Provided are a method for producing a terminal conjugated dienal compound without an oxidation reaction and a terminal hydroxyacetal compound useful as an intermediate in the method. More specifically, provided are a method for producing an (E)-dienal compound comprising the steps of: a metalation reaction of an alkynal acetal compound (1) to obtain an organic metal compound (2), an addition reaction of (2) to ethylene oxide to obtain a hydroxyalkynal acetal compound (3), a reduction reaction of (3) to obtain an (E)-hydroxyalkenal acetal compound (4), a functional group conversion reaction of (4) to obtain an (E)-alkenal acetal compound (5) having a leaving group X, an elimination reaction of (5) to obtain an (E)-dienal acetal compound (6), and a hydrolysis reaction of (6) to obtain the (E)-dienal compound (7); and others.

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Provided are a method for producing a terminal conjugated dienal compound without an oxidation reaction and a terminal hydroxyacetal compound useful as an intermediate in the method. More specifically, provided are a method for producing an (E)-dienal compound comprising the steps of: a metalation reaction of an alkynal acetal compound (1) to obtain an organic metal compound (2), an addition reaction of (2) to ethylene oxide to obtain a hydroxyalkynal acetal compound (3), a reduction reaction of (3) to obtain an (E)-hydroxyalkenal acetal compound (4), a functional group conversion reaction of (4) to obtain an (E)-alkenal acetal compound (5) having a leaving group X, an elimination reaction of (5) to obtain an (E)-dienal acetal compound (6), and a hydrolysis reaction of (6) to obtain the (E)-dienal compound (7); and others.

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The invention relates to methods of separating or isolating a component from a plant using freeze separation. The invention includes products produced by said methods.

The invention relates to methods of separating or isolating a component from a plant using freeze separation. The invention includes products produced by said methods.

The invention relates to methods of separating or isolating a component from a plant using freeze separation. The invention includes products produced by said methods.

The invention relates to methods of separating or isolating a component from a plant using freeze separation. The invention includes products produced by said methods.

A method for preparing 2,2′,4,4′-Tetrahydroxy-3-(2″-hydroxy-3″-methylbutyl-3″-alkenyl)chalcone includes the following steps: subjecting a Morus alba leaf to extraction with an aqueous solution of methanol or ethanol having a volume fraction of 40%-100%, concentrating an extract to remove methanol or ethanol and dissolving in water, subjecting to extraction with petroleum ether and ethyl acetate successively, and concentrating an ethyl acetate extract to obtain a paste; chromatographing the paste over a silica gel column using chloroform-methanol, collecting an eluate where the volume ratio of chloroform-methanol is 95/5; chromatographing the eluate over a reversed-phase column using methanol-water, collecting an eluate where the volume ratio of methanol-water is 60/40, and thereby the compound is obtained.

A method for preparing 2,2′,4,4′-Tetrahydroxy-3-(2″-hydroxy-3″-methylbutyl-3″-alkenyl)chalcone includes the following steps: subjecting a Morus alba leaf to extraction with an aqueous solution of methanol or ethanol having a volume fraction of 40%-100%, concentrating an extract to remove methanol or ethanol and dissolving in water, subjecting to extraction with petroleum ether and ethyl acetate successively, and concentrating an ethyl acetate extract to obtain a paste; chromatographing the paste over a silica gel column using chloroform-methanol, collecting an eluate where the volume ratio of chloroform-methanol is 95/5; chromatographing the eluate over a reversed-phase column using methanol-water, collecting an eluate where the volume ratio of methanol-water is 60/40, and thereby the compound is obtained.

The disclosure relates to a device for fermentation integrated with separation and purification of acetone, butanol, and ethanol (ABE) or butanol alone, including a medium tank (1), used for supplying a medium into a bioreactor; a bioreactor (2), connected with the medium tank (1), used for fermentation; a gas distributor (9), used for supplying gas bubble to the fermentation broth; a membrane separation unit (4), with gas communication to the bioreactor (2), used for receiving a gas with ABE or butanol from the bioreactor and separating ABE or butanol; a condensation unit (5), used for recovering ABE or butanol; a vacuum manometer (6) and a vacuum pump (8), used for supplying a force for driving ABE or butanol in a vapor form; and product tank (7), used for receiving a product.

The disclosure relates to a device for fermentation integrated with separation and purification of acetone, butanol, and ethanol (ABE) or butanol alone, including a medium tank (1), used for supplying a medium into a bioreactor; a bioreactor (2), connected with the medium tank (1), used for fermentation; a gas distributor (9), used for supplying gas bubble to the fermentation broth; a membrane separation unit (4), with gas communication to the bioreactor (2), used for receiving a gas with ABE or butanol from the bioreactor and separating ABE or butanol; a condensation unit (5), used for recovering ABE or butanol; a vacuum manometer (6) and a vacuum pump (8), used for supplying a force for driving ABE or butanol in a vapor form; and product tank (7), used for receiving a product.