The present invention relates to a microorganism mixture comprising one or more microorganisms selected from the Bacillus, Paenibacillus, lactobacillus, Pseudomonas, Trichoderma, aspergillus, and Saccharomyces genera, and which is useful in treating of solid organic waste. The present invention also relates to a composition comprising said microorganism mixture, a process for preparing said composition, a method for treating solid organic waste, and the use of the microorganism mixture or the composition of the invention in treating solid organic waste.

The invented bio-electrical system is a housing-electrode which allows insertion of another electrode for various electrochemical and bio-electrical applications. Together with other invented elements as well as standard components, the system is fully scalable, modular, and allows production and collection of gases under pressure. It can be built in many shapes, such as the embodied tubular shape. The design allows operation on unstable ground, for example on ships. Flow of electrolyte can be regulated and directed in cascaded reactions by opening and closing the compartments of the outer or the inner electrodes using the provided electrode holders. The redox conditions inside the system can be controlled using off-the-shelf power supplies which are controlled using the provided algorithm. Gas collection can be regulated based on the level of liquid inside the system using the provided float switches or conductivity probes even as the system is moving or operated under zero-gravity conditions.

A permeable reactive barrier having two or more layers of a geotextile fabric inoculated with a bioremediation microbe is provided. The permeable reactive barrier further includes two or more layers of coarse-grained geological material separating the two or more layers of geotextile fabric such that any pair of adjacent layers of geotextile fabric is separated by a layer of coarse-grained geological material. The permeable reactive barrier includes a perforated metal casing surrounding and containing the layers of coarse-grained geological materials and geotextile fabric.

A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or more Bacillus species, and heating to a preferred elevated temperature range of 41° C. to 44° C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.

A method for reducing toxic mercury in wastewater effluent comprises the steps of: identifying a system into which wastewater effluent is introduced, the wastewater effluent including organic compounds and organomercurial compounds; producing a treatment composition comprising a solution including a surfactant, digestive microbes suspended in the solution, and mercury-transformative microbes suspended in the solution; and providing the treatment composition into the system containing the wastewater effluent, such that the digestive microbes degrade the organic compounds in the wastewater effluent, and the mercury-transformative microbes reduce the organomercurial compounds in the wastewater effluent into nontoxic volatile elemental mercury. In certain systems for reducing toxic mercury in wastewater effluent, a biological capture medium is positioned within a vessel and configured to provide a capture point for microbes to adhere to and create biofilms. One such system is a dental evacuation system in which wastewater effluent is introduced into the system via an aspirator.

A method for improving the quality of pond water used in aquaculture applications by adding to the pond water active bacteria that are preferably germinated from spores on site using a nutrient-germinant composition and an incubation method for increased spore germination efficiency, in combination with a nitrification enhancement agent such as calcium carbonate or calcified seaweed, and an optional reaction surface area modifier such as calcified seaweed or plastic or metal particles or fragments. The nutrient-germinant composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores (preferably of one or more Bacillus species) or they may be separately combined for germination. The incubation method comprises heating a nutrient germinant composition and bacteria spores, to a temperature range of 35° C. to 60° C. for around 2 to 60 minutes to produce an incubated bacteria solution that is discharged to the aquaculture application.

A Septic Aid system includes at least tablet containing dissolvable septic treatment so constituted as to supply a septic system with replacement bacteria and/or enzymes. The at least one tablet is configured to slowly release the septic treatment into toilet water. The at least one tablet may also have a dissolvable color indicator configured to run out at approximately the same time as the dissolvable septic treatment is depleted. An attachment may be provided in the form of an adhesive disc, suction cup, or hangar. An applicator may be used to apply the at least one tablet to an interior surface of the toilet.

A spore germination composition and method to produce a bioaugmentation solution that is added to an anaerobic digester or partially aerobic digester to increase biogas production. A nutrient-germinant composition comprises L-amino acids a phosphate buffer, an industrial preservative, and an optional source of potassium. The composition and spores of one or more Bacillus species are heated to a preferred elevated temperature range of 35° C. to 60° C. for an incubation period of around 20 to 60 minutes to form a bioaugmentation solution that is dispensed to the digester, preferably to the hydrolysis stage of the digester. A dose of bioaugmentation solution is added to the digester around once per day in an amount to provide at least 1000 CFU per mL of the full volume capacity of the digester, which can increase methane production by around 5 to 10% over operation of the digester without the bioaugmentation solution.

The present invention relates to a process and plant for treating feed water containing nitrate. The process includes, sorbing nitrate from the feed water onto an ion exchange resin to form a loaded resin and produce a treated water stream depleted in nitrate, regenerating the loaded resin so that the resin can be reused and produce a brine stream high in nitrate; and converting nitrate in the brine stream into molecular nitrogen gas with the assistance of a bioactive agent.

The present Invention relates to a new and novel process for treatment of wastewater that combines treatment methods that use Ballast Material (BM), Hydrothermal Carbonization (HTC), Hydrodynamic Cavitation (HDC), Probiotics (PB), acid, and Bio-Adsorbents (BA) to replace biological treatment of wastewater, specifically Activated Sludge Technology (AST).