Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

Systems and methods for separating hydrogen sulfide from carbon dioxide in a high-pressure mixed stream are disclosed herein. The methods include receiving the high-pressure mixed stream in an oxidation reactor and at an inlet pressure of at least 0.3 megapascals. The high-pressure mixed stream includes 0.01 to 5 mole percent hydrogen sulfide and at least 90 mole percent carbon dioxide. The methods further include oxidizing the high-pressure mixed stream with an oxidant to generate a high-pressure oxidized stream, includes oxidized hydrogen sulfide and carbon dioxide, at an oxidation pressure of at least 0.3 megapascals. The methods also include separating the high-pressure oxidized stream into an oxidized hydrogen sulfide product and a carbon dioxide product and generating the carbon dioxide product at a pressure of at least 0.3 megapascals. The systems include the high-pressure mixed stream, an oxidation reactor, and a separation assembly.

Systems and methods for separating hydrogen sulfide from carbon dioxide in a high-pressure mixed stream are disclosed herein. The methods include receiving the high-pressure mixed stream in an oxidation reactor and at an inlet pressure of at least 0.3 megapascals. The high-pressure mixed stream includes 0.01 to 5 mole percent hydrogen sulfide and at least 90 mole percent carbon dioxide. The methods further include oxidizing the high-pressure mixed stream with an oxidant to generate a high-pressure oxidized stream, includes oxidized hydrogen sulfide and carbon dioxide, at an oxidation pressure of at least 0.3 megapascals. The methods also include separating the high-pressure oxidized stream into an oxidized hydrogen sulfide product and a carbon dioxide product and generating the carbon dioxide product at a pressure of at least 0.3 megapascals. The systems include the high-pressure mixed stream, an oxidation reactor, and a separation assembly.

A method of controlling aquatic pests: introducing sulfurous acid into water having the aquatic pests to treat the water until the aquatic pests are controlled, wherein the water is selected from open water, flowing water, or water open to the atmosphere. The aquatic pests are controlled within a control distance, which is within a distance of less than a mile from the site of introducing the sulfurous acid. The water can be open water or contained water, and the method comprises producing the sulfurous acid at a site of sulfurous acid introduction into the water. The aquatic pest is a zebra mussel, an Asiatic clam, a bryozoan or others. The method can include introducing the sulfurous acid so that the treated water has a pH of between about 6 and 6.5. The method can include introducing the sulfurous acid so that the treated water includes sulfites, bisulfites, and/or sulfur dioxide.

Disclosed are compositions that comprise an organic electrochemical mediator and a gasotransmitter salt which converts into a gasotransmitter via electron transfer. Also disclosed are bandages and wound dressings comprising the subject gasotransmitters, and methods of making a gasotransmitters, comprising exposing the compositions to a voltage sufficient to reduce the water soluble organic mediator. Further disclosed are methods of treating a variety of trauma and disease states by applying a therapeutically effective amount of at least one gasotransmitter thereto, and methods of generating an effective dose of the gasotransmitter generated from electrolytic reaction involving the gasotransmitter-generating compositions.

Disclosed are compositions that comprise an organic electrochemical mediator and a gasotransmitter salt which converts into a gasotransmitter via electron transfer. Also disclosed are bandages and wound dressings comprising the subject gasotransmitters, and methods of making a gasotransmitters, comprising exposing the compositions to a voltage sufficient to reduce the water soluble organic mediator. Further disclosed are methods of treating a variety of trauma and disease states by applying a therapeutically effective amount of at least one gasotransmitter thereto, and methods of generating an effective dose of the gasotransmitter generated from electrolytic reaction involving the gasotransmitter-generating compositions.

Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

Disclosed are compositions that comprise an organic electrochemical mediator and a gasotransmitter salt which converts into a gasotransmitter via electron transfer. Also disclosed are bandages and wound dressings comprising the subject gasotransmitters, and methods of making a gasotransmitters, comprising exposing the compositions to a voltage sufficient to reduce the water soluble organic mediator. Further disclosed are methods of treating a variety of trauma and disease states by applying a therapeutically effective amount of at least one gasotransmitter thereto, and methods of generating an effective dose of the gasotransmitter generated from electrolytic reaction involving the gasotransmitter-generating compositions.

Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.