A centrifugal field-flow fractionation device is provided with a controller for controlling introduction of a liquid sample by a sample introduction part and rotation of the rotor by a motor. The controller rotates the rotor at a first rotational speed when introducing the liquid sample into a flow path. The first rotational speed is faster than a second rotational speed. Therefore, when a liquid sample is introduced into the flow path, high centrifugal force can be applied to the liquid sample, which can suppress the variation of the particles in the liquid sample introduced to the flow path. The controller rotates the rotor at the second rotational speed slower than the first rotational speed with the liquid sample flow in the flow path stopped. Therefore, it is possible to prevent the constant application of high centrifugal force to the liquid sample, thereby suppressing the increase in the length of time required for analysis.

A fluid disposing system includes a centrifugal separator that centrifugally separates a liquid that is supplied, a reagent injecting apparatus coupled to the centrifugal separator and that injects a reagent into the centrifugal separator, a reagent supply module that supplies the reagent to the reagent injecting apparatus and a pipetting module provided on an upper side of the centrifugal separator and that feeds the fluid to the centrifugal separator.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

A rotor assembly (10, 150, 270, 310) and method of using the rotor assembly (10, 150, 270, 310). The rotor assembly (10, 150, 270, 310) includes a bio-process bag (48), a drum (46) that receives a lower portion of the bag (48), and a pressure ring (50). A holder (54, 182) couples an upper portion of the bag (48) to the pressure ring (50). The pressure ring (50) is coupled to the drum (46) to define an interior space that contains the bag (48). A liquid transport assembly (35, 178, 272) passes through an opening in the holder (54, 182) so that liquids can be added to, and removed from, the bag (48) without removing the rotor (16, 154) from the centrifuge. A bearing assembly (190) in the holder (54, 182) couples the liquid transport assembly (35, 178, 272) to the rotor (16, 154), and enables the liquid transport assembly (35, 178, 272) to remain stationary while the rotor (16, 154) rotates around it. One or more seal assemblies (276, 312) provide a fluid-tight seal against the outer portion of the liquid transport assembly (35, 178, 272), and prevent fluids from leaking from the bag (48) during centrifugation.

A cell washer is disclosed. The cell washer includes a vessel configured to hold cells. The vessel includes an elongated body including an opening, an inner surface, and a pocket defined by a first inner surface portion of the inner surface disposed between and radially outward relative to a second inner surface portion and a third inner surface portion of the inner surface, and a cavity. The vessel also includes an actuating device capable of causing the vessel to spin about an axis.

A fluid separation device includes a centrifugal separator configured to receive a centrifugal separation chamber of a disposable fluid flow circuit, a pump system configured to convey a fluid into the centrifugal separation chamber and to remove a separated fluid component from the centrifugal separation chamber via an outlet, a color-based interface monitoring system configured to determine an interface position between separated fluid components continuously flowing through the centrifugal separation chamber based on dominant wavelength measurements of layers of separated fluid components during a centrifugal separation procedure, and a controller configured to measure the dominant wavelengths of the layers, calculate a duration as a color time for each measured dominant wavelength, set target color times, calculate error signals and calculate control signals to adjust the pump system to control the flow rate and interface position.

Described are embodiments that include methods and devices for separating composite liquids into components. Embodiments involve the use of a flexible membrane for separating a composite liquid into components. The composite liquid may include, in embodiments, a cellular containing liquid, such as whole blood or components of whole blood. In one specific embodiment, the composite liquid is a buffy coat.

A processing device includes a pump system, a valve system, a centrifuge, and a controller. A fluid flow circuit is mounted to the device to execute a procedure in which whole blood is processed into a red blood cell product, a plasma product, and a platelet concentrate product. The blood is first separated into red blood cells, buffy coat, and plasma using the centrifuge, with the red blood cells and plasma being removed from the centrifuge, while the buffy coat remains in the centrifuge. The fluid remaining in the centrifuge is circulated through the centrifuge to form a homogenous mixture. Once the mixture is formed, it is separated in the centrifuge into platelet concentrate and red blood cells. A platelet product is then collected by using whole blood or previously collected red blood cells to push the platelet concentrate from the centrifuge to a collection container.

A centrifugal separation container which is revolved around a rotation axis, includes: a separation part which includes a distal region which is disposed on a distal side than a liquid-to-be-treated supply port and a proximal region which is disposed on a proximal side than the liquid-to-be-treated supply port, with respect to the rotation axis, and in which a liquid-to-be-treated discharge port is provided in the proximal region; and a recovery part which is disposed on a distal side than the distal region with respect to the rotation axis, communicates with a distal end portion of the distal region through a communication path, and is filled with a recovery liquid for dispersing dispersoids that are to be centrifuged in a liquid to be treated.

A blood separation system is provided that includes a blood separation device that includes a centrifugal separator and a spinning membrane separator drive unit incorporated into a common case and a fluid flow circuit having both a separation chamber configured to be mounted in the centrifugal separator of the blood separation device and a spinning membrane separator configured to be received in the spinning membrane separator drive unit. In an exemplary procedure, the system is used to collect concentrated platelets and/or concentrated platelets and plasma, and to further permit harvesting of the mononuclear cells from the centrifugal separator at the conclusion of platelet collection, and transfer of the mononuclear cells to the spinning membrane separator.