A blood product (10), a method for preparing the blood product, a blood product obtainable by the method and a blood product preparing container means. The blood product comprises components from whole blood, especially fibrin, thrombocytes and leukocytes. The blood product (10) comprises a first layer (21), a second layer (22) and a third layer (23). The second layer (22) is adjacent to the first layer (21) and the third layer (23). The first layer (21) defines a first outer surface (24) of the blood product (10) and the third layer (23) defining a second outer surface (25) of the blood product (10). The first layer (21) comprises a majority of fibrin, the second layer (22) comprises a majority of thrombocytes and the third layer (23) comprises a majority of leukocytes.

A system for the processing and separation of biological fluids into components comprises an apparatus that cooperates with a disposable set, comprising a cabinet (100) for housing a hollow centrifugal processing chamber (20) of the disposable set. The cabinet comprises a plurality of side-by-side locations (110) for receiving a corresponding plurality of centrifugal processing chambers (20) in side-by-side spaced-apart relation. Each location comprises an individual drive means (52) for driving its centrifugal processing chamber. Remotely-actuable valves (124) associated with the disposable sets are located on the apparatus' cabinet in the proximity of said locations. Valve actuation provides a display of the state of actuation of the valves (124). Selection of this state of actuation is arranged to control connection of the centrifugal processing chamber (20) of each fitted disposable set with a flexible container (200) of the same disposable set or another container, and to control connection of the centrifugal processing chambers (20) with flexible containers of the same or other fitted disposable sets in different combinations, in particular with series and/or parallel connections.

A centrifugal separator includes disposable cartridge having a cylindrical bowl, a core tube assembly, and an annular piston disposed around the core tube assembly and inside the inner surface of the bowl. The disposable cartridge may be automatically loaded and withdrawn from the separator. Feed liquid is injected down the core tube assembly into the lower portion of the bowl, raising the annular piston, while the bowl rotates at high speed, separating solids from the feed liquid to accumulate along the inner surface of the bowl, while collecting clarified centrate as it exits the top of the bowl and through the core tube assembly. In a solids discharge mode, the annular piston is urged downward along a vertical axis to force accumulated solids from the bowl.

A process for the sequential processing of opaque and transparent biological fluids such as whole blood, apheresis blood, bone marrow blood, umbilical cord blood, buffy coat or cultured cells by processing steps in a hollow cylindrical centrifugal processing chamber (300) which is part of a disposable set. At least three different procedures selected from washing, incubation, transduction, separation, density gradient separation, dilution and volume adjustment are each carried out once or repeated a number of times according to a given processing profile in the processing chamber. Each procedure involves an input into the processing chamber, an operation in the processing chamber and an output from the processing chamber by displacement of a piston (310). The at least three different procedures are sequentially chained one after the other to constitute an overall sequential operation in the processing chamber and its disposable set. A first application is incubation for binding magnetic beads with human blood cells or stem cells. A second application is transduction by which foreign genetic material is inserted into human blood cells or stem cells by a virus. A third application is reconditioning biological fluids to achieve reproducible concentration and volumes of blood cells or stem cells.

Disclosed is a bioprocessing system comprising apparatus (200) including a centrifugal separation housing (210) having a temperature controllable compartment (215) for removably accepting a separation chamber (50), the apparatus further comprising at least one mixing station (250) for supporting one or more fluid storage vessels (10, 20, 30, 40), the station including a temperature controllable area (252) for increasing or decreasing the temperature of the contents of the or each supported vessel. The system further includes a disposable fluidic arrangement (100) including a centrifugal separation chamber (50) removably mountable within the compartment (215) and having one or more ports (52) allowing fluid ingress into, or egress out of the chamber, via the one or more ports in use, said ports being in fluid communication with one or more of said fluid storage vessels via fluid conduits (12, 22, 32, 42) and via one or more valve arrangements

A blood product (10), a method for preparing the blood product, a blood product obtainable by the method and a blood product preparing container means. The blood product comprises components from whole blood, especially fibrin, thrombocytes and leukocytes. The blood product (10) comprises a first layer (21), a second layer (22) and a third layer (23). The second layer (22) is adjacent to the first layer (21) and the third layer (23). The first layer (21) defines a first outer surface (24) of the blood product (10) and the third layer (23) defining a second outer surface (25) of the blood product (10). The first layer (21) comprises a majority of fibrin, the second layer (22) comprises a majority of thrombocytes and the third layer (23) comprises a majority of leukocytes.

A device for the processing and separation of biological fluids into components comprises a hollow centrifugal processing chamber (10) fitted with an inlet/outlet head (20) and preferably with an axially movable piston (18). The inlet/outlet head has two separate inlets/outlets, for instance an axial inlet (29) and a lateral outlet (40). The processing chamber (1) is fitted with an internal flow guide (30) enabling operation of the device in a continuous processing mode wherein biological fluid to be processed is continuously intaken by say the axial inlet (29) and at the same time processed components are continuously removed via say the lateral outlet (40). The continuous processing flow can be driven by an external peristaltic pump (59) and/or by axial displacement of a piston (18) in the chamber (10).

Provided is a centrifugation method for extracting a target component from an object including: providing a centrifugal container including a first space in an upper portion, a second space in a lower portion, a nozzle connecting the first space and the second space, a second plunger movably received in the second space, and a nozzle blocking member to penetrate the center of the second plunger in a longitudinal direction and to linearly move on the second plunger to selectively open/close a lower end of the nozzle; storing the object; interlayer-separating the object by primarily centrifuging the object; passing only the target component in the object; blocking the lower end of the nozzle by means of the nozzle blocking member; resuspending components remaining in the first space and the nozzle while the nozzle is blocked; and extracting a target component resuspended in the first space.

The present invention relates to a centrifugation device, a centrifugation method, and a separation container. More particularly, the present invention relates to a centrifugation device for separating substances from a body fluid and tissue using centrifugal force, the centrifugation device including: a separation container configured to receive, centrifuge, and clean a body fluid and tissue therein and rotate around a central rotation axis; a supply part connected to the separation container and configured to supply a cleaning solution to the separation container; a discharge part connected to the separation container and configured to receive substances separated by centrifugation; valves configured to control flows between the supply part, the discharge part, and the separation container; a driving device configured to rotate the separation container, the supply part, and the discharge part around the central rotation axis, the driving device being installed in a direction perpendicular to the separation container, the supply part, and the discharge part; and a controller configured to control an operation of the driving unit, wherein the separation container includes: a piston located outside in a radial direction based on the central rotation axis; and an elastic part placed on an outer side of the piston in the radial direction to push the piston in a direction opposite a centrifugal force direction.

The present invention relates to a system for separating biological fluids ink components that makes use of a hollow centrifugal processing chamber of variable volume. The system is a functionally closed centrifugation chamber that extracts sub-components of a biological fluid according to their density and size, such as platelets, plasma or red cells from whole blood.