Provided are a bacterium for degrading sludge having a 16S rRNA gene comprising a nucleotide sequence having 97% or more identity to the nucleotide sequence represented by SEQ ID NO: 1, a bacterium having a 16S rRNA gene comprising the nucleotide sequence represented by SEQ ID NO: 1 with mutations of two bases or less, and having an ability to degrade a target microorganism, and a microbial preparation for degrading a target microorganism comprising a bacterium (a1) below. Bacterium (a1) is a bacterium having a 16S rRNA gene comprising a nucleotide sequence having 90% or more identity to the nucleotide sequence represented by SEQ ID NO: 1.

The present disclosure discloses a method for removing TBBPA in water, a microbial strain and a microbial agent, wherein the microbial strain is a domesticated Burkholderia cepacia strain, which is named Y17 with a conservation number GDMCC No. 62153. The microbial agent and the method for removing TBBPA in water with the microbial agent are that Y17 strains are colonized on the surface and pore channels of biochar, TBBPA in water is used as a carbon source, air and dissolved oxygen are used as oxygen sources, biochar provides the strains a growth microenvironment for degrading TBBPA in water, the strains are subjected to aerobic growth in water, and bio-enhanced degradation of TBBPA in water is performed by continuously degradation reaction. The removal method and the microbial strain as well as the microbial agent are high in degradation efficiency, environmental-friendly and low in cost, and can meet requirements on large-range promotion and application.

Provided are a biomineralogical method for removing cesium ions. The method for removing cesium ions, the method comprising: adding metal-reducing bacteria, an iron source, and a sulfur source into a solution containing the cesium ions to convert the cesium ions into a solid mineral incorporating cesium. The method for removing cesium ions according to the present invention has advantages in that the cesium ions may be removed with high efficiency and small volume even in the case in which competing ions are present at a high concentration like sea water.

Provided are a biomineralogical method for removing cesium ions. The method for removing cesium ions, the method comprising: adding metal-reducing bacteria, an iron source, and a sulfur source into a solution containing the cesium ions to convert the cesium ions into a solid mineral incorporating cesium. The method for removing cesium ions according to the present invention has advantages in that the cesium ions may be removed with high efficiency and small volume even in the case in which competing ions are present at a high concentration like sea water.

An anaerobic digestion system may include a material grinding/pulping portion, a hydrolysis portion arranged downstream of the grinding portion, a multiple chamber anaerobic reactor arranged downstream from the hydrolysis portion and including a gas collection and reintroduction system, a collection system for collecting digestate and gas from the anaerobic reactor.

In some embodiments, the present invention provides a recombinant protein comprising an affinity tag configured to attach the recombinant protein to a silicate surface, fused to a hydrogen sulfide scavenging enzyme.

The present disclosure relates to bioremediation systems and methods for wastewater treatment in heavy industry, including the mining industry. A benefit of the systems and methods disclosed herein can include the reduction of heavy metals in wastewater. Another benefit can be the treatment of acidic wastewater to achieve higher pH levels. An additional benefit can be the use of carbon dioxide to raise the pH level of acidic wastewater, or to produce feedstocks for the growth of anaerobic or aerobic microorganisms that are capable of reducing a concentration of heavy metals in wastewater. A benefit of the systems and methods herein can include the treatment of acid mining drainage wastewater, as well as heavy metal removal from other industrial wastewater. Another benefit of the methods and systems disclosed herein can include reduction of excess carbon dioxide from the environment.

A method for deep treatment of household waste leachate by a biochemical process is provided, including: arranging one anoxic tank and two aerobic tanks in series; introducing the household waste leachate into the primary anoxic reactor, and diluting the household waste leachate to an concentration acceptable to microorganisms; introducing the diluted household waste leachate into the primary aerobic reactor, and subjecting the diluted household waste leachate to an pre-nitrification reaction to obtain a reactant; introducing the reactant into the secondary aerobic reactor, and subjecting the reactant to a main nitrification reaction to convert ammonia nitrogen into nitrate nitrogen and nitrite nitrogen by nitrification of nitrobacteria; refluxing the nitrification liquid to the primary anoxic reactor, converting the nitrate nitrogen and nitrite nitrogen into nitrogen gas by denitrobacteria in the primary anoxic reactor, and discharging the nitrogen gas into atmosphere, thereby finishing an denitrification process.

Conventional methods for treating fat, oil, grease (FOG) and other build-up in wastewater systems (including grease traps) of restaurants and the like typically rely on chemical-based detergents, which may be damaging both to the environment and to the wastewater system itself. While some bio-friendly alternatives are known, a common problem with all these agents is that they are “flushed through” the system rapidly, and thus are relatively ineffective and inefficient. In providing an onsite system and method comprising cultivating micro-organisms and then using a carrier to deliver them to an affected environment, the present invention provides a solution that is more efficient in requiring less “starter” ingredients as well as more effective in ensuring the cultivated micro-organisms are delivered to, maintain sustained contact with, and have adequate time to treat, the undesirable substance(s).

The present invention refers to the heterologous production in microorganisms of modified versions of a predicted isoform of the surfactant protein Lv-ranaspumin-1 (Lv-Rsn-1), whose sequence was inferred from analyzes of the protein extract of the nest foam from the Northeastern Pepper Frog (Leptodactylus vastus). More specifically, it refers to two surfactant proteins that consist of modified versions of the predicted isoform of Lv-Rsn-1; to two synthetic genes each encoding one of these modified versions of the predicted isoform of Lv-Rsn-1; to two expression cassettes each containing one of the synthetic genes encoding one of the modified versions of the predicted isoform of Lv-Rsn-1; to two expression vectors each containing one of the synthetic genes encoding modified versions of the predicted isoform of Lv-Rsn-1; and to two transgenic microorganisms, a bacterium and a yeast, each transformed with one of these synthetic genes and heterologously producing one of the modified versions of the predicted isoform of Lv-Rsn-1. Lv-Rsn-1 has surfactancy, emulsification and dispersancy properties, among others, and its heterologous production allows it to be used in various applications and industrial products, without the need to extract it from the frog nest foam.