The invention provides a composition comprising a proteinase or an enzymatically active fragment thereof, said proteinase comprising the amino acid sequence of SEQ ID NO: 1 or comprising an amino acid sequence which is at least about 70% identical to SEQ ID NO: 1, wherein i) the concentration of free calcium in said composition is ≤about 80 μM; or ii) the concentration of monovalent salt in said composition is ≥about 20 mM. Under such conditions, the proteinases and enzymatically active fragments thereof are inducibly thermolabile. The invention further provides samples comprising one or more polypeptides and a proteinase or an enzymatically active fragment thereof, said proteinase comprising the amino acid sequence of SEQ ID NO: 1 or comprising an amino acid sequence which is at least about 70% identical to SEQ ID NO: 1, wherein i) the concentration of free calcium in said sample is ≤about 80 μM; or ii) the concentration of monovalent salt in said sample is ≥about 20 mM. The invention further provides methods comprising the inactivation of such proteinases or enzymatically active fragments thereof, wherein said method comprises the step of heating the sample to inactivate said proteinase or enzymatically active fragment, and wherein i) the concentration of free calcium in said sample is ≤about 80 μM; or ii) the concentration of monovalent salt in said sample is ≥about 20 mM.

The invention provides a composition comprising a proteinase or an enzymatically active fragment thereof, said proteinase comprising the amino acid sequence of SEQ ID NO: 1 or comprising an amino acid sequence which is at least about 70% identical to SEQ ID NO: 1, wherein i) the concentration of free calcium in said composition is ≤about 80 μM; or ii) the concentration of monovalent salt in said composition is ≥about 20 mM. Under such conditions, the proteinases and enzymatically active fragments thereof are inducibly thermolabile. The invention further provides samples comprising one or more polypeptides and a proteinase or an enzymatically active fragment thereof, said proteinase comprising the amino acid sequence of SEQ ID NO: 1 or comprising an amino acid sequence which is at least about 70% identical to SEQ ID NO: 1, wherein i) the concentration of free calcium in said sample is ≤about 80 μM; or ii) the concentration of monovalent salt in said sample is ≥about 20 mM. The invention further provides methods comprising the inactivation of such proteinases or enzymatically active fragments thereof, wherein said method comprises the step of heating the sample to inactivate said proteinase or enzymatically active fragment, and wherein i) the concentration of free calcium in said sample is ≤about 80 μM; or ii) the concentration of monovalent salt in said sample is ≥about 20 mM.

The invention relates to the use of adsorb/desorb chromatography to prepare enriched compositions comprising rebaudioside B and/or rebaudioside D. Compositions with enriched rebaudioside-B and/or rebaudioside-D components may be prepared from Stevia-derived glycoside compositions using an adsorb-desorb chromatography process where the stationary phase of the chromatography bed comprises a macroporous neutral adsorbent resin.

The invention relates to the use of adsorb/desorb chromatography to prepare enriched compositions comprising rebaudioside B and/or rebaudioside D. Compositions with enriched rebaudioside-B and/or rebaudioside-D components may be prepared from Stevia-derived glycoside compositions using an adsorb-desorb chromatography process where the stationary phase of the chromatography bed comprises a macroporous neutral adsorbent resin.

The present invention relates to the isolation and purification of sialylated oligosaccharides from an aqueous medium in which they are produced.

The present invention relates to the isolation and purification of sialylated oligosaccharides from an aqueous medium in which they are produced.

The present disclosure provides an ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root, comprising the following steps: S1, material treatment, S2, cold soaking, S3 enzymatic hydrolysis, S4 extract extraction, and S5 ultrasonic-microwave synergistic extraction. The extraction method of the present disclosure extracts relatively high content of total saponins, and has relatively high yield of saponins and low content of impurities, and each step acts synergistically to solve the problems of relatively low total saponin content, more impumayrities and bubbling in the extraction process.

The present disclosure provides an ultrasonic-microwave synergistic extraction method of total saponins in beautiful millettia root, comprising the following steps: S1, material treatment, S2, cold soaking, S3 enzymatic hydrolysis, S4 extract extraction, and S5 ultrasonic-microwave synergistic extraction. The extraction method of the present disclosure extracts relatively high content of total saponins, and has relatively high yield of saponins and low content of impurities, and each step acts synergistically to solve the problems of relatively low total saponin content, more impumayrities and bubbling in the extraction process.

The solution provides a method for separating a Chinese traditional medicine composition. To explain a pharmacological effect mechanism of a medicine made of two or more components and scientific content in rules of compatibility among components of a compound medicine, systematic researches on the material basis is very necessary. Accordingly, deep researches are done on chemical components of the pharmaceutical composition in the solution, and eight compounds are separated, which are 10-O-(p-hydroxycinnamoyl)-adoxosidic acid, aloe-emodin-8-O-β-D-glucopyranoside, quercitrin, matairesinol-4′-O-glucoside, liquiritin apioside, epi-vogeloside, vogeloside and ethyl caffeate, which provides a new quality control method for the composition in the solution.

There is demand for a novel method for manufacturing a RebD crystallized product. The present invention provides a method for manufacturing a RebD-containing crystallized product, including: a step for mixing a stevia plant-derived crude product having a total steviol glycoside content of 50-95 mass %, and containing at least RebA and RebD, into a solvent containing methanol or ethanol so that the supersaturation of RebD at 10° C. is at least 10 and the supersaturation of RebA at 10° C. does not exceed 18, and adjusting a crystallization solution; and a step for cooling the crystallization solution under stirring and causing RebD to precipitate.