a central part of the swirling flow followed by discharge. A pressurized ultrafine bubble-containing liquid is mixed in a raw liquid containing a subject to be cleansed and is poured into the dissolved air floatation tank to cause a suspended substance and a dissolved component in the raw liquid to be adsorbed on an interface of fine bubbles and be floated in the dissolved air floatation tank to be extracted.

The present invention relates to apparatus for simultaneous grinding and froth flotation of at least one crude mineral and/or pigment, a process carried out in the apparatus for manufacturing at least one ground mineral and/or pigment, use of the ground mineral and/or pigment bearing phase obtainable by the process in paper applications as well as in paper, plastics, paints, coatings, adhesives, sealants, food, feed, pharma, concrete, cement, cosmetic, water treatment and/or agriculture applications, preferably in a wet end process of paper machine, in cigarette paper, board, and/or coating applications, or as support for rotogravure and/or offset and/or ink jet printing and/or continuous ink jet printing and/or flexography and/or electrophotography and/or decoration surfaces and the ground mineral and/or pigment bearing phase or ground mineral and/or pigment obtainable by the process.

The present invention discloses an oil-contained wastewater treatment apparatus applying the integrative physical methods. The wastewater treatment system of the invention may include a main tank, where the upper part is a rectangular body and the lower part is designed to a multi-bucket bottom structure. Two oil collection boxes are arranged to both outside ends of tank. A mud discharging outlet is attached to the bottom of the tank. Meanwhile, both of a water outlet and an electric polarizer are localized at the end face of the effluent on the tank. A power supply for the electro-adsorber is fixed to the inlet end on the top face of the tank. Divided by upper and lower deflectors, the inside of the tank is divided to three processing units, i.e., sludge-water separation unit, degradation-coalescence treatment unit, and sedimentation-electric polarization unit. Vortex centripetal gas flotation is applied to remove oil. Electro-adsorption induces the micelle clustering to achieve the decolorization. The electric polarization functions as anti-scaling, descaling, sterilization, and corrosion inhibition. Moreover, the referred physical treatment can be fulfilled in virtue of centrifugal force, buoyancy, gravity, adsorption force, coalescence force, inertia, shifting, and modification. Through the application of the system, the oil-contained wastewater can be treated environmentally friendly, safe and pollution-free. Besides the above advantages, high removal efficiency can make the apparatus and method a widely used approach on the oil-contained wastewater treatment.