The invention is directed to a conjugate having the general formula (I)

##STR00001## Wherein AR and MU are repeating units of a polymer MU is a polymer modifying unit or band gap modifying unit that is evenly or randomly distributed along the polymer main chain, G1 and G2 stand for hydrogen, halogen or an antigen recognizing moiety, with the provision that at least one of G1 or G2 is an antigen recognizing moiety, a is 10 to 100 mol %, b is 0 to 90 mol % c is 1 to 10 000; with the provisio that a+b=100 mol % characterized in that AR is connected in the polymer chain via the 2,2′ or 3,3′ or 5,5′ or 6,6′ or 7,7′ or 8,8′ positions according to general formula (II)

##STR00002## Wherein the remaining positions 2,2′; 3,3′; 4,4′; 5,5′; 6,6′; 7,7′ and 8,8′ are substituted with same or different residues selected from the group consisting of H, SO.sub.2CF.sub.3, SO.sub.2R.sub.a, CF.sub.3, CCl.sub.3, CN, SO.sub.3H, NO.sub.2, NR.sub.aR.sub.bR.sub.c.sup.+, CHO, CORa, CO.sub.2Ra, COCl, CONRaRb, F, Cl, Br, I, R.sub.a, OR.sub.a, SR.sub.a, OCOR.sub.a, NR.sub.aR.sub.b, NHCOR.sub.a, CCR.sub.a, aryl-, heteroaryl-, C.sub.6H.sub.4OR.sub.a or C.sub.6H.sub.4NRaRb, with Ra-c independently hydrogen, alkyl-, alkenyl-, alkinyl-, heteroalkyl-, aryl-, heteroaryl-, cycloalkyl-, alkylcycloalkyl-, heteroalkylcycloalkyl-, heterocycloalkyl-, aralkyl- or a heteroaralkyl residue or (CH.sub.2)x(OCH.sub.2CH.sub.2)yO(CH.sub.2)zCH.sub.3, wherein x is an integer from 0 to 20; y is an integer from 0 to 50 and z is an integer from 0 to 20

The invention is directed to a conjugate having the general formula (I)

##STR00001## Wherein AR, MU and MU* are repeating units of a polymer and MU and MU* are polymer modifying units or band gap modifying units which are evenly or randomly distributed along the polymer main chain, G1 and G2 stand for hydrogen, halogen or an antigen recognizing moiety, with the provision that at least one of G1 or G2 is an antigen recognizing moiety, a is 10 to 100 mol %, b is 0 to 90 mol % c is 0.1 to 90 mol % d is 1 to 10 000; with the provisio that a+b+c=100 mol % characterized in that AR is connected in the polymer chain via the 2,2′ or 3,3′ or 4,4′ or 5,5′ or 6,6′ or 7,7′ or 8,8′ positions according to general formula (II)

##STR00002## Wherein the remaining positions 2,2′; 3,3′; 4,4′; 5,5′; 6,6′; 7,7′ and 8,8′ are substituted with same or different residues selected from the group consisting of H, SO.sub.2CF.sub.3, SO.sub.2R.sub.a, CF.sub.3, CCl.sub.3, CN, SO.sub.3H, NO.sub.2, NR.sub.aR.sub.bR.sub.c.sup.+, CHO, CORa, CO.sub.2Ra, COCl, CONRaRb, F, Cl, Br, I, R.sub.a, OR.sub.a, SR.sub.a, OCOR.sub.a, NR.sub.aR.sub.b, NHCOR.sub.a, CCR.sub.a, aryl-, heteroaryl-, C.sub.6H.sub.4OR.sub.a or C.sub.6H.sub.4NRaRb, with Ra-c independently hydrogen, alkyl-, alkenyl-, alkinyl-, heteroalkyl-, aryl-, heteroaryl-, cycloalkyl-, alkylcycloalkyl-, heteroalkylcycloalkyl-, heterocycloalkyl-, aralkyl- or a heteroaralkyl residue or (CH.sub.2).sub.x(OCH.sub.2CH.sub.2).sub.yO(CH.sub.2).sub.zCH.sub.3, wherein x is an integer from 0 to 20; y is an integer from 0 to 50 and z is an integer from 0 to 20.

The invention is directed to a conjugate having the general formula (I) With AR, MU and L1 as repeating units of a polymer MU is a polymer modifying unit or band gap modifying unit that is evenly or randomly distributed along the polymer main chain, L1 is an aryl or a heteroaryl group evenly or randomly distributed along the polymer, L2 is an aryl or a heteroaryl group located on the ends of the polymer, FL is a fluorescent moiety, G1 and G2 stand for hydrogen, halogen or an antigen recognizing moiety, with the provision than at least one of G1 or G2 is an antigen recognizing moiety, characterized in that AR is connected in the polymer chain via the 2,2′ or 3,3′ or 5,5′ or 6,6′ or 7,7′ or 8,8′ position according to general formula (II)

##STR00001##

The present invention relates to a novel polyfluorene appended with protected glutamic acid of Formula (I) for heterogeneous enantioselective separation and sensing of amino acids, amino alcohol, hydroxyl acid, sugar, aromatic drug and ascorbic acid from racemic mixture in water and process for preparation thereof. The present invention further provides a process for separation of enantiomers and diastereomers into their individual isomers using a polyfluorene compounds of Formula (I). ##STR00001##

The present invention relates to a novel polyfluorene appended with protected glutamic acid of Formula (I) for heterogeneous enantioselective separation and sensing of amino acids, amino alcohol, hydroxyl acid, sugar, aromatic drug and ascorbic acid from racemic mixture in water and process for preparation thereof. The present invention further provides a process for separation of enantiomers and diastereomers into their individual isomers using a polyfluorene compounds of Formula (I).

##STR00001##